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Genetic And Molecular Analyses Of Host Symbiotic Genes And An In Vitro Regeneration System For Cicer Arietinum L, Vijayashri Ganapathy Paruvangada

Doctoral Dissertations

Ineffectively nodulating plant mutants PM405B, PM638A, and PM796B were used in molecular/genetic analyses of root nodule formation in chickpea. To establish the mode of inheritance of the mutant nodule phenotype in chickpea mutant PM638A, reciprocal crosses were made between PM638A and wild-type ICC640. The F$\sb2$ segregation data fit a 3 mutants: 1 wild-type monohybrid phenotypic ratio, indicating that ineffective nodulation is due to a monogenic dominant, nuclear mutation, tentatively designated as "Rn$\sb7$". Segregation analysis of F$\sb3$ progeny confirmed this model.

Early (ENod2) and late (Lb) nodulin cDNAs were used as heterologous probes to identify and study the expression of corresponding …

Regulation Of The Yeast Transcriptional Activator Adr1, W James Cook

Doctoral Dissertations

Glucose repression of the ADH2 gene from Saccharomyces cerevisiae is mediated by the abundance and activity of the transcriptional activator ADR1. The focus of this dissertation is to characterize the mechanisms by which glucose controls the ability of ADR1 to activate ADH2 transcription.

Glucose results in a two-fold decrease in the steady state levels of ADR1 mRNA. This glucose-dependent reduction in steady state ADR1 mRNA was shown to be due to an increased rate of ADR1 mRNA degradation. The unusually long, 510 nucleotide 5$\sp\prime$ untranslated region of the ADR1 mRNA appeared to mediate this glucose-dependent ADR1 mRNA decay.

To better …

Characterization And Inheritance Of The Fused Vein Trait In Squash (Cucurbita Pepo L), Robert Bruce Carle

Doctoral Dissertations

This investigation characterized and determined the inheritance of the fused vein trait in Cucurbita pepo L. with the purpose of evaluating its use as a rouging marker for hull-less seeded cultivars.

The trait is characterized by the partial fusion of the primary leaf veins. There is a reduction in the intraveinal leaf blade and the dorsal leaf surface appears wrinkled. Depending on genetic background, fusion begins with the fourth to tenth leaf stage and continues throughout vegetative growth. The extent of fusion also varies from slight (1-5 cm) to pronounced (15-20 cm). Anatomically, fusion results from an interruption of vein …