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Differentially Expressed Genes In Aortic Cells From Atherosclerosis-Resistant And Atherosclerosis-Susceptible Pigeons, Janet Lynn Anderson

Doctoral Dissertations

Representational Difference Analysis (RDA) was used to identify genes that were differentially expressed between White Carneau (WC) and Show Racer (SR) pigeon aortic smooth muscle cells. The gene(s) responsible for atherosclerotic resistance in cultured SR smooth muscle cells (SMC) were hypothesized to be silent or down regulated in the WC. In the reciprocal experiments, it was hypothesized that the gene(s) contributing to the spontaneous atherosclerotic phenotype in cultured WC SMC would not be expressed in the SR.

Total RNA was extracted from primary cultured cells of each breed, converted to cDNA, and compared in four reciprocal RDA experiments. Seventy-four transcripts …

Identification And Characterization Of Protein Phosphatase 2a Mutants In Arabidopsis Thaliana, Enhua Wang

Doctoral Dissertations

Reversible protein phosphorylation is a highly regulated process that affects almost all cellular activities. Phosphorylation involves two groups of enzymes: protein kinases and protein phosphatases. Considering the central role of these enzymes in cells, elucidating their function is extremely important. My research focuses on protein phosphatase 2A (PP2A) C subunits. PP2A is one of the most abundant types of serine/threonine phosphatases in all eukaryotic cells. Compared with animals, PP2A function is not well known in plants. Early studies using protein phosphatase inhibitors are not useful to demonstrate the function of individual PP2A subunits. A reverse genetic approach can be helpful …

The Experimental Evolution Of Host Adaptation Of The Emerging Pathogen Burkholderia Cenocepacia, Crystal Nicole Ellis

Master's Theses and Capstones

I investigated the ability of Burkholderia cenocepacia, an opportunistic bacterial pathogen, to adapt to a host. Studies have identified trade-offs associated with environmental adaptation, but few have investigated host adaptation. Consequently, I studied effects of adaptation by B. cenocepacia to onions (Allium cepa) on the ability to kill Caenorhabditis elegans. I hypothesized that adaptation to onions would reduce virulence in C. elegans. I evolved twelve populations of bacteria in onion tissue medium for 500 generations. Then, I quantified fitness differences between evolved and ancestral populations by direct competition, having developed molecular marking techniques to discriminate among competitors. Competitions revealed fitness …

A Transcriptome Comparison Of Caenorhabditis Elegans Cultured In Laboratory And Soil-Like Environments, Richard A. Roy

Master's Theses and Capstones

Caenorhabditis elegans has been the subject of numerous microarray experiments designed to help understand gene expression and function. Many such experiments have assessed the animal's transcriptional response to simple perturbations of the traditional laboratory environment.

Numerous intergenic regions showed expression. The majority were environment specific but most that were mutually expressed were structurally similar to protein-coding genes. They may well be un-annotated exons or genes. The environment specific regions were significantly shorter, overall, than coding sequences, and may represent polypeptides or non-coding RNA with regulatory or other functions.

Rapid Identification Of New England Freshwater Copepods Using A Novel Genetic Barcode, Elisah B. Allan

Master's Theses and Capstones

Identification of freshwater calanoid and cyclopoid copepods is limited to adults of certain sexes because morphological keys are mostly based on mature reproductive structures, necessitating an alternate method. Genetic barcodes are an additional tool for distinguishing species using variation in short segments of DNA. I tested the utility of the 28S rDNA D3 expansion segment as a barcode for identifying five species of calanoids and five species of cyclopoids from multiple lakes from New England. Neighbor-joining trees grouped all conspecifics together with high bootstrap support, except for Leptodiaptomus minutus. Comparisons of intra- vs. interspecific variation revealed a barcode gap for …

Use Of Genetic Tagging To Estimate Abundance And Detect Spatial Patterns Of Black Bears In New Hampshire, Stephanie Coster

Master's Theses and Capstones

Abundance estimates for black bears (Ursus americanus) are an important tool for effective management. Recent advancements in DNA technology have enabled genetic tagging mark-recapture population estimates using DNA from hair samples. I conducted a population estimate using genetic tagging in 2 study sites presumed to have different bear densities in northern New Hampshire (Pittsburg and Milan). To test repeatability, I conducted the genetic tagging estimates in 2 consecutive years. I also compared these estimates to those derived from traditional methods used by the New Hampshire Fish and Game Department (NHFG) using hunter harvest and mortality data. I found that the …