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Development Of A Ddpcr Multiplex To Measure The Immune Response To Borrelia Burgdorferi., Kailey Marie Mccain
Development Of A Ddpcr Multiplex To Measure The Immune Response To Borrelia Burgdorferi., Kailey Marie Mccain
USF Tampa Graduate Theses and Dissertations
Zoonotic diseases account for a significant proportion of emerging infectious diseases (EIDs), with their dynamics being heavily influenced by the environment. While the effects of the environment on population-level disease dynamics have been extensively studied, there is limited research investigating the impact of the environment on individual-level disease susceptibility and transmission. This study aims to fill this gap by creating a tool to examine the variation in immune response to Borrelia burgdorferi, the causative agent of Lyme Disease, in two competent host species, Peromyscus leucopus and Peromyscus maniculatus. Two triplex droplet digital polymerase chain reaction (ddPCR) assays were developed to …