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Carcinogenicity Of Hexavalent Chromium, Amie L. Holmes, S. S. Wise, John P. Wise Sr.
Carcinogenicity Of Hexavalent Chromium, Amie L. Holmes, S. S. Wise, John P. Wise Sr.
Applied Medical Sciences [Discontinued]
Hexavalent chromium (Cr(VI)), a commonly used industrial metal, is a well known human lung carcinogen. Epidemiology and animal studies suggest that the particulate Cr(VI) compounds, specifically the water insoluble compounds, are the more potent carcinogens, however, the carcinogenic mechanism remains unknown. Here we summarize recent Cr(VI)-induced human tumour, in vivo, cell culture and in vitro studies and put the data into context with three major paradigms of carcinogenesis: multistage carcinogenesis, genomic instability, and epigenetic modifications. Based on these studies, we propose a mechanism for chromate carcinogenesis that is primarily driven by the genomic instability paradigm.
Development Of Fragment-Specific Osteopontin Antibodies And Elisa For Quantification In Human Metastatic Breast Cancer, Alicia Plumer, Hongyi Duan, Sripriya Subramaniam, F. Lee Lucas, Susan Miesfeldt, Ah-Kau Ng, Lucy Liaw
Development Of Fragment-Specific Osteopontin Antibodies And Elisa For Quantification In Human Metastatic Breast Cancer, Alicia Plumer, Hongyi Duan, Sripriya Subramaniam, F. Lee Lucas, Susan Miesfeldt, Ah-Kau Ng, Lucy Liaw
Applied Medical Sciences [Discontinued]
Background: Osteopontin (OPN) is associated with human cancers, and circulating blood OPN may have diagnostic or prognostic value in clinical oncology. Methods: To evaluate OPN as a cancer biomarker, we generated and characterized five novel mouse monoclonal antibodies against the human full-length OPN (fl-OPN). Epitopes recognized by four antibodies (2C5, 2F10, 2H9, and 2E11) map to N-terminal OPN (aa1-166); one (1F11) maps to C-terminal OPN (aa167-314). These antibodies recognize recombinant and native OPN by ELISA and immunoblot, cross reacting with human and mouse OPN. Two of these novel antibodies ( 2F10 and 1F11) were used to develop a quantitative enzyme …