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Technique For Normalization Of Cross-Linked Peptide Ion Intensity To Elucidate Enzymatic Conformational Changes, Donna Hogan
Technique For Normalization Of Cross-Linked Peptide Ion Intensity To Elucidate Enzymatic Conformational Changes, Donna Hogan
Master's Theses and Capstones
Cross-linking coupled to Liquid Chromatography/Mass Spectrometry (CXMS) has become an invaluable technique for examining protein-protein interactions and monitoring enzymatic conformational changes. Thus far, comparative quantitation of cross-linked peptides measured by their mass spectrometry (MS) ion intensity is employed to deduce residue cross-linking propensity and spatial proximity. Using two distinct conformations of a structurally well-characterized model protein, we examined the correlation of cross-linked peptide MS signals and distances, but were not able to observe any obvious correlation. Conceivably, several physiochemical factors can affect residue reactivities thus MS signal intensity of the corresponding cross-linked peptides. For NHS ester chemical cross-linkers, the NHS …
Bink Domain Functional Characterization In The Regulation Of Bioluminescence In Vibrio Fischeri, Ian Ster
Bink Domain Functional Characterization In The Regulation Of Bioluminescence In Vibrio Fischeri, Ian Ster
Master's Theses and Capstones
Prokaryotes encode a remarkable ability to adapt to niches by sensing environmental cues through signal transduction systems (STSs). Typical STS proteins interact through a phosphorylation relay between histidine (His) and aspartate (Asp) residues within modular domains on sensory kinase and response regulator (RR) proteins to elicit cellular responses. A single point mutation in the sensor kinase BinK (BinK1 R537C) conferred an outstanding ability for the non-native V. fischeri strain MJ11 to successfully colonize Euprymna scolopes by affecting multiple symbiotic phenotypes including luminescence activation. However, the role of BinK in luminescence, the interacting partners, and functional mechanism are unknown. We hypothesized …