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Sensors Based On Immobilized Fluorogenic Reagents, Linda Anne Saari
Sensors Based On Immobilized Fluorogenic Reagents, Linda Anne Saari
Doctoral Dissertations
Sensors based on the fluorescence of immobilized fluorogenic reagents are prepared and their characteristics are defined. The fluorogenic reagent is chemically immobilized on a solid support and attached to the common end of a bifurcated fiber optic bundle. Fluorescence is excited through one arm of the fiber optic and observed through the other arm. The fluorogenic reagents fluoresceinamine, morin, calcein, and quinalizarin are immobilized on powdered cellulose via cyanuric chloride.
Consistent with previous studies of the pH dependence of fluorescein, the fluorescence from the immobilized fluoresceinamine sensor increases with pH. Immobilized fluoresceinamine serves as a viable pH sensor, but can …
The Determination Of Stability Constants And Complexing Capacities Of Natural Organic Matter For Copper (2+), Cobalt (2+) And Manganese (2+) By Fluorescence Quenching Titration, David K. Ryan
Doctoral Dissertations
The purpose of this study is to develop methodologies for the investigation of the metal ion binding properties of natural humic substances. Humic materials are prevalent in soil and water and their important metal ion detoxification and transport properties are the major justification for this work. The primary emphasis is the determination of the complexing capacity of a sample for a particular metal ion under fixed conditions.
The initial part of the investigation involves the application of chelating ion exchange to remove and quantify humic-chelated trace metal ions from water samples. Amine, dithiocarbamate and 8-hydroxyquinoline chelating agents immobilized on porous …
The Study And Characterization Of A New Procedure For Applying Carbide Coatings To Graphite Furnaces Used In Atomic Absorption Spectroscopy, Manuel Charles Almeida
The Study And Characterization Of A New Procedure For Applying Carbide Coatings To Graphite Furnaces Used In Atomic Absorption Spectroscopy, Manuel Charles Almeida
Doctoral Dissertations
The object of this dissertation is the study and characterization of a new method for applying carbide coatings to graphite furnaces used in atomic absorption spectroscopy. The proposed method consists of impregnating graphite furnaces, under reduced pressure, with a liquid metal chloride. After soaking in water to hydrolyze the metal chloride to metal oxide, the furnace is fired at high temperature to form the carbide. Three metal chlorides, TiCl(,4), MoCl(,5) and WCl(,6), were used to apply TiC, MoC and WC coatings, respectively. Experimental details of the coating procedures are given.
Characterization of the various coatings was carried out using ESCA, …
Flow Systems For Clinical Analysis Using Immobilized Enzymes And Chemiluminescence Detection (Chromium, Glucose), Jerome L. Mullin
Flow Systems For Clinical Analysis Using Immobilized Enzymes And Chemiluminescence Detection (Chromium, Glucose), Jerome L. Mullin
Doctoral Dissertations
Analytical systems used in a clinical setting should offer high sample throughput and eae of operation, while at the same time providing selective, sensitive, accurate and precise determinations of clinically important analytes. Described in this study are two flow systems based on chemiluminescence (CL) detection that provide the features important in a practical clinical method: a flow injection analysis (FIA) system and a two phase flow system. Both systems take advantage of the specificity of enzyme reactions.
FIA systems for the determination of Cr(III) and glucose, based on luminol CL were developed. The Cr(III) system used EDTA as a masking …