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The Relationship Between Population Structure And Aluminum Tolerance In Cultivated Sorghum, Fernanda F. Caniato, Claudia T. Guimarães, Martha Hamblin, Claire Billot, Jean-François Rami, Barbara Hufnagel, Leon V. Kochian, Jiping Liu, Antonion Augusto F. Garcia, C. Tom Hash, Punna Ramu, Sharon Mitchell, Stephen Kresovich, Antônio Carlos Oliveira, Gisela De Avellar, Aluízio Borém, Jean-Christophe Glaszmann, Robert E. Schaffert, Jurandir V. Magalhaes

Faculty Publications

Background: Acid soils comprise up to 50% of the world’s arable lands and in these areas aluminum (Al) toxicity impairs root growth, strongly limiting crop yield. Food security is thereby compromised in many developing countries located in tropical and subtropical regions worldwide. In sorghum, SbMATE, an Al-activated citrate transporter, underlies the AltSB locus on chromosome 3 and confers Al tolerance via Al-activated root citrate release.

Methodology: Population structure was studied in 254 sorghum accessions representative of the diversity present in cultivated sorghums. Al tolerance was assessed as the degree of root growth inhibition in nutrient solution containing Al. …

The Effect Of B27 Supplement On Promoting In Vitro Propagation Of Her2/Neu-Transformed Mammary Tumorspheres, Yiben Gu, Jie Fu, Pang-Kuo Lo, Shunqi Wang, Qian Wang, Hexin Chen

Faculty Publications

A variety of human solid tumors, including breast cancer, are considered to embrace a hierarchical organization in which only rare tumor-initiating cancer stem cells are truly responsible for tumor formation. Cultivation of tumorspheres in non-adherent conditions is widely employed for enriching these putative cancer stem cells in vitro. However, the absence of a defined culture medium has handicapped further characterization and isolation of this cell population. In this study, we used mouse mammary tumor virus MMTV-HER2/neu transgenic mice that mimic the HER2/Neu-positive subtype of human breast cancer as a model system, and cultured primary tumor cells and tumorspheres from …