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A Robust Agroinfiltration Method, Bryce N. Trull
A Robust Agroinfiltration Method, Bryce N. Trull
Masters Theses
Stable transformation of soybean (Glycine max) is a markedly slow and laborious process. Thus, a tool that enables rapid evaluation of genetic elements in planta is critical to advance complex research and genetic engineering in soybean. To that end, a substantially robust agroinfiltration method was innovated in this work. Agroinfiltration is a technique that leverages Agrobacterium-mediated transient transformation to deliver genetic elements to the cells of whole plant tissues, usually leaves. Several factors were found to be relevant to successful soybean leaf agroinfiltration, including genotype, surfactant, developmental stage, and Agrobacterium culture medium. The research represents not only …
Role Of Kinases And An Epigenetically Regulated Gene In Soybean Cyst Nematode Resistance, Tracy Elizabeth Hawk
Role Of Kinases And An Epigenetically Regulated Gene In Soybean Cyst Nematode Resistance, Tracy Elizabeth Hawk
Doctoral Dissertations
Soybean cyst nematode (SCN) is the most devastating and economically important pathogen of soybean across the United States and globally. The current primary control for SCN is with the use of resistant cultivars, however, SCN populations are shifting to overcome most common sources of soybean resistance. This research was conducted to identify novel sources of genetic resistance to SCN. First, I functionally characterized two protein kinase hub genes whose function are predicted to be essential for mediating soybean susceptibility to SCN. I generated kinase-dead variants by mutating two amino acids required for the kinase enzymatic activity. Overexpression of the kinase-dead …