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Isolation And Identification Of The Toxic Principle In The Tissues Of The Ectoproct Lophopodella Carteri (Hyatt), Donald Coleman Smith
Isolation And Identification Of The Toxic Principle In The Tissues Of The Ectoproct Lophopodella Carteri (Hyatt), Donald Coleman Smith
Master's Theses
The tissue of Lophopodella carteri (Hyatt) was isolated, and the agent toxic to fish present in the tissues was extracted. A chloroform-soluble component and a water-soluble component were separated from the filtrate of the homogenized tissues. Each component was tested for toxicity. The chloroform-soluble component was found to be extremely toxic. The water-soluble component was originally non-toxic, but after several months of freezing and defrosting the tissue, this component became toxic.
The chloroform-soluble component was purified by thin-layer chromatography. Several lipid classes were obtained, of which one was toxic. This toxic class was further purified by thin-layer chromatography resulting in …