Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Entire DC Network
Genomic Resource Development For A Diploid Mint: Mentha Longifolia, Zahra Hadadian
Genomic Resource Development For A Diploid Mint: Mentha Longifolia, Zahra Hadadian
Master's Theses and Capstones
This research project aimed to develop genomic resources needed to enable construction of a genetic linkage map of the diploid mint species Mentha longifolia. Such a map would facilitate identification of plant genes involved in resistance to Verticillium fungal infection. For this purpose, a small genomic library was constructed from germplasm accession CMEN 585, 279 genomic inserts were sequenced and annotated and 19 PCR primer pairs were designed and tested on two resistant and two susceptible accessions. The Cleaved Modified Polymorphic Sequence (CAPS) method of molecular marker genotyping was found to detect little variation between crossing parents CMEN 585 (resistant) …
Microbial Interactions With Oysters From The Great Bay Estuary: Characterization Of Endemic Vibrio Choleraeand Oyster Metagenetics, Brian Michael Schuster
Microbial Interactions With Oysters From The Great Bay Estuary: Characterization Of Endemic Vibrio Choleraeand Oyster Metagenetics, Brian Michael Schuster
Master's Theses and Capstones
Within the last two decades the number of gastroenteritis seafood-borne outbreaks due to Vibrio species has increased. These infections are usually caused by the consumption of raw or undercooked shellfish. I took two approaches to describe the ecology of Vibrios in the Great Bay Estuary, Durham, New Hampshire. First, I performed a phenotypic and multi-locus sequence analysis of 31 V. cholerae strains isolated from oysters, water and sediment in 2008 and 2009. I concluded that the V. cholerae population is endemic, diverse and highly recombinatory, but none of the isolates contain the virulence markers indicative of clinical strains. Next, I …
Regulation Of Photoreceptor Phosphodiesterase By Prenyl Binding Protein And By Other Interacting Proteins In Photoreceptor Cells, Hannah J. Gitschier
Regulation Of Photoreceptor Phosphodiesterase By Prenyl Binding Protein And By Other Interacting Proteins In Photoreceptor Cells, Hannah J. Gitschier
Master's Theses and Capstones
Photoreceptor phosphodiesterase (PDE6) is a central component of the visual pathway. While initial PDE6 activation following light stimulation of photoreceptors is well understood, regulation of PDE6 during recovery and light adaptation may require additional components that interact with PDE6. Two approaches were taken to characterize these potential interactions. The first explored the interaction of PDE6 with prenyl binding protein (PrBP/delta) and demonstrated that changes in membrane localization of PDE6 may occur during light adaptation as a result of association with PrBP/delta. The second approach characterized the PDE6 "interactome" by a mass spectrometric identification of 75 proteins that co-purify with PDE6 …
Truncated Mortalin In Animal Cancer, Katrina K. Olson
Truncated Mortalin In Animal Cancer, Katrina K. Olson
Master's Theses and Capstones
Hematopoetic neoplasia or clam hemocyte cancer (a leukemia-like disease) has been studied in a number of bivalve molluscs for the last 20 years. Recent molecular studies of the hemocytes of the soft shell clam, Mya arenaria, have demonstrated an interaction between p53 and mortalin, the mitochondrial Hsp70. The former protein is intimately involved in the initiation of cell-cycle arrest, apoptosis, DNA repair, and cell differentiation. In cancerous clams, wild-type p53 is sequestered in hemocyte cytoplasm by mortalin and cannot be translocated to the nucleus. This is critical because although p53 is functions properly, it is unable to enter the nucleus …
Using Pentaploids As Tools For Studying Genomic Composition And Allele Transmission Patterns In Octoploid Fragaria, Elizabeth Poulsen
Using Pentaploids As Tools For Studying Genomic Composition And Allele Transmission Patterns In Octoploid Fragaria, Elizabeth Poulsen
Master's Theses and Capstones
A unique pentaploid population of Fragaria (strawberry) was developed to gain insight into octoploid genome composition and allele transmission patterns. This population was produced by crossing representatives of two divergent subspecies of octoploid F. virginiana and then by crossing an octoploid hybrid plant with diploid F. vesca. To enable examination of allele transmission patterns, the intergenic region of the gRGA1-Subtilase gene pair locus was amplified, and PCR products were cloned, sequenced, and compared to define the allele composition of the hybrid octoploid. The sequencing data revealed three distinct major haplotypes and additional subtypes. The pentaploids were genotyped utilizing the FEL-CAPS …