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Nitrogen Fixation Mutants Of The Actinobacterium Frankia Casuarinae Cci3, Ken-Ichi Kucho, Daiki Tamari, Shintaro Matsuyama, Takeshi Nabekura, Louis S. Tisa Dec 2017

Nitrogen Fixation Mutants Of The Actinobacterium Frankia Casuarinae Cci3, Ken-Ichi Kucho, Daiki Tamari, Shintaro Matsuyama, Takeshi Nabekura, Louis S. Tisa

Molecular, Cellular & Biomedical Sciences

Frankia is a representative genus of nitrogen-fixing (N2-fixing) actinobacteria; however, the molecular mechanisms underlying various phenomena such as the differentiation of a N2 fixation-specific structure (vesicle) and the regulation of N2 fixation (nif) genes, have yet to be elucidated in detail. In the present study, we screened hyphal fragments of Frankia casuarinae that were mutagenized by 1-methyl-3-nitro-1-nitrosoguanidine or gamma rays, and isolated 49 candidate N2 fixation mutants. Twelve of these mutants were selected for further study, and their abilities to grow in NH3-deficient (N-) liquid media and their rates of acetylene reduction activities were evaluated. Eleven mutant strains were confirmed …


Comparative Mapping Of Arabidopsis Thaliana And Brassica Oleracea Chromosomes Reveals Islands Of Conserved Organization, Stanley P. Kowalski, Tien-Hung Lan, Kenneth A. Feldmann, Andrew H. Patterson Jan 1994

Comparative Mapping Of Arabidopsis Thaliana And Brassica Oleracea Chromosomes Reveals Islands Of Conserved Organization, Stanley P. Kowalski, Tien-Hung Lan, Kenneth A. Feldmann, Andrew H. Patterson

Law Faculty Scholarship

The chromosomes of Arabidopsis thaliana and Brassica oleracea have been extensively rearranged since the divergence of these species; however, conserved regions are evident. Eleven regions of conserved organization were detected, ranging from 3.7 to 49.6 cM in A. thaliana, spanning 158.2 cM (24.6%) of the A. thaliana genome, and 245 cM (29.9%) of the B. oleracea genome. At least 17 translocations and 9 inversions distinguish the genomes of A. thaliana and B. oleracea. In one case B. oleracea homoeologs show a common marker order, which is distinguished from the A. thaliana order by a rearrangement, indicating that the lineages of …