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Field-Grown Mir156 Transgenic Switchgrass Reproduction, Yield, Global Gene Expression Analysis, And Bioconfinement, Chelsea R. Johnson, Reginald J. Millwood, Yuhong Tang, Jiqing Gou, Robert W. Sykes, Geoffrey B. Turner, Mark F. Davis, Yi Sang, Zeng-Yu Wang, C. Neal Stewart Jr
Field-Grown Mir156 Transgenic Switchgrass Reproduction, Yield, Global Gene Expression Analysis, And Bioconfinement, Chelsea R. Johnson, Reginald J. Millwood, Yuhong Tang, Jiqing Gou, Robert W. Sykes, Geoffrey B. Turner, Mark F. Davis, Yi Sang, Zeng-Yu Wang, C. Neal Stewart Jr
Plant Sciences Publications and Other Works
Background
Genetic engineering has been effective in altering cell walls for biofuel production in the bioenergy crop, switchgrass (Panicum virgatum). However, regulatory issues arising from gene flow may prevent commercialization of engineered switchgrass in the eastern United States where the species is native. Depending on its expression level, microRNA156 (miR156) can reduce, delay, or eliminate flowering, which may serve to decrease transgene flow. In this unique field study of transgenic switchgrass that was permitted to flower, two low (T14 and T35) and two medium (T27 and T37) miR156-overexpressing ‘Alamo’ lines with the transgene under the control of the …
Transgenic Mir156 Switchgrass In The Field: Growth, Recalcitrance And Rust Susceptibility, Holly L. Baxter, Mitra Mazarei, Alexandru Dumitrache, Jace M. Natzke, Miguel Rodriguez Jr, Jiqing Gou, Chunxiang Fu, Robert W. Sykes, Geoffrey B. Turner, Mark F. Davis, Steven D. Brown, Brian H. Davison, Zen-Yu Wang, C. Neal Stewart Jr
Transgenic Mir156 Switchgrass In The Field: Growth, Recalcitrance And Rust Susceptibility, Holly L. Baxter, Mitra Mazarei, Alexandru Dumitrache, Jace M. Natzke, Miguel Rodriguez Jr, Jiqing Gou, Chunxiang Fu, Robert W. Sykes, Geoffrey B. Turner, Mark F. Davis, Steven D. Brown, Brian H. Davison, Zen-Yu Wang, C. Neal Stewart Jr
Plant Sciences Publications and Other Works
Sustainable utilization of lignocellulosic perennial grass feedstocks will be enabled by high biomass production and optimized cell wall chemistry for efficient conversion into biofuels. MicroRNAs are regulatory elements that modulate the expression of genes involved in various biological functions in plants, including growth and development. In greenhouse studies, overexpressing a microRNA (miR156) gene in switchgrass had dramatic effects on plant architecture and flowering, which appeared to be driven by transgene expression levels. Highexpressing lines were extremely dwarfed, whereas low and moderate-expressing lines had higher biomass yields, improved sugar release and delayed flowering. Four lines with moderate or low miR156 overexpression …