Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Entire DC Network
Substrate Stiffness Controls Osteoblastic And Chondrocytic Differentiation Of Mesenchymal Stem Cells Without Exogenous Stimuli, Rene Olivares-Navarrete, Erin M. Lee, Kathryn Smith, Sharon L. Hyzy, Maryam Doroudi, Joseph K. Williams, Ken Gall, Barbara D. Boyan, Zvi Schwartz
Substrate Stiffness Controls Osteoblastic And Chondrocytic Differentiation Of Mesenchymal Stem Cells Without Exogenous Stimuli, Rene Olivares-Navarrete, Erin M. Lee, Kathryn Smith, Sharon L. Hyzy, Maryam Doroudi, Joseph K. Williams, Ken Gall, Barbara D. Boyan, Zvi Schwartz
Biomedical Engineering Publications
Stem cell fate has been linked to the mechanical properties of their underlying substrate, affecting mechanoreceptors and ultimately leading to downstream biological response. Studies have used polymers to mimic the stiffness of extracellular matrix as well as of individual tissues and shown mesenchymal stem cells (MSCs) could be directed along specific lineages. In this study, we examined the role of stiffness in MSC differentiation to two closely related cell phenotypes: osteoblast and chondrocyte. We prepared four methyl acrylate/methyl methacrylate (MA/MMA) polymer surfaces with elastic moduli ranging from 0.1 MPa to 310 MPa by altering monomer concentration. MSCs were cultured in β¦
Novel Osteogenic Ti-6al-4v Device For Restoration Of Dental Function In Patients With Large Bone Deficiencies: Design, Development And Implementation, David J. Cohen, A Cheng, A. Kahn, M. Aviram, Alexander J. Whitehead, Sharon Hyzy, Ryan M. Clohessy, Barbara D. Boyan, Zvi Schwartz
Novel Osteogenic Ti-6al-4v Device For Restoration Of Dental Function In Patients With Large Bone Deficiencies: Design, Development And Implementation, David J. Cohen, A Cheng, A. Kahn, M. Aviram, Alexander J. Whitehead, Sharon Hyzy, Ryan M. Clohessy, Barbara D. Boyan, Zvi Schwartz
Biomedical Engineering Publications
Custom devices supporting bone regeneration and implant placement are needed for edentulous patients with large mandibular deficiencies where endosteal implantation is not possible. We developed a novel subperiosteal titanium-aluminum-vanadium bone onlay device produced by additive manufacturing (AM) and post-fabrication osteogenic micro-/nano-scale surface texture modification. Human osteoblasts produced osteogenic and angiogenic factors when grown on laser-sintered nano-/micro-textured surfaces compared to smooth surfaces. Surface-processed constructs caused higher bone-to-implant contact, vertical bone growth into disk pores (microCT and histomorphometry), and mechanical pull-out force at 5 and 10βw on rat calvaria compared to non surface-modified constructs, even when pre-treating the bone to stimulate β¦
Regulation Of Osteoblast Differentiation By Acid-Etched And/Or Grit-Blasted Titanium Substrate Topography Is Enhanced By 1,25(Oh)2d3 In A Sex-Dependent Manner, Rene Olivares-Navarrete, Sharon L. Hyzy, Barbara D. Boyan, Zvi Schwartz
Regulation Of Osteoblast Differentiation By Acid-Etched And/Or Grit-Blasted Titanium Substrate Topography Is Enhanced By 1,25(Oh)2d3 In A Sex-Dependent Manner, Rene Olivares-Navarrete, Sharon L. Hyzy, Barbara D. Boyan, Zvi Schwartz
Biomedical Engineering Publications
This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1πΌ,25-dihydroxyvitamin D3 (1πΌ,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, π π = 0.87 πm), grit-blasted (GB, π π = 3.90 πm), or grit-blasted/acid-etched (SLA, π π = 3.22 πm) Ti. BMCs were sensitive to surface topography and underwent osteoblast differentiation. This was greatest on SLA; acid etching and grit blasting contributed additively. Primary osteoblasts were also sensitive β¦
Amelogenin Peptide Extract Increases Differentiation And Angiogenic And Local Factor Production And Inhibits Apoptosis In Human Osteoblasts, Rene Olivares-Navarrete, Sharon L. Hyzy, Argelia Almaguer-Flores, Corinna Mauth, Anja C. Gemperli, Barbara D. Boyan, Zvi Schwartz
Amelogenin Peptide Extract Increases Differentiation And Angiogenic And Local Factor Production And Inhibits Apoptosis In Human Osteoblasts, Rene Olivares-Navarrete, Sharon L. Hyzy, Argelia Almaguer-Flores, Corinna Mauth, Anja C. Gemperli, Barbara D. Boyan, Zvi Schwartz
Biomedical Engineering Publications
Enamel matrix derivative (EMD), a decellularized porcine extracellular matrix (ECM), is used clinically in periodontal tissue regeneration. Amelogenin, EMDβs principal component, spontaneously assembles into nanospheres in vivo, forming an ECM complex that releases proteolytically cleaved peptides. However, the role of amelogenin or amelogenin peptides in mediating osteoblast response to EMD is not clear. Human MG63 osteoblast-like cells or normal human osteoblasts were treated with recombinant human amelogenin or a 5βkDa tyrosine-rich amelogenin peptide (TRAP) isolated from EMD and the effect on osteogenesis, local factor production, and apoptosis assessed. Treated MG63 cells increased alkaline phosphatase specific activity and levels of β¦
Mineralization Potential Of Electrospun Pdo-Hydroxyapatite-Fibrinogen Blended Scaffolds, Isaac Rodriguez, Parthasarathy A. Madurantakam, Jennifer M. Mccool, Scott A. Sell, Hu Yang, Peter C. Moon, Gary L. Bowlin
Mineralization Potential Of Electrospun Pdo-Hydroxyapatite-Fibrinogen Blended Scaffolds, Isaac Rodriguez, Parthasarathy A. Madurantakam, Jennifer M. Mccool, Scott A. Sell, Hu Yang, Peter C. Moon, Gary L. Bowlin
Biomedical Engineering Publications
The current bone autograft procedure for cleft palate repair presents several disadvantages such as limited availability, additional invasive surgery, and donor site morbidity. The present preliminary study evaluates the mineralization potential of electrospun polydioxanone:nano-hydroxyapatiteβ:βfibrinogen (PDOβ:βnHAβ:βFg) blended scaffolds in different simulated body fluids (SBF). Scaffolds were fabricated by blending PDOβ:βnHAβ:βFg in the following percent by weight ratios: 100β:β0β:β0, 50β:β25β:β25, 50β:β50β:β0, 50β:β0β:β50, 0β:β0β:β100, and 0β:β50β:β50. Samples were immersed β¦