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Efficient Gene-Driven Germ-Line Point Mutagenesis Of C57bl/6j Mice, Edward J. Michaud, Cymbeline T. Culiat, Mitchell L. Klebig, Paul E. Barker, Kt Cain, Debra J. Carpenter, Lori L. Easter, Carmen M. Foster, Alysyn W. Gardner, Zy Guo, Kay J. Houser, Lori A. Hughes, Marilyn K. Kerley, Zhaowei Liu, Robert E. Olszewski, Irina Pinn, Ginger D. Shaw, Sarah G. Shinpock, Ann M. Wymore, Eugene M. Rinchik, Dabney K. Johnson
Efficient Gene-Driven Germ-Line Point Mutagenesis Of C57bl/6j Mice, Edward J. Michaud, Cymbeline T. Culiat, Mitchell L. Klebig, Paul E. Barker, Kt Cain, Debra J. Carpenter, Lori L. Easter, Carmen M. Foster, Alysyn W. Gardner, Zy Guo, Kay J. Houser, Lori A. Hughes, Marilyn K. Kerley, Zhaowei Liu, Robert E. Olszewski, Irina Pinn, Ginger D. Shaw, Sarah G. Shinpock, Ann M. Wymore, Eugene M. Rinchik, Dabney K. Johnson
Faculty Publications and Other Works -- General Biology
Background
Analysis of an allelic series of point mutations in a gene, generated by N-ethyl-N-nitrosourea (ENU) mutagenesis, is a valuable method for discovering the full scope of its biological function. Here we present an efficient gene-driven approach for identifying ENU-induced point mutations in any gene in C57BL/6J mice. The advantage of such an approach is that it allows one to select any gene of interest in the mouse genome and to go directly from DNA sequence to mutant mice.
Results
We produced the Cryopreserved Mutant Mouse Bank (CMMB), which is an archive of DNA, cDNA, tissues, and …
Oogenesis In Cultures Derived From Adult Human Ovaries, Antonin Bukovsky, Marta Svetlikova, Michael R. Caudle
Oogenesis In Cultures Derived From Adult Human Ovaries, Antonin Bukovsky, Marta Svetlikova, Michael R. Caudle
Faculty Publications and Other Works -- General Biology
Ten years ago, we reported that in adult human females the ovarian surface epithelium (OSE) is a source of germ cells. Recently, we also demonstrated that new primary follicles are formed by assembly of oocytes with nests of primitive granulosa cells in the ovarian cortex. The components of the new primary follicles, primitive granulosa and germ cells, differentiated sequentially from the OSE, which arises from cytokeratin positive mesenchymal progenitor cells residing in the ovarian tunica albuginea. In the present study, we investigated the possibility that the oocytes and granulosa cells may differentiate in cultures derived from adult human ovaries. Cells …
Expression Of The Α7 Nicotinic Acetylcholine Receptor In Human Lung Cells, Howard K. Plummer, Madhu Dhar, Hildegard M. Schuller
Expression Of The Α7 Nicotinic Acetylcholine Receptor In Human Lung Cells, Howard K. Plummer, Madhu Dhar, Hildegard M. Schuller
Faculty Publications and Other Works -- General Biology
Background
We and others have shown that one of the mechanisms of growth regulation of small cell lung cancer cell lines and cultured pulmonary neuroendocrine cells is by the binding of agonists to the α7 neuronal nicotinic acetylcholine receptor. In addition, we have shown that the nicotine-derived carcinogenic nitrosamine, 4(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), is a high affinity agonist for the α7 nicotinic acetylcholine receptor. In the present study, our goal was to determine the extent of α7 mRNA and protein expression in the human lung.
Methods
Experiments were done using reverse transcription polymerase chain reaction (RT-PCR), a nuclease protection assay and western …