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Functional Analysis Of The Caf1 Protein, Takbum Ohn

Doctoral Dissertations

The CAF1 protein is a component of the CCR4-NOT deadenylase complex. While yeast CAF1 displays deadenylase activity, this activity is not required for its function in vivo, and CCR4 is the primary deadenylase in the complex. In order to identify CAF1-specific functional regions required for deadenylation in vivo, we targeted for mutagenesis six regions of CAF1 that are specifically conserved among CAF1 orthologs. Defects in three regions of the CAF1 protein (residues 173-175, residues 255-257 and residues 340-342, alleles caf1-1, caf1-3 and caf1-6, respectively) were found to dramatically reduce the rate of deadenylation in vivo and to result in typical …

Analysis Of The Role Of Poly(A) -Binding Protein (Pab1) In The Mrna Degradation Process In Yeast, Gang Yao

Doctoral Dissertations

The mRNA deadenylation process influences multiple aspects of protein synthesis and is known to be the major factor controlling mRNA decay rates. My data demonstrates that yeast PAB1 plays both positive and negative roles in controlling deadenylation, and I have identified particular regions of PAB1 involved in controlling different aspects of the mRNA degradative process. I have found that yeast PAB1 does not play a simple, obstructionist role in regulating CCR4 deadenylation. Instead, PAB1-PAB1 protein interactions, as mediated by the PAB1 proline-rich region (P domain) and the RRM1 domain, are required for the CCR4 deadenylase activity. The P and RRM1 …

A Molecular And Morphological Investigation Of The Red Seaweed Genus Porphyra (Bangiales, Rhodophyta) In The Northwest Atlantic, Troy Lee Bray

Doctoral Dissertations

To evaluate the possible occurrence of cryptic Porphyra taxa in the Northwest Atlantic, extensive field collections were made during winter and spring periods when relatively few previous collections had been made. Approximately 100 different sites extending from Chance Harbor, New Brunswick, Canada to Rye, New York, USA, were sampled during multiple years (2001-2005). Historical specimens from several herbaria (NHA, FH, BM, WTU, MICH, US) were also examined for possible cryptic taxa. A combination of morphological and molecular tools was used to screen both recent and historical collections.

Sequences from the plastid-encoded, ribulose-1, 5-bisphosphate carboxylase-oxygenase large subunit (rbcL) gene and rbcL- …

Studies Of Flower Color In Anagallis Monelli L, Andrea Quintana Pinilla

Master's Theses and Capstones

One of the main goals for plant breeders in the floriculture industry is to develop plants with new attributes. Characteristics such as flower size, growth habit, resistance to pests and diseases, flower size, and particularly flower color are among the most important traits to breed and improve. One of the best ways to make a plant popular in the market is to offer a wide variety of flower colors. A. monelli is an ornamental plant used for hanging baskets and as a bedding plant, and until recently, cultivars available were only blue- or orange-flowered. Recent breeding research on this species …

The Development And Utilization Of Est (Expressed Sequence Tag) Resources In The Diploid Strawberry Model System, Robin Leigh Brese

Master's Theses and Capstones

Enhancement of genomic resources is needed for the cultivated octoploid strawberry Fragaria x ananassa (2n=8x=56). A diploid (2n=2x=14) relative, Fragaria vesca, has been used as a model system for strawberry genomics because of its small (~200 Mb) basic genome size, ancestry to the octoploid strawberries and availability of inbred lines, among other favorable features.

In this study, a cDNA library was constructed from developing flower buds of Fragaria vesca ssp. vesca cv. 'Yellow Wonder'. A cDNA library of 7680 clones was created using the CLONTECH SMART cDNA Library Construction kit. Sequencing and bioinformatic analyses of 3298 clones resulted in 2717 …

A Test Of The Nutrient-Productivity Model In The Gulf Of Maine Using The Intertidal Mussel Mytilus Edulis, Meredith A. Bailey

Master's Theses and Capstones

Historical concepts of top-down control (predator-prey interactions) on rocky intertidal community structure have transitioned to studies on bottom-up effects (nutrient supply and larval transport) as significant factors affecting rocky intertidal community structure. Studies performed on rocky intertidal locations along the Gulf of Maine (GOM) at multiple sites and seasons in 2004 and 2006 examined the ecology of Mytilus edulis populations by measuring size frequency distributions, diet quality (stable isotope composition) and physiological performance of individuals using condition indices and RNA/DNA ratios. Data were correlated to satellite imagery for sea surface temperature and chlorophyll a concentration and individuals were genetically tested …

Calcium-Dependent Protein Kinases In Physcomitrella Patens, Kathryn Chervincky

Master's Theses and Capstones

Calcium-dependent protein kinases (CDPKs) are a group of plant serine-threonine kinases that are stimulated in response to increases in intracellular calcium concentrations. Physcomitrella patens is a moss and a member of the bryophyte family. Physcomitrella has gained popularity as a model research organism due to the simple morphology of the plant as well as many other desirable characteristics. My research utilized Physcomitrella to accomplish four goals. The first goal was to identify and obtain sequence for as many CDPKs as possible in Physcomitrella. The second goal was to confirm the calcium-activated protein kinase activity of the identified CDPKs. The third …

Mhc (B) Complex Recombinant Immune Response, Nicole Gay Wilkinson

Master's Theses and Capstones

Recombination between chicken major histocompatibility complex (MHC) class I B-F and class IV B-G genes has enabled more precise identification of genes controlling immune responses. Congenic lines, each containing a unique MHC recombinant, were developed with 99.9% genetic uniformity to inbred Line UCD 003 (B17B17). A new recombinant, R13 (BF17-BG23), originated in the tenth backcross generation for R1 (BF24-BG23). Two experiments tested the R13 immune response to bovine red blood .cells (BRBC) and Rous sarcoma virus (RSV).

A single R13B17 sire mated to five R131317 dams produced progeny segregating for R13R13, R13B17, and B17B17 genotypes. These progeny were injected with …