Digital Commons Network^™

Habitat Heterogeneity In Nebraska Streams And Distribution Prediction For Tier-1 Cyprinids Using Multi-Scale Modeling Of Fluvial And Landscape Features, Connor P. Hart

School of Natural Resources: Dissertations, Theses, and Student Research

Multiscale environmental processes determine in-stream habitat conditions which drive species distributions. Habitat constitutes the physical template upon which ecological processes occur and species conduct life stage activities. Habitat heterogeneity promotes biodiversity of aquatic systems. Stream classification informs freshwater conservation by providing a useful framework to account for habitat heterogeneity, often based on landscape regions of similar environmental processes. A greater understanding of landscape-based classification frameworks as means to classify stream systems may improve understanding of drivers of biodiversity. Using Nebraska as a case study, on a statewide scale, objectives were 1) to characterize habitat availability for several at-risk fish species, …

Slow-Release Lanthanum Effectively Reduces Phosphate In Eutrophic Ponds Without Accumulating In Fish, C. Mccright, J. Mccoy, N. Robbins, S. Comfort

School of Natural Resources: Faculty Publications

No abstract provided.

Larval Fish Abundance In The Benthic And Surface Drift Of The Missouri River, Ryan L. Ruskamp

Department of Agricultural Leadership, Education and Communication: Dissertations, Theses, and Student Scholarship

Knowledge of the larval fish community of the Missouri River is one of the biggest gaps in fisheries research. The Nebraska Game and Parks Commission has sampled the drift of the Missouri River for many years (1983-2015), but these data have not been compiled into a unified assessment. Therefore, the objectives of this study were to: 1) quantify temporal and spatial aspects of larval fish community composition (richness) and structure (abundance) of the surface drift, 2) quantify associations of larval fish communities of the drift to different discharges of the Missouri River, 3) quantify the larval benthic drift community, and …

Fingerlings Mass Estimation: A Comparison Between Deep And Shallow Learning Algorithms, Adair Da Silva Oliveira Junior, Diego André Sant’Ana, Marcio Carneiro Brito Pache, Vanir Garcia, Vanessa Aparecida De Moares Weber, Gilberto Astolfi, Fabricio De Lima Weber, Geazy Vilharva Menezes, Gabriel Kirsten Menezes, Pedro Lucas França Albuquerque, Celso Soares Costa, Eduardo Quirino Arguelho De Queiroz, João Victor Araújo Rozales, Milena Wolff Ferreira, Marco Hiroshi Naka, Hemerson Pistori

School of Computing: Faculty Publications

The paper presents some results regarding the automatic mass estimation of Pintado Real fingerlings, using machine learning techniques to support the fish production process. For this purpose, an image dataset called FISHCV1206FSEG, was created which is composed of 1206 images of fingerlings with their respective annotated masses. Through the fish contours, the area and perimeter were extracted, and submitted to the J48, SVM, and KNN classification algorithms and a linear regression algorithm. The images were also submitted to ResNet50, In- ceptionV3, Exception, VGG16, and VGG19 convolutional neural networks. As a result, the classification algorithm J48 reached an accuracy of 58.2% …

Protocol For Monitoring Fish Communities In Small Streams In The Heartland Inventory And Monitoring Network, Version 2.0, Hope R. Dodd, David G. Peitz, Gareth Rowell, Janice A. Hinsey, David E. Bowles, Lloyd W. Morrison, Michael D. Debacker, Jennifer L. Haack-Gaynor, Jeffrey M. Williams

United States National Park Service: Publications

Executive Summary

Fish communities are an important component of aquatic systems and are good bioindicators of ecosystem health. Land use changes in the Midwest have caused sedimentation, erosion, and nutrient loading that degrades and fragments habitat and impairs water quality. Because most small wadeable streams in the Heartland Inventory and Monitoring Network (HTLN) have a relatively small area of their watersheds located within park boundaries, these streams are at risk of degradation due to adjacent land use practices and other anthropogenic disturbances. Shifts in the physical and chemical properties of aquatic systems have a dramatic effect on the biotic community. …

Blood Parasites Of Fish Of The Baikal Basin, T. R. Khamnueva, Darima R. Baldanova

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

Eight species of trypanosomes were found in nine species of fish in the Lake Baikal: Trypanosoma percae, T. amurensis, T. schulmani, T. dogieli, T. magna, T. sp.1, T. sp.2. Five species of trypanoplasms were detected in the blood of 13 species of Baikal fish: Trypanoplasma lomakini, T. littoralis, T. cotti, T. zaikai, T. sp. In the erythrocytes of the sand sculpin, Leocottus kesslerii were observed haemogregarins.

Flow Alteration-Ecology Relationships In Ozark Highland Streams: Consequences For Fish, Crayfish And Macroinvertebrate Assemblages, Dustin T. Lynch, Douglas R. Easure, Daniel D. Magoulick

United States Geological Survey: Staff Publications

We examined flowalteration-ecology relationships in benthic macroinvertebrate, fish, and crayfish assemblages in Ozark Highland streams, USA, over two years with contrasting environmental conditions, a drought year (2012) and a flood year (2013). We hypothesized that: 1) there would be temporal variation in flow alteration-ecology relationships between the two years, 2) flow alteration-ecology relationshipswould be stronger during the drought year vs the flood year, and 3) fish assemblages would show the strongest relationships with flow alteration. We used a quantitative richest-targeted habitat (RTH) method and a qualitative multihabitat (QMH) method to collect macroinvertebrates at 16 USGS gaged sites during both years. …

Flow Alteration-Ecology Relationships In Ozark Highland Streams: Consequences For Fish, Crayfish And Macroinvertebrate Assemblages, Dustin Thomas Lynch, Douglas R. Leasure, Daniel D. Magoulick

United States Geological Survey: Staff Publications

We examined flowalteration-ecology relationships in benthic macroinvertebrate, fish, and crayfish assemblages in Ozark Highland streams, USA, over two years with contrasting environmental conditions, a drought year (2012) and a flood year (2013). We hypothesized that: 1) there would be temporal variation in flow alteration-ecology relationships between the two years, 2) flow alteration-ecology relationshipswould be stronger during the drought year vs the flood year, and 3) fish assemblages would show the strongest relationships with flow alteration. We used a quantitative richest-targeted habitat (RTH) method and a qualitative multihabitat (QMH) method to collect macroinvertebrates at 16 USGS gaged sites during both years. …

Experimental Assessment Of Predation Risk For Juvenile Green Sturgeon, Acipenser Medirostris, By Two Predatory Fishes, S. Baird, A. E. Steel, D. E. Cocherell, Jamilynn B. Poletto, R. Follenfant, N. A. Fangue

School of Natural Resources: Faculty Publications

No abstract provided.

Novel Information On The Morphology, Phylogeny And Distribution Of Camallanid Mematodes From Marine And Freshwater Hosts In South Africa, Including The Description Of Camallanus Sodwanaensis N. Sp., Roman Svitin, Marliese Truter, Olena Kudlai, Nico J. Smit, Louis Du Preez

Harold W. Manter Laboratory: Library Materials

Four species of previously known nematodes from the family Camallanidae were found from different hosts in South Africa: Batrachocamallanus xenopodis from the frog Xenopus muelleri, Paracamallanus cyathopharynx, and Procamallanus pseudolaeviconchus from the catfish Clarias gariepinus and Spirocamallanus daleneae from the catfish Synodontis zambezensis. In the material collected from various marine fishes, several specimens of nematodes from the genus Camallanus clearly differed from all previously known species. Based on morphological differences these specimens are assigned to a new species, C. sodwanaensis. Molecular data of 18S and 28S rDNA and COI sequences are provided for the collected species …

Fish And Macroinvertebrate Response To Restored Off Channel Habitats On The Lower Platte River, Nebraska, Caleb Uerling

School of Natural Resources: Dissertations, Theses, and Student Research

Anthropogenic alterations to large rivers ranging from impoundments to levees have caused many rivers to no longer access the floodplain. The ecological integrity of floodplain rivers depends on the interaction between main-channel and floodplain habitats. Fish communities inhabiting floodplain habitats are often dictated by the type of habitat and conditions within that habitat. As restoration projects are undertaken it is imperative that managers understand how fish and macroinvertebrates respond to these events. We collected fish, macroinvertebrates, and habitat parameters on two restored floodplain habitats on the lower Platte River, Nebraska to answer questions about aquatic community response to floodplain restoration …

Hydraulics Near Unscreened Diversion Pipes In Open Channels: Large Flume Experiments, Ali Ercan, M. Levent Kavvas, Kara Carr, Zachary Hockett, Houssein Bandeh, Timothy D. Mussen, Dennis E. Cocherell, Jamilynn B. Poletto, Joseph J. Cech Jr., Nann A. Fangue

School of Natural Resources: Faculty Publications

Most of the water diversions on the Sacramento and San Joaquin Rivers (California, United States) and their tributaries are currently unscreened. These unscreened diversions are commonly used for irrigation and are potentially harmful to migrating and resident fishes. A large flume (test section: 18.29 m long, 3.05 m wide and 3.20 m high) was used to investigate the hydraulic fields near an unscreened water diversion under ecologically and hydraulically relevant diversion rates and channel flow characteristics. We investigated all combinations of three diversion rates (0.28, 0.42, and 0.57 m³/s) and three sweeping velocities (0.15, 0.38, and 0.61 m/s), …

Dietary Cation–Anion Difference May Explain Why Ammonium Urate Nephrolithiasis Occurs More Frequently In Common Bottlenose Dolphins (Tursiops Truncatus) Under Human Care Than In Free-Ranging Common Bottlenose Dolphins1, A. J. Ardente, R. S. Wells, C. R. Smith, M. T. Walsh, E. D. Jensen, T. L. Schmitt, J. Colee, B. J. Vagt, R. C. Hill

U.S. Navy Research

Ammonium urate nephrolithiasis frequently develops in common bottlenose dolphins (Tursiops truncatus) managed under human care but is rare in free-ranging common bottlenose dolphins. In other species, the dietary cation–anion difference (DCAD) can affect ammonium urate urolith formation by increasing proton excretion as ammonium ions. Therefore, differences in diet between the 2 dolphin populations could affect urolith formation, but the DCAD of most species consumed by free-ranging and managed dolphins is unknown. To compare the nutrient composition of diets consumed by free-ranging and managed bottlenose dolphins, samples (n = 5) of the 8 species of fish commonly consumed …

Unusual Aerobic Performance At High Temperatures In Juvenile Chinook Salmon, Oncorhynchus Tshawytscha, Jamilynn B. Poletto, Dennis E. Cocherell, Sarah E. Baird, Trinh X. Nguyen, Valentina Cabrera-Stagno, Anthony P. Farrell, Nann A. Fangue

School of Natural Resources: Faculty Publications

Understanding how the current warming trends affect fish populations is crucial for effective conservation and management. To help define suitable thermal habitat for juvenile Chinook salmon, the thermal performance of juvenile Chinook salmon acclimated to either 15 or 19°C was tested across a range of environmentally relevant acute temperature changes (from 12 to 26°C). Swim tunnel respirometers were used to measure routine oxygen uptake as a measure of routine metabolic rate (RMR) and oxygen uptake when swimming maximally as a measure of maximal metabolic rate (MMR) at each test temperature. We estimated absolute aerobic scope (AAS = MMR − RMR), …

The Nebraska Stream Surveys--The Photographs: Nebraska Streams Photographed During Raymond Johnson's Statewide Stream Fishery Survey (1939-41) And How They Look Now, 78 Years Later., Steven C. Schainost

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

Raymond E. Johnson and associates surveyed Nebraska's streams for fish (1939-1941). This print-ready pdf shows original photographs from the survey side-by-side with the author's recent photographs from the same or nearby locations, along with comments regarding changes observed. Includes map and index of locations.

The Nebraska Stream Fishery Surveys: Being A Comparison Of The First Statewide Collections Of Raymond Johnson (1939-1941) With A Recent Resurvey (2003-2005), Steven C. Schainost, Edward J. Peters

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The first statewide survey of Nebraska fishes was done between 1939 and 1941. This survey was repeated between 2003 and 2005. This document compares and contrasts the results of those two surveys to illustrate what has changed and what hasn't changed over that 60 year period. It is hoped that this document can serve as a resource for future researchers to track changes in Nebraska's fish communities.

Dietary Cation–Anion Difference May Explain Why Ammonium Urate Nephrolithiasis Occurs More Frequently In Common Bottlenose Dolphins (Tursiops Truncatus) Under Human Care Than In Free-Ranging Common Bottlenose Dolphins, A. J. Ardente, R. S. Wells, C. R. Smith, M. T. Walsh, E. D. Jensen, T. L. Schmitt, J. Colee, B. J. Vagt, R. C. Hill

U.S. Navy Research

Ammonium urate nephrolithiasis frequently develops in common bottlenose dolphins (Tursiops truncatus) managed under human care but is rare in free-ranging common bottlenose dolphins. In other species, the dietary cation–anion difference (DCAD) can affect ammonium urate urolith forma- tion by increasing proton excretion as ammonium ions. Therefore, differences in diet between the 2 dolphin populations could affect urolith formation, but the DCAD of most species consumed by free-ranging and managed dolphins is unknown. To compare the nutrient composition of diets consumed by free-ranging and managed bottlenose dolphins, samples (n = 5) of the 8 species of fish commonly …

An Ecotoxicological Survey Of Tributaries Of The Selenge River, Mongolia, August 2010, Viktor T. Komov, Ch. Javzan, William G. Brumbaugh

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

The biodiversity of the Selenga river basin and the receiving waters of the lake Baikal, are among the greatest in the world with over 1,700 known endemic species of plants and animals. Mining activities along the Selenga river and its tributaries pose a major threat of chemical contamination, potentially reducing habitat quality and suitability for aquatic species. Moreover, the Selenga river serves as a major water source for the lake Baikal. Little information exists on the chemical contaminant concentrations in the Selenga river basin. Thus, the objectives of our study were to evaluate the concentrations of metals in soil, sediment, …

The Status Of Fishes In The Missouri River, Nebraska: Pallid Sturgeon (Scaphirhynchus Albus), Kirk D. Steffensen, Dane A. Shuman, Robert A. Klumb, Sam Stukel

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Anthropogenic alterations to the Missouri River have placed the Pallid Sturgeon (Scaphirhynchus albus) population in jeopardy and contributed to their listing as an endangered species. Pallid sturgeon were always less common than the sympatric Shovelnose Sturgeon (S. platorynchus); however, Pallid Sturgeon seemed to be more affected by river alterations as the river sturgeon ratio has become more skewed towards Shovelnose Sturgeon. Shortly after listing, population augmentation with hatchery produced Pallid Sturgeon began to supplement the diminishing wild population. Therefore, the objective of this study was to present the current population status of the Pallid Sturgeon in …

Co-Occurrence Of The Cyanotoxins Bmaa, Daba And Anatoxin-A In Nebraska Reservoirs, Fish, And Aquatic Plants, Maitham Ahmed Al-Sammak, Kyle D. Hoagland, David A. Cassada, Daniel D. Snow

Nebraska Water Center: Faculty Publications

Several groups of microorganisms are capable of producing toxins in aquatic environments. Cyanobacteria are prevalent blue green algae in freshwater systems, and many species produce cyanotoxins which include a variety of chemical irritants, hepatotoxins and neurotoxins. Production and occurrence of potent neurotoxic cyanotoxins β-N-methylamino-L-alanine (BMAA), 2,4-diaminobutyric acid dihydrochloride (DABA), and anatoxin-a are especially critical with environmental implications to public and animal health. Biomagnification, though not well understood in aquatic systems, is potentially relevant to both human and animal health effects. Because little is known regarding their presence in fresh water, we investigated the occurrence and potential for bioaccumulation of cyanotoxins …

An Environmental Oestrogen Disrupts Fish Population Dynamics Through Direct And Transgenerational Effects On Survival And Fecundity, Adam Scwindt, Dana L. Winkelman, Kristen Keteles, Mark Murphy, Alan M. Vajda

United States Environmental Protection Agency: Staff Publications

1. Increased need for water and projected declines in precipitation due to climate change could leave waterways increasingly dominated by wastewater effluent. Understanding how components of wastewater influence fish populations is necessary for effective conservation and management. Despite research demonstrating effects of oestrogens, such as 17α-ethynylestradiol (EE2), on fish physiology and population failure, the generality of population responses is uncertain and the underlying mechanisms affecting population declines are unknown. EE2 is the steroid oestrogen in human contraceptive pills and has been measured up to 11 ng L^-1in the environment. 2. We identify disrupted population dynamics due to direct …

An Environmental Oestrogen Disrupts Fish Population Dynamics Through Direct And Transgenerational Effects On Survival And Fecundity, Adam R. Schwindt, Dana L. Wrinkelman, Kristen Keteles, Mark Murphy, Alan M. Vajda

United States Environmental Protection Agency: Staff Publications

1. Increased need for water and projected declines in precipitation due to climate change could leave waterways increasingly dominated by wastewater effluent. Understanding how components of wastewater influence fish populations is necessary for effective conservation and management. Despite research demonstrating effects of oestrogens, such as 17a-ethynylestradiol (EE2), on fish physiology and population failure, the generality of population responses is uncertain and the underlying mechanisms affecting population declines are unknown. EE2 is the steroid oestrogen in human contraceptive pills and has been measured up to 11 ng L ^-1 in the environment.

2. We identify disrupted population dynamics due to direct …

Efficacy Of A Sensory Deterrent And Pipe Modifications In Decreasing Entrainment Of Juvenile Green Sturgeon (Acipenser Medirostris) At Unscreened Water Diversions, Jamilynn B. Poletto, Dennis E. Cocherell, Timothy D. Mussen, Ali Ercan, Hossein Bandeh, M. Levent Kavvas, Joseph J. Cech Jr., Nann A. Fangue

School of Natural Resources: Faculty Publications

Water projects designed to extract fresh water for local urban, industrial and agricultural use throughout rivers and estuaries worldwide have contributed to the fragmentation and degradation of suitable habitat for native fishes. The number of water diversions located throughout the Sacramento–San Joaquin watershed in California’s Central Valley exceeds 3300, and the majority of these are unscreened. Many anadromous fish species are susceptible to entrainment into these diversions, potentially impacting population numbers. In the laboratory, juvenile green sturgeon (Acipenser medirostris) have been shown to have high entrainment rates into unscreened diversions compared with those of other native California fish …

Fitness In Animals Correlates With Proximity To Discontinuities In Body Mass Distributions, David G. Angeler, Craig R. Allen, Anna Vila-Gispert, David Almeida

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Discontinuous structure in landscapes may cause discontinuous, aggregated species body-mass patterns, reflecting the scales of structure available to animal communities within a landscape. Empirical analyses have shown that the location of species within body mass aggregations, which reflect this scalespecific organization, is non-random with regard to several ecological phenomena, including species extinctions. The propensity of declining species to have body masses proximate to discontinuities suggests that transition zones between scaling regimes ultimately decreases the ecological fitness for some species. We test this proposition using vulnerable and unthreatened fish species in Mediterranean streams with differing levels of human impact. We show …

Tissue-Specific Molecular Immune Response To Lipopolysaccharide Challenge In Emaciated Anadromous Arctic Charr, Mathilakath Vijayan, Anju Philip, Even Jørgensen, Alec Maule

United States Geological Survey: Staff Publications

Anadromous Arctic charr (Salvelinus alpinus) undergo voluntary winter fasting for months in the Arctic. We tested the hypothesis that extended fasting will compromise the ability of this species to evoke an immune response. Charr were either fed or fasted for 85 days and challenged with lipopolysaccharide (LPS), and the molecular immune response in the liver and spleen assessed at 8 and 96 h post-injection. LPS increased IL-1β, IL-8, and serum amyloid protein A (SAA) mRNA levels in both groups, but the liver IL- 1β and IL-8, and spleen IL-8 responses were reduced in the fasted group. Fasting upregulated SOCS-1 and …

Some Aspects Of The Biology Of Monogenean (Platyhelminth) Parasites Of Marine And Freshwater Fishes, Graham C. Kearn

Harold W. Manter Laboratory: Library Materials

Müller was the first to describe a monogenean, collected from the skin of the halibut (Hippoglossus hippoglossus). However, he regarded the parasite as a leech and named it Hirudo hippoglossi. It was not until 1858 that its status as a monogenean was established by van Beneden and named Epibdella (now Entobdella) hippoglossi. Van Beneden published a detailed and accurate description of the parasite and one of his excellent illustrations is reproduced here. Entobdella hippoglossi is one of the largest monogeneans, measuring up to 2 cm in length. It has a smaller relative, measuring 5 to …

Review Of The Negative Influences Of Non-Native Salmonids On Native Fish Species, Kelly C. Turek, Mark A. Pegg, Kevin L. Pope

Great Plains Research: A Journal of Natural and Social Sciences

Non-native salmonids are often introduced into areas containing species of concern, yet a comprehensive overview of the short- and long-term consequences of these introductions is lacking in the Great Plains. Several authors have suggested that non-native salmonids negatively influence species of concern. The objective of this paper is to review known interactions between non-native salmonids and native fishes, with a focus on native species of concern. After an extensive search of the literature, it appears that in many cases non-native salmonids do negatively influence species of concern (e.g., reduce abundance and alter behavior) via different mechanisms (e.g., predation and competition). …

What Are The Toxicological Effects Of Mercury In Arctic Biota?, Rune Dietz, Christian Sonne, Niladri Basu, Birgit Braune, Todd O'Hara, Robert J. Letcher, Tony Scheuhammer, Magnus Andersen, Claus Andreasen, Dennis Andriashek, Gert Asmund, Aurore Aubail, Hans Baagøe, Erik W. Born, Hing M. Chan, Andrew E. Derocher, Philippe Grandjean, Katrina Knott, Maja Kirkegaard, Anke Krey, Nick Lunn, Francoise Messier, Marty Obbard, Morten T. Olsen, Sonja Ostertag, Elizabeth Peacock, Aristeo Renzoni, Frank F. Rigét, Janneche Utne Skaare, Gary Stern, Ian Stirling, Mitch Taylor, Øystein Wiig, Simon Wilson, Jon Aars

United States Geological Survey: Staff Publications

This review critically evaluates the available mercury (Hg) data in Arctic marine biota and the Inuit population against toxicity threshold values. In particular marine top predators exhibit concentrations of mercury in their tissues and organs that are believed to exceed thresholds for biological effects. Species whose concentrations exceed threshold values include the polar bears (Ursus maritimus), beluga whale (Delphinapterus leucas), pilot whale (Globicephala melas), hooded seal (Cystophora cristata), a few seabird species, and landlocked Arctic char (Salvelinus alpinus). Toothed whales appear to be one of the most vulnerable groups, with …

Predictors Of Regional Establishment Success And Spread Of Introduced Non-Indigenous Vertebrates, Criag R. Allen, Kristine T. Nemec, Donald A. Wardwell, Justin D. Hoffman, Mathew L. Brust, Karie L. Decker, Daniel Fogell, Jennifer Hogue, Aaron Lotz, Thaddeus Miller, Marcy Pummill, Luis E. Ramirez-Yañez, Daniel R. Uden

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Aim To provide the first analysis of predictors of both establishment and spread, both within and across taxa, for all vertebrate taxa within a region.We used Florida, USA, as our study system because it has a well-documented history of introduction and invasion, and is a hotspot for biological invasions.

Location Florida, USA.

Methods We analysed non-indigenous species (NIS) data from peninsular Florida – which included both successful and unsuccessful introductions from all vertebrate classes – to determine the best predictors of both establishment and spread for fish (65 species), herpetofauna (63 species), birds (71 species) and mammals (25 species). We …

Parvalbumin In Fish Skin–Derived Gelatin: Is There A Risk For Fish Allergic Consumers?, S. J. Koppelman, J. A. Nordlee, P.-W. Lee, R. P. Happe, M. Hessing, R. Norland, T. Manning, R. Deschene, G. A. H. De Jong, S. L. Taylor

Department of Food Science and Technology: Faculty Publications

The major allergen parvalbumin was purified from cod muscle tissues, and polyclonal antibodies were raised toward it. The antibodies were tested for specificity, and an enzyme-linked immunosorbent assay (ELISA) was developed using these antibodies. The ELISA was applied to measure parvalbumin in cod skin, the starting material for fish gelatin made from deep sea, wild fish. The ELISA was sufficiently sensitive (LLOQ = 0.8 ng ml^–1 in extracts, corresponding to 0.02 μg of parvalbumin per g of tissue) and did not cross-react with common food constituents. Fish gelatin, wine, and beer, matrices for the potential use of this ELISA, …