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University of New Hampshire

1990

Genetics

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Genetic Variability And Population Structure Of Eastern White Pine In A Two Hectare Stand, Durham, New Hampshire, Petr R. Brym Jan 1990

Genetic Variability And Population Structure Of Eastern White Pine In A Two Hectare Stand, Durham, New Hampshire, Petr R. Brym

Doctoral Dissertations

The genetic structure of a two hectare stand of eastern white pine (Pinus strobus L.) was studied by relating foliar allozyme composition of individual trees to tree location and age. Using starch gel electrophoresis, the trees were found to have variants for six of the seven enzymes analyzed. Trees of similar age and with common allozymes were found in clusters, suggesting that genetic structure existed in the stand. The genetic structure may have originated during establishment of several seedlings from common parents when openings were created in the canopy. Knowing that a genetic structure, such as that observed in this …


Structural And Functional Characterization Of The Yeast General Transcriptional Activator Ccr4, Thomas M. Malvar Jan 1990

Structural And Functional Characterization Of The Yeast General Transcriptional Activator Ccr4, Thomas M. Malvar

Doctoral Dissertations

Transcription of the glucose-repressible ADH (ADH2 locus) in Saccharomyces cerevisiae is controlled by two regulatory pathways. The general transcriptional factors CCR4, CRE1, and CRE2 constitute the first pathway while the second pathway is comprised of the trans-activators ADR1 and CCR1. Both ADR1 and CCR1 act through upstream activation sequences (UAS) found in the 5$\sp\prime$-regulatory region of the ADH2 structual gene. In contrast, the action of CCR4, CRE1, and CRE2 is likely to be at sequences near the TATAA element of ADH2.

The CCR4 locus was precisely mapped on the left arm of chromosome I where it had previously been localized. …