Digital Commons Network^™

Non-Destructive, Rapid Differentiation Of Cell Types Relevant To Sexual Assault Investigations Utilizing Morphological And Autofluorescence Signatures, Taylor R. Moldenhauer

Theses and Dissertations

Current methods for confirming the presence of spermatozoa in sexual assault samples can be time-consuming and often lack sensitivity; however, this remains the most definitive test for the presence of semen. Additionally, male DNA can be deposited without the presence of intact sperm as may be the case with seminal fluid from vasectomized individuals or sexual activity where seminal fluid is not recovered (e.g., perpetrator wears a condom, penetration without ejaculation, etc.). The ability to detect bodily fluids, as well as quantify their presence in a sample, could aid in forensic DNA analysis by limiting the amount of time performing …

The Effect Of Time And Temperature On The Quality Of Latent Fingerprints On Incandescent Lightbulbs, Varying Donors Age And Sex, Kinaysha M. Collazo Maldonado

Theses and Dissertations

Fingerprints are used as a means of identification, but there are no established methodologies to determine time since deposition of latent fingerprints by visual means alone. This research considered the influence of age and sex on the quality of recovered latent prints from lit and unlit lightbulbs from 1 to 10 days, using accumulated degree hours (ADH) to account for both heat and time simultaneously. Two male and two female donors (one of each aged <40 and >40 years) were used. A thermal imaging camera was used to monitor the lightbulbs top and middle regions, which were significantly different (p≤0.05) for the …

Non-Invasive Retrieval Of Prefabricated Zirconia Crowns With Er,Cr:Ysgg Laser From Primary And Permanent Teeth, Connor W. Mccall Dds

Theses and Dissertations

Purpose: Compromised tooth structure on permanent and deciduous teeth in the pediatric population is increasingly being restored with tooth-colored prefabricated zirconia crowns. These restorations may need to be removed or replaced with permanent crowns. The purpose of this in vitro study was to explore the use of an Er,Cr:YSGG laser for removal of prefabricated zirconia crowns cemented with RMGI as a non-invasive alternative to rotary instruments.

Methods: Thirteen permanent and 12 primary molars were prepared to dentin and prefabricated zirconia crowns were passively fitted and cemented with two resin modified glass ionomer cements. The irradiation parameters for ErCR:YSGG were 4.5 …

Characterization Of The Dyrk1a Protein-Protein Interaction Network, Varsha Ananthapadmanabhan

Theses and Dissertations

Human Dual specificity tyrosine (Y)-Regulated Kinase 1A (DYRK1A) is a protein kinase encoded by a dosage-dependent gene. An extra copy of DYRK1A contributes to Down syndrome (DS) pathogenesis while loss of one allele causes severe mental retardation and autism. DYRK1A is involved in phosphorylation of several proteins that regulate cell cycle control and tumor suppression. However, the function and regulation of this kinase is not well understood and current knowledge does not fully explain dosage-dependent function of this important kinase. Our previous proteomic studies identified several novel DYRK1A interacting proteins including RNF169, FAM117B, TROAP, LZTS1, LZTS2 and DCAF7. In this …

Characterization Of The Tsc/Dyrk1a Interaction, Supriya Joshi

Theses and Dissertations

The Tuberous sclerosis complex (TSC) includes TSC1, TSC2 and the TBC1D7 subunits that together function as a principal inhibitor of the mTOR protein kinase complex 1 (mTORC1). mTORC1 is a master regulator of cell growth and proliferation that responds to signaling cues such as growth factors and nutrient availability. Proteomic studies in our lab revealed an interaction between the TSC subunits and DYRK1A, a ubiquitous protein kinase encoded by a gene located in the Down syndrome (DS) region on human chr21. In this study, we sought to validate the interaction of the TSC components with DYRK1A and to determine the …

Teachers' Perceptions Of The Broad Validity Of A High Stakes English Language Proficiency Test, Carolyn N. Waters

Theses and Dissertations

The No Child Left Behind Act of 2001 mandated that the English acquisition of all students identified as English learners be assessed annually using high-stakes standardized English language proficiency tests, and the Every Student Succeeds Act of 2015 continues this testing mandate. The WIDA ACCESS for ELLs® has been used for this and other purposes since 2005, and has been adopted by 40 State Education Agencies, including the Virginia Department of Education. However, despite the long-standing and widespread use of this assessment, no comprehensive independent evaluations of the test have been conducted. This mixed-methods study is a conceptual replication of …

Psychometric Developments Of The Ace-Iq: Understanding The Trauma History Of Latine Immigrants, Jorli K. Swingen Dr.

Theses and Dissertations

Adverse childhood experiences (ACEs) have a profound effect on an individual’s physical and mental health. The World Health Organization has recently updated the ACE questionnaire so it could be used with international populations. The Adverse Childhood Experiences-International Questionnaire (ACE-IQ), has not been translated to Spanish or used with Latine immigrants.

This study translated the ACE-IQ into Spanish, evaluated the psychometric properties of the questionnaire, and collected data on 184 four adult English Language Learners in Chesterfield County, VA.

There is evidence of internal consistency for the ACE-IQ as a whole (α = .908) and within subscales. Three factors were identified …

The Design Of A New Vaginal Applicator For Direction Modulated Brachytherapy (Dmbt) Using Geant4 Monte Carlo Simulation Code, Moeen Meftahi

Theses and Dissertations

Purpose: Anisotropy of the 192Ir source causes a lack of dose coverage at the apex in HDR vaginal cylinder brachytherapy (VC BT). In this study, we took advantage of the GEANT4 Monte Carlo (MC) simulation code to design a new DMBT vaginal applicator so that it maintains the dose coverage of the current vaginal cylinder at periphery everywhere while improving the apex coverage. In addition, since current treatment planning systems (TPS) considers the whole environment as water according to TG43 formalism, based on the capability of the MC in the calculation of dose in the material, the effect of inhomogeneity …

The Effect Of Print Angulation On The Accuracy And Precision Of 3d-Printed Orthodontic Retainers, Allison Williams

Theses and Dissertations

Purpose: The aims of this study were: 1) to compare the accuracy and precision of 3-

dimensional (3D) printed retainers at various angulations, 2) to evaluate the effect of angulation

on printing time and the amount of resin consumed.

Methods: Using a stereolithography (SLA) 3D printer, a total of 60 clear retainers were printed at five angulations (n=12, each): 15, 30, 45, 60, and 90 degrees. Six retainers were printed each cycle at a random order for all print angulations as print 1 and print 2. Digital images of the original and printed retainers were superimposed. Discrepancies on eight landmarks …

The Effects Of Autophagy And Senescence On Sensitivity To Cisplatin In Head And Neck Cancer, Zara H. Siddiqui

Theses and Dissertations

While current treatments in cancer, such as chemotherapy and radiation, can generally be effective in eliminating disease in patients, there also exists the possibility of recurrence of cancer cells over time. In patients diagnosed with locally advanced head and neck carcinoma, about 50-60% develop a loco-regional recurrence within two years, and 20-30% of patients develop metastatic disease at distant sites in the body [5]. On a cellular level, one mechanism for this survival may be that natural mechanisms such as autophagy and senescence play a role in allowing cells to survive after undergoing treatment. One standard of care chemotherapy for …

Extracellular Matrix Nanoparticles Effects On The Lung In Vivo, Brittaney E. Ritchie

Theses and Dissertations

Acute respiratory distress syndrome (ARDS) is a life-threatening condition that causes diffuse alveolar damage and a loss of the extracellular matrix (ECM). This leads to pulmonary edema and lung function deterioration. Our lab has created decellularized porcine lung, electrosprayed ECM nanoparticles that have been previously shown to have pro-regenerative capabilities in vitro.

In this study, the ECM nanoparticle effects on young murine lungs were tested in vivo. An ECM nanoparticle suspension, previously used for the in vitro studies, was aerosolized intratracheally into the lungs using a microsprayer. 24 hours later, the lung mechanics, bronchoalveolar lavage fluid, and histology …

Achieving Dentin Conservation Using Dynamic Navigation Technology For Locating Calcified Canals, Madison W. Saunders

Theses and Dissertations

Purpose: Pulp canal obliteration (PCO) is a common sequela of dental trauma, caries, restorations and vital pulp therapy procedures. Despite the application of high magnification and CBCT imaging, access cavity preparation for such cases is prone to procedural errors that may lead to substantial loss of dentin structure thereby reducing the long-term prognosis. This study aimed to achieve dentin conservation using a novel computer assisted dynamic navigation system (Navident) in comparison to freehand access preparation for locating calcified canals.

Methods: Forty maxillary and mandibular central incisors (tooth #9 and tooth #25) were 3-D printed to simulate PCO. They were randomly …

E-Liquid Exposure Causes Multiple Cardiovascular Defects In Xenopus Laevis Embryos, James E. Black Iii

Theses and Dissertations

Over the past several years vaping has become part of the cultural zeitgeist in the United States, with ECIG usage amongst those of reproductive age rising more and more. Due to a lack of regulation and research concerning their long term effects, public opinion trends toward the idea that they are somehow safer than their traditional counterparts. As cases of lung injury and ECIG related death are beginning to be reported more and more, it is clear that the misinformation about their safety is unfounded. Congenital heart defects (CHDs) are some of the most common birth defects currently in the …

The Correlation Between Maternal Postpartum Depression And Child Psychopathology, T'Keyah I. Vaughan

Theses and Dissertations

Background: Postpartum depression (PPD) is a phenomenon that affects nearly 10-15% of pregnancies in the US. It is characterized by depressed mood or anhedonia and lasting for more than 2 weeks. PPD changes how moms interact with family members and child-rearing behavior. Depression is a phenomenon that is also known to affect the psychopathology of children. However, the specifics of how postpartum depression impacts children remains controversial. Many studies do not control for major depressive disorder which makes it difficult to disentangle the impact depression has within the first year of life. Furthermore, other PPD risk factors may be confounding …

The Role Of Ca2+/Calmodulin Dependent Protein Kinase Ii, Camk-Ii, In Kidney Morphogenesis, Sarah R. Ingram

Theses and Dissertations

Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common heritable diseases in the world, characterized by the development of large fluid filled cyst. Treatments for this disease are limited due to a lack of understanding of disease pathogenesis. Ca²⁺/calmodulin dependent protein kinase II (CaMK-II) is necessary for kidney morphogenesis in zebrafish as well as being a downstream effector of pkd2, one of the genes most commonly mutated in ADPKD patients. The roles of CaMK-II during zebrafish kidney development include regulation of kidney cell migration as well as cloacal cilia stability. The influence of CaMK-II …

The Effect Of Virtual Reality Glasses On The Behavior Of Children With Autism Spectrum Disorder In The Dental Setting, Robert Lunka

Theses and Dissertations

Purpose: The study investigated if viewing a simulated dental exam on virtual reality glasses between two visits would desensitize participants with autism spectrum disorder to the dental environment and improve their anxiety and behavior.

Methods: This randomized prospective cohort study compared behavior between a Control Group and Experimental Group who wore the glasses between visits. Blinded raters watched video recordings of participants and assessed behavior using the Venham scale. Heart rate and post-visit questionnaires completed by guardians were also analyzed.

Results: Five subjects were enrolled (two in the Control and three in the Experimental groups). The median heart rate at …

Strategies And Devices For Improving Respiratory Drug Delivery To Infants And Children With Cystic Fibrosis, Karl Bass

Theses and Dissertations

Cystic Fibrosis (CF) is a degenerative disease, which causes thickening of the airway surface liquid and reduced mucociliary clearance, which provides an ideal habitat for bacterial infections. Early treatment of CF in children can prevent chronic infection, improve quality of life, and increase life expectancy. The most predominant bacteria found in CF-diseased lungs is Pseudomonas aeruginosa (Pa), which can be treated with inhaled tobramycin. Excipient enhanced growth (EEG) powder formulations are well suited for administering tobramycin to children, as the EEG approach provides minimal upper airway loss and targeted drug delivery. This method uses an initially small aerosol for high …

Synthesis Of Supported Metal Nanoparticles On High Surface Area Supports For Application In Energy Conversion And Heterogeneous Catalysis, Nazgol Norouzi

Theses and Dissertations

Precise control of metal nanoparticles’ size, composition, and dispersity over high surface area supports are highly desirable to address current challenges in energy storage and conversion as well as catalytic processes involving precious metals. Therefore, developing viable synthetic routes that enable new catalytic systems derived from inexpensive transition metals or limited use of precious metals is vital for clean energy applications such as fuel cells and rechargeable batteries or affordable drugs in the pharmaceuticals arena. In addition to metal components of heterogeneous catalysts, the catalyst support is an integral part of catalyst design as it can impart both physical stability …

Internalizing Data Collection: Personal Analytics As An Investigation Of The Self, Neal R. Swisher

Theses and Dissertations

Personal analytics, aka self-tracking, is the practice of using a digital device to track aspects of behavior, such as exercise habits, heart rate, sleep patterns, location, diet, and a host of other data points. This dissertation is an exploration of “self” in self-tracking, informed by theories of subjectivity, autonomy, power and knowledge. As a technological intervention, self-tracking devices change how we experience our own body and behavior. They also serve as methods to digitize human behavior. This data is combined with other data and processed using computational methods. Self-tracking devices are both personal and bureaucratic. They are devices used for …

The Moderating Role Of Developmental Assets On Relations Between Child Maltreatment, Trauma, And Dating Violence, Stephanie A. Hitti

Theses and Dissertations

Teenage dating violence (TDV) is prevalent and associated with an array of psychosocial and health problems. Thus, numerous studies have focused on the identification of risk and protective factors for TDV. Child maltreatment has been consistently identified as a risk factor for TDV victimization and perpetration. However, potential underlying mechanisms that may partially explain the relations between child maltreatment and TDV victimization and perpetration are not well understood. The present study explored indirect relations between child maltreatment and TDV victimization and perpetration via trauma symptoms, and examined whether internal and external developmental assets moderated relations between (a) child maltreatment and …

Examining Body Mass Index And Sepsis Mortality At One Year After Sepsis, Jamie D. Robinson

Theses and Dissertations

Introduction: Sepsis is a serious and life-threatening syndrome affecting 1.7 million Americans annually and resulting in approximately 270,000 deaths. An “obesity paradox” where obese individuals have lower sepsis mortality than their non-obese counterparts has been described. The problem is that the longevity of the effect is unknown, and few studies have examined the obesity paradox after 1-2 months post-diagnosis.

Methods: This retrospective cohort study examined clinical, demographic, and biomarker variables thought to affect sepsis mortality at three-time points: 30 days, 180 days, and at one year post-sepsis diagnosis in order to shed light on specific factors that might define …

Kavana: Photography, Jewish Storytelling, And Memory, Hannah Altman

Theses and Dissertations

Jewish thought suggests that the memory of an action is as primary as the action itself. This is to say that when my hand is wounded, I remember other hands. I trace ache back to other aches - when my mother grabbed my wrist pulling me across the intersection, when my great-grandmother’s fingers went numb on the ship headed towards Cuba fleeing the Nazis, when Miriam’s palms enduringly poured water for the Hebrews throughout their desert journey - this is how the Jew is able to fathom an ache. Because no physical space is a given for the Jewish diaspora, …

Transforming Trauma: Performing The Works Of William Shakespeare As Rehabilitation For Incarcerated Individuals, Elyssa Mersdorf

Theses and Dissertations

This paper is the summation of my research and exploration into the history, social ramifications, and individual psychological impact of incarceration and the use of theatre as a vehicle of rehabilitation. Throughout my studies, I encountered evidence in the forms of personal accounts from theatre practitioners, scholarly articles, inmate testimonials, and historical journals regarding the success of such carceral theatre programs in the reformation of the prisoners they serve. How have past prison procedures and strategies hindered or helped inmates in their preparation for their transition from life in a penitentiary to reintroduction into larger society? What are the financial …

...And Yet The Devil Exists, John Hee Taek Chae

Theses and Dissertations

...And Yet the Devil Exists is a project that explores the ways in which ideology determines reality. It is an installation that plots and connects the historical and personal narratives that have defined my sense of identity–narratives in which perceptions of reality shatter, mutate, or hybridize when confronted with power, opportunity, or coercion. The installation component of the project consists of three parts. The first is an infrastructure made of wooden beams upon which paintings and images are installed; I call this the lantern. In the center of this is a round table on top of which is a nonsensical …

Reaction Profiling Of Extracellular Protein Phosphorylation Through A Microfluidic Reactor Coupled With Raman Spectroscopy, Abigail Casey

Theses and Dissertations

Diseases and disorders in the human body are considered abnormalities of proper cellular function. Understanding the signal transduction mechanisms that cause these abnormalities is crucial to developing earlier detection methods, better treatment options and effective cures. While current diagnostic procedures are powerful tools in diagnosing diseases, they are ineffective in informing physicians on the real-time behavior of the signal transduction mechanisms associated with diseases and disorders. Currently, disease progression is monitored over time through routine patient visits and testing by one or more of the above techniques. By developing an approach that can monitor structural and conformational changes of proteins …

The Effects Of An Inflammasome Inhibitor Olt1177 On The Development Of Ischemic Heart Failure, Joseph S. Aliaga

Theses and Dissertations

Thesis Abstract

THE EFFECTS OF AN INFLAMMASOME INHIBITOR OLT1177 ON THE DEVELOPMENT OF ISCHEMIC HEART FAILURE

By Joseph S. Aliaga, BS

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at Virginia Commonwealth University.

Virginia Commonwealth University, 2020

Background: Evidence suggests that prolonged and enhanced pro-inflammatory signaling, modulated by the NLRP3 inflammasome, plays a crucial role in the pathophysiology of several different types of cardiovascular diseases such as acute myocardial infarction (AMI), adverse ventricular remodeling, and heart failure (HF). Consequently, we hypothesize that attenuating the enhanced inflammatory response using a pharmacological NLRP3 …

Food Park, Houria Boumzairig

Theses and Dissertations

If climate change and population growth progress at their current pace, in roughly 50 years farming as we know it will no longer exist (Despommier, 2009). This means that the majority of people could soon be without enough food or water. But there is a solution that is surprisingly within reach: Move most farming into cities, and grow crops in tall, specially constructed buildings. It’s called vertical farming. “There is a close relation between the beginning of agriculture and the birth of architecture. Our cities were shaped by food” (Precht, 2019). The agricultural revolution ended our presence as hunters and …

The Quantified Elf, Umico Niwa

Theses and Dissertations

The Quantified Elf is an ongoing collection of scientific, medical, archaeological articles and excerpts from my personal journal. It is a way by which I explore the various ways Western notions of personhood subsume human life into constructs of sexuality and gender, overlooking the various other modes of unbridled existence: plant, microbial. fungal, animal, celestial bodies. The speculative medical papers propose novel forms of body modification to combat gender dysphoria.

Spooky Stuff, Petra A. Szilagyi

Theses and Dissertations

A real imaginal exploration of the aesthetics of the supernatural.

The Program, Aaron Douglas Estrada

Theses and Dissertations

In my work, I explore the engineering of identity and socioeconomics that are programed into Black and Brown communities. I refer to this as “The Program”. The Program validates, breaks, codes, and critiques the system that divided us. The Program is identifying with the struggle of growing up in a system that is programmed to see you fail. So if you get caught up in that system it’s a natural death within the program. But you can break and make your own program. If you stick to the program.

The paper's use of language is coded. Text has been written …