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Experimental Brain Injury Induces Regionally Distinct Apoptosis During The Acute And Delayed Post-Traumatic Period, Alana C. Conti, Ramesh Raghupathi, John Q. Trojanowski, Tracy K. Mcintosh
Experimental Brain Injury Induces Regionally Distinct Apoptosis During The Acute And Delayed Post-Traumatic Period, Alana C. Conti, Ramesh Raghupathi, John Q. Trojanowski, Tracy K. Mcintosh
Neurosurgery Faculty Publications
The temporal pattern of apoptosis in the adult rat brain after lateral fluid-percussion (FP) brain injury was characterized using terminal deoxynucleotidyl-transferase-mediated biotindUTP nick end labeling (TUNEL) histochemistry and agarose gel electrophoresis. Male Sprague Dawley rats were subjected to brain injury and killed for histological analysis at intervals from 12 hr to 2 months after injury (n = 3/time point). Sham (uninjured) controls were subjected to anesthesia with (n = 3) or without (n = 3) surgery. Apoptotic TUNEL-positive cells were defined using stringent morphological criteria including nuclear shrinkage and fragmentation and condensation of chromatin and cytoplasm. Double-labeled immunocytochemistry was performed …