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Part I: Aldol Reactions Of 1,3-Oxathiolan-5-Ones. Part Ii: Camphor As A Chiral Directing Agent In Alkylations Of Glycinates., Pratibha. Mishra
Part I: Aldol Reactions Of 1,3-Oxathiolan-5-Ones. Part Ii: Camphor As A Chiral Directing Agent In Alkylations Of Glycinates., Pratibha. Mishra
Electronic Theses and Dissertations
Part I. 1,3-Oxathiolan-5-ones can be reduced to 2-mercaptoaldehydes which are synthetically important in the preparation of 2,5-dihydro-thiophenes and dienes.('1,2) Alkylation of 1,3-oxathiolan-5-ones was examined as a route to structurally more complex 2-mercaptoaldehydes. Alkylations were successful only with very reactive alkylating agents.('2,3) Aldol reactions were more successful. 1,3-Oxathiolan-5-ones were reduced with lithium aluminum hydride to 2-mercaptoalcohols, which could be transformed into the 2-(methylthio)alcohols. Part II. Camphor imines of glycinates were prepared from camphorthione and alkylated with various alkylating agents differing in steric requirements and reactivity. Diastereomeric excesses for alkylation with simple alkyl halides ranged from zero percent for methyl iodide to …
Transport Of 2-Keto-D-Gluconate And L-Malate In Pseudomonas Putida., Francisca Roseline Akuvi. Agbanyo
Transport Of 2-Keto-D-Gluconate And L-Malate In Pseudomonas Putida., Francisca Roseline Akuvi. Agbanyo
Electronic Theses and Dissertations
Both glucose- and succinate-grown whole cells of P. putida transport 2-keto-D-gluconate (2KGA) by a saturable process. The transport of 2KGA by membrane vesicles prepared from glucose-grown P. putida occurs by a facilitated diffusion process, with an apparent Km of 111.0 (+OR-) 2.9 (mu)M and a Vmax of 0.55 (+OR-) 0.04 nmol.mg protein.('-1)min.('-1). The provision of artificial and natural electron donors results in a significant decrease in Km value, while the Vmax remains virtually unchanged. 2KGA transport is inhibited by antimycin A, rotenone and DNP in the presence of L-malate/FAD. 4F2KGA and 3F2KGA also inhibit 2KGA transport competitively. With vesicles prepared …
I. A Fluorometric Coupled Enzymatic Method For The Determination Of Adenosine Triphosphate In Platelets. Ii. Colorimetric Determination Of Non-Enzymatically Glycated Albumin., Patrick Stephen Michael. Caines
I. A Fluorometric Coupled Enzymatic Method For The Determination Of Adenosine Triphosphate In Platelets. Ii. Colorimetric Determination Of Non-Enzymatically Glycated Albumin., Patrick Stephen Michael. Caines
Electronic Theses and Dissertations
Part I. A new fluorometric, coupled, enzymatic method for the determination of adenosine triphosphate is described. The procedure uses the hexokinase reaction coupled with three other enzymes in a system of phosphorylation and double reduction to form NADPH. The NADPH is measured in a reaction catalyzed by diaphorase in which the nonfluorescent material, resazurin, is converted to the highly fluorescent compound, resorufin. This kinetic method can determine adenosine triphosphate at the picomole level. Intra- and inter-assay CV's were both less than 3% and recoveries were quantitative. No significant interference was observed from EDTA or heparin. Correlation with an established method …