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A Study Of Nonenzymatic-Glycosylated-Low-Density-Lipoprotein Binding Using The Calf Adrenocortical Low-Density Lipoprotein Receptor., Peter. Catomeris
A Study Of Nonenzymatic-Glycosylated-Low-Density-Lipoprotein Binding Using The Calf Adrenocortical Low-Density Lipoprotein Receptor., Peter. Catomeris
Electronic Theses and Dissertations
In vitro studies have shown that nonenzymatic glycosylation of low-density lipoprotein (LDL) reduces its ability to bind the LDL receptor. It has been suggested that this may contribute to the increased risk of atherosclerosis associated with diabetes, since modified LDL is cleared from plasma by atherogenic pathways. In order to test the ability of LDL from diabetics to bind to the LDL receptor, a competitive binding assay was developed in which the calf adrenocortical LDL receptor was used as the binder. The ability of LDL to bind to the receptor was determined indirectly by its ability to displace control $\sp{125}$I-LDL …
Chapter~1. Alkylation Chemistry Of The Camphor Imine Of Tert-Butyl Glycinate And Its Derivatives. Chapter~2. Synthesis And Reactions Of A New Carbon(2) Symmetric Ketone., Kenneth C. Cassidy
Chapter~1. Alkylation Chemistry Of The Camphor Imine Of Tert-Butyl Glycinate And Its Derivatives. Chapter~2. Synthesis And Reactions Of A New Carbon(2) Symmetric Ketone., Kenneth C. Cassidy
Electronic Theses and Dissertations
Factors which influence the diastereoselectivity in the alkylation of the (R)-camphor imine of tert-butyl glycinate (24) were investigated in order to further refine the alkylation model. It was demonstrated that when alkylating 24 with various benzyl bromides or allyl bromide that the diastereoselectivity was dependent on the $\pi$-electron density of the alkylating agent, the metal cation of the enolate and the cosolvent used. Inferior selectivities (de's $$ 95%) were obtained when using HMPA as the cosolvent, Li or Na as the metal cation and alkylating agents with electron rich $\pi$-systems. Further study included the preparation of several camphor derivatives (ketopinic …
Investigation Of The Pathways Leading To Reversible And Irreversible Inactivation Of Horseradish Peroxidase., Kathy J. Baynton
Investigation Of The Pathways Leading To Reversible And Irreversible Inactivation Of Horseradish Peroxidase., Kathy J. Baynton
Electronic Theses and Dissertations
Verification of the purity of a commercial Horseradish peroxidase (HRP) preparation, kinetic analyses of two chromogenic assays and an investigation of the mechanisms of enzyme inactivation by two of its substrates, hydrogen peroxide ($\rm{H\sp2 O\sb2}$) and phenol, are described. Isoelectricfocusing of Boehringer Mannheim Grad II HRP preparation revealed that it is composed of neutral isozymes B and C, as reported by the Manufacturer. No other contaminating isoenzymes were detected. Kinetic analysis (T = 25$\sp\circ$C, pH 7.4) of the 4-amino-antipyrine (AAP)/3,5-dichloro-2-hydroxybenzenesulfonic acid (HDCBS) chromogen system yielded K$\sb{\rm mapp}$ values for $\rm{H\sb2 O\sb2}$, AAP and HDCBS of 41.0$\mu$M, 3.94mM and 1.4mM, respectively. …
Defluorination Of 4-Deoxy-4-Fluoro-D-Glucose In Pseudomonas Putida., Max Luis. Tejada
Defluorination Of 4-Deoxy-4-Fluoro-D-Glucose In Pseudomonas Putida., Max Luis. Tejada
Electronic Theses and Dissertations
Growth of P. putida using gluconate as a carbon source effects a considerable decrease in the rate of 4-deoxy-4-fluoro-D-glucose (4FG) metabolism to 2,3-dideoxy-D-glycero-pentonic acid (2,3-DDRA). In vivo $\sp{19}$F-NMR spectroscopic analyses of 4FG metabolism indicate that fluoride release is the initial step preceeding 4FG metabolism to 2,3-DDRA. Crude periplasmic extracts produced by osmotic shock lack fluoride release activity. This suggests that defluorination is not dependent upon the initial binding of a periplasmic protein to 4FG. The binding protein of P. putida lacks a binding affinity for 4FG as indicated by the absence of significant chemical shifting observed by $\sp{19}$F-NMR in incubations …