Digital Commons Network^™

Selection Signatures In Four Lignin Genes From Switchgrass Populations Divergently Selected For In Vitro Dry Matter Digestibility, Shiyu Chen, Shawn M. Kaeppler, Kenneth P. Vogel, Michael D. Casler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass is undergoing development as a dedicated cellulosic bioenergy crop. Fermentation of lignocellulosic biomass to ethanol in a bioenergy system or to volatile fatty acids in a livestock production system is strongly and negatively influenced by lignification of cell walls. This study detects specific loci that exhibit selection signatures across switchgrass breeding populations that differ in in vitro dry matter digestibility (IVDMD), ethanol yield, and lignin concentration. Allele frequency changes in candidate genes were used to detect loci under selection. Out of the 183 polymorphisms identified in the four candidate genes, twenty-five loci in the intron regions and four loci …

Quantifying The Adaptive Cycle, David G. Angeler, Craig R. Allen, Ahjond S. Garmestani, Lance H. Gunderson, Olle Hjerne, Monika Winder

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The adaptive cycle was proposed as a conceptual model to portray patterns of change in complex systems. Despite the model having potential for elucidating change across systems, it has been used mainly as a metaphor, describing system dynamics qualitatively. We use a quantitative approach for testing premises (reorganisation, conservatism, adaptation) in the adaptive cycle, using Baltic Sea phytoplankton communities as an example of such complex system dynamics. Phytoplankton organizes in recurring spring and summer blooms, a well-established paradigm in planktology and succession theory, with characteristic temporal trajectories during blooms that may be consistent with adaptive cycle phases. We used long-term …

Genomewide Association Study Of Liver Abscess In Beef Cattle1,2, J. W. Keele, L. A. Kuehn, T. G. Mcdaneld, R. G. Tait, S. A. Jones, B. A. Keel, W. M. Snelling

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Fourteen percent of U.S. cattle slaughtered in 2011 had liver abscesses, resulting in reduced carcass weight, quality, and value. Liver abscesses can result from a common bacterial cause, Fusobacterium necrophorum, which inhabits rumen lesions caused by acidosis and subsequently escapes into the blood stream, is filtered by the liver, and causes abscesses in the liver. Our aim was to identify SNP associated with liver abscesses in beef cattle. We used lung samples as a DNA source because they have low economic value, they have abundant DNA, and we had unrestricted access to sample them. We collected 2,304 lung samples …

Surface Display Of Glycosylated Tyrosinase Related Protein-2 (Trp-2) Tumour Antigen On Lactococcus Lactis, Jeevanathan Kalyanasundram, Suet Lin Chia, Adelene Ai Lian Song, Abdul Rahim Raha, Howard A. Young, Khatijah Yusoff

Public Health Resources

Background: The exploitation of the surface display system of food and commensal lactic acid bacteria (LAB) for bacterial, viral, or protozoan antigen delivery has received strong interest recently. The Generally Regarded as Safe (GRAS) status of the Lactococcus lactis coupled with a non-recombinant strategy of in-trans surface display, provide a safe platform for therapeutic drug and vaccine development. However, production of therapeutic proteins fused with cell-wall anchoring motifs is predominantly limited to prokaryotic expression systems. This presents a major disadvantage in the surface display system particularly when glycosylation has been recently identified to significantly enhance epitope presentation. In this study, …

Contribution And Impact Of Iisers : A Scientrometric Assessment Of Publications During 2010-14, Visakhi P Dr, Ritu Gupta Miss, B. M. Gupta Dr

Library Philosophy and Practice (e-journal)

This paper analyzes 2542 research publications of the five Indian Institute of Science Education & Research (IISER) published during the last Five years (2010-14), as covered in SCOPUS International database. The study analyses characteristics of these publications such as the publication growth, research impact, national and international collaboration share, contribution and impact of authors and organizations, major areas of research, and preferred channels of research communications and characteristics of higher cited papers. The findings reveal that IISERs publications have witnessed an annual average growth rate of 34.92% and registered an average citation impact per paper of 9.90. About 33.39% and …

Hybridization Is Limited Between Two Lineages Of Freeze-Resistant Trichinella During Coinfection In A Mouse Model, Luke B.B. Hecht, Peter C. Thompson, Elizabeth S. Lavin, Dante S. Zarlenga, Benjamin M. Rosenthal

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Hybridization between two closely related but distinct genetic lineages may lead to homogenization of the two lineages with potentially novel phenotypes, or selective pressure to avoid hybridization if the two lineages are truly distinct. Trichinella nativa and Trichinella T6 are zoonotic nematode parasites which can be distinguished ge- netically despite occasional hybridization. Here, using an experimental murine model, we attempt to determine whether there are barriers to hybridization when sizeable numbers of each lineage are allowed to coinfect a host. Two mice were independently infected with equal numbers of T. nativa and T6. The offspring of these coinfections were genotyped …

Unique Pseudo-Expectations For C∗-Inclusions, David R. Pitts, Vrej Zarikian

Department of Mathematics: Faculty Publications

Given an inclusion D⊆C of unital C ∗ -algebras (with common unit), a unital completely positive linear map Φ of C into the injective envelope I(D) of D which extends the inclusion of D into I(D) is a pseudo-expectation. Pseudo-expectations are generalizations of conditional expectations, but with the advantage that they always exist. The set PsExp(C,D) of all pseudo-expectations is a convex set, and when D is Abelian, we prove a Krein–Milman type theorem showing that PsExp(C,D) can be recovered from its set of extreme points. In general, PsExp(C,D) is not a singleton. However, there are large and natural classes …

U.S. Drought Monitor, December 22, 2015, Richard R. Heim Jr.

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 22, 2015 (12/22/15) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

Acculturation And Alcohol Drinking Behavior Among Chinese International University Students In The Midwest, Shuangshuang Cai

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The purpose of this study was to explore the relationship between alcohol consumption and acculturation among Chinese international university students in the Midwestern part of the United States. A sample of 91 students from a university in the Midwest participated in the study. All were Chinese and included undergraduate and graduate students. Measures used included the General Ethnicity Questionnaire–Chinese Version (Abridged); the General Ethnicity Questionnaire–American Version (Abridged); the Alcohol Expectancy Questionnaire (AEQ-3); the Alcohol Use Questionnaire, consisting of two subscales, drinking frequency and drinking quantity; and a demographic form created by the author. Results indicated that older Chinese international students …

Architecture Of High-Affinity Unnatural-Base Dna Aptamers Toward Pharmaceutical Applications, Ken Ichiro Matsunaga, Michiko Kimoto, Charlotte Hanson, Michael Sanford, Howard A. Young, Ichiro Hirao

Public Health Resources

We present a remodeling method for high-affinity unnatural-base DNA aptamers to augment their thermal stability and nuclease resistance, for use as drug candidates targeting specific proteins. Introducing a unique mini-hairpin DNA provides robust stability to unnatural-base DNA aptamers generated by SELEX using genetic alphabet expansion, without reducing their high affinity. By this method, >80% of the remodeled DNA aptamer targeting interferon-γ (KD of 33 pM) survived in human serum at 37 °C after 3 days under our experimental conditions, and sustainably inhibited the biological activity of interferon-γ.

The Status Of Fishes In The Missouri River, Nebraska: Selected Ancient Fishes, Kirk D. Steffensen

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Several ancient fish species have inhabited the Missouri River and its tributaries for thousands of years prior to major mainstem modifications and fragmentation. However post-anthropogenic modifications, populations of these ancient fish species have been highly diminished. Therefore, the objective of this study was to use historic and current ichthyological records to determine the past and present status for Chestnut Lamprey Ichthyomyzon castaneus, Silver Lamprey Ichthyomyzon unicuspis, Bowfin Amia calva, American Eel Anguilla rostrata, and Burbot Lota lota. Currently, these species are rarely captured and perhaps extirpated from Nebraska waters.

Rnai-Mediated Control Of Aflatoxins In Peanut: Method To Analyze Mycotoxin Production And Transgene Expression In The Peanut/Aspergillus Pathosystem, Renee S. Arias, Phat M. Dang, Victor S. Sobolev

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Food and Agriculture Organization of the United Nations estimates that 25% of the food crops in the world are contaminated with aflatoxins. That represents 100 million tons of food being destroyed or diverted to non-human consumption each year. Aflatoxins are powerful carcinogens normally accumulated by the fungi Aspergillus flavus and A. parasiticus in cereals, nuts, root crops and other agricultural products. Silencing of five aflatoxin-synthesis genes by RNA interference (RNAi) in peanut plants was used to control aflatoxin accumulation following inoculation with A. flavus. Previously, no method existed to analyze the effectiveness of RNAi in individual peanut transgenic events, …

Evaluation Of Interallelic Waxy, HeteroWaxy, And Wild-Type Grain Sorghum Hybrids, M. K. Yerka, J. J. Toy, Deanna L. Funnell-Harris, Scott E. Sattler, J. F. Pedersen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Four near-isogenic Wheatland x Tx430 grain sorghum [Sorghum bicolor (L.) Moench] hybrids differing in allelic status at the Waxy locus were grown in yield trials to determine their potential to expand existing sources of low-amylose starch. The hypothesis tested was that agronomic performance and grain yield do not differ among hybrid genotypes. Hybrids were generated in a two-by-two factorial design using wx^b and wild-type (WT) Wheatland as female parents with wx^a and WT Tx430 as male parents. Yield trials were conducted at two Nebraska locations in 2009 and 2010. No differences were observed for field emergence, but …

A Desert In Disguise: The Resilience Of The Nebraska Sandhills, Jeff Hartman

School of Natural Resources: Dissertations, Theses, and Student Research

The Nebraska Sandhills are the largest sand dune system in the Western Hemisphere, and are unique because they remain relatively undisturbed from row crop agriculture. Research in the past two decades demonstrated that the Sandhills are dynamic on millennial timescales, switching between stabilized, vegetated states to non-vegetated, mobilized states. The Sandhills are currently stabilized, but understanding how ecological processes are altered as sand dunes transition from stabilized to mobilized states, provides insight into the thresholds, stability, and resilience of this grassland ecosystem. My research investigated the impacts of vegetation disturbances on ecological processes and the sand dune surface stability. For …

Phosphorus Release Potential Of Agricultural Soils Of The United States, Rebecca A. Young

School of Natural Resources: Dissertations, Theses, and Student Research

Phosphorus (P) is one of the leading causes of surface water quality decline in the United States, leading to algal blooms and hypoxia in lakes and streams. Decreasing conservation funds dictate that agencies such as the Natural Resources Conservation Service, maximizes its effectiveness and efficiency in implementing practices to address P management and runoff on agricultural lands. Additional information on P behavior in soil is needed to improve P management plans to reduce pollution risk at the watershed, farm, and field scales. This research focuses on the development of total soil P release models, to be included into assessment and …

Assessment Of Selected Antibiotic Resistances In Ungrazed Native Nebraska Prairie Soils, Lisa M. Durso, David A. Wedin, John E. Gilley, Daniel N. Miller, David B. Marx

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The inherent spatial heterogeneity and complexity of antibioticresistant bacteria and antibiotic resistance (AR) genes in manureaffected soils makes it difficult to sort out resistance that can be attributed to human antibiotic use from resistance that occurs naturally in the soil. This study characterizes native Nebraska prairie soils that have not been affected by human or food-animal waste products to provide data on background levels of resistance in southeastern Nebraskan soils. Soil samples were collected from 20 sites enumerated on tetracycline and cefotaxime media; screened for tetracycline-, sulfonamide-, b-lactamase–, and macrolide-resistance genes; and characterized for soil physical and chemical parameters. All …

Extension Agriculture And Natural Resources In The U.S. Midwest: A Review And Analysis Of Challenges And Future Opportunities, Mahdi M. Al-Kaisi, Roger Wesley Elmore, Gerald A. Miller, David Kwaw-Mensah

Department of Agronomy and Horticulture: Faculty Publications

This review addresses key changes in U.S. agricultural extension and future opportunities. Agricultural extension has been a part of the land-grant university (LGU) system for more than 100 years. The Morrill Act of 1862 established the LGU system by authorizing states access to federally controlled land and funding for public institutions offering educational opportunities focusing on agriculture and mechanical arts for farmers and the working class. Current surveys in Iowa reveal changing trends in extension: more than 90% of farmers identified private-sector crop advisers as their primary source for recommendations, whereas more than 80% of those advisors identified Iowa State …

Turning An Organic Semiconductor Into A Low-Resistance Material By Ion Implantation, Beatrice Fraboni, Alessandra Scidà, Piero Cosseddu, Yongqiang Wang, Michael Nastasi, Silvia Milita, Annalisa Bonfiglio

Nebraska Center for Energy Sciences Research: Publications

We report on the effects of low energy ion implantation on thin films of pentacene, carried out to investigate the efficacy of this process in the fabrication of organic electronic devices. Two different ions, Ne and N, have been implanted and compared, to assess the effects of different reactivity within the hydrocarbon matrix. Strong modification of the electrical conductivity, stable in time, is observed following ion implantation. This effect is significantly larger for N implants (up to six orders of magnitude), which are shown to introduce stable charged species within the hydrocarbon matrix, not only damage as is the case …

The Impact Of A Common Approach To Instruction Within A Nebraska Rural School District, Bret Allan Schroder

Department of Educational Administration: Dissertations, Theses, and Student Research

The purpose of this mixed methods study was to examine the instructional understanding and effectiveness of a district wide implementation of a Common Approach to Instruction. This research study provided a greater understanding of the affects that such an implementation had on certified staff regardless of grade level, experience, subject, or gender.

This explanatory, sequential, mixed methods study was conducted during the summer, spring, and fall of 2014-2015. The study initially gathered data using an online survey, based on Marzano’s 41 instructional elements, in a single class-B school district in Nebraska. All certified staff members within this school district were …

Affordable Care Act 2016 Changes, Marilyn Schlake

Cornhusker Economics

Since enacted in 2010, the Affordable Care Act (ACA) has in some capacity affected nearly all individuals and businesses throughout the United States. With its sweeping legislation, insurance companies, health providers, employers and individuals alike have changed how they operate within the health industry. These changes will continue as more ACA regulations go into effect in 2016 and 2017. Understanding how these changes affect you and your family and business is important to successfully navigate through the evolving ACA regulations.

Onciderini Thomson, 1860 (Coleoptera: Cerambycidae: Lamiinae) Types Of The Muséum National D’Histoire Naturelle (Mnhn), With A Brief History Of The Coleoptera Collection, Eugenio H. Nearns, Gérard L. Tavakilian

Insecta Mundi

The primary types of Onciderini Thomson, 1860 of the Muséum national d’Histoire naturelle (MNHN), Paris, are catalogued and illustrated. Data on the original combination, current name, gender, and type locality are verifi ed and presented. There are 139 primary types of Onciderini including 38 in Oncideres Lacordaire, 1830; 17 in Hypsioma Audinet-Serville, 1835; 10 in Hesycha Fairmaire and Germain, 1859; nine in Hypselomus Perty, 1832; and eight each in Eudesmus Audinet-Serville, 1835 and Trestonia Buquet, 1859. Of the 139 primary types, 71 were described by J. Thomson, 34 by H. W. Bates, 13 by the authors of this work, and …

U.S. Drought Monitor, December 15, 2015, Richard Tinker

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 15, 2015 (12/15/15) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

U.S. Vehicle Efficiency Standards, Derek Baylog

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

The United States is a driving force of the global economy and a major innovator in the technology sector. For decades the world has looked to The United States as a leader of great technological advancements with the strong economy to allow investors a chance make these technologies a reality. So why is it now that we lag behind other countries when it comes to making our technology energy efficient? Sure we’ve been steadily growing our production of renewable resources with wind and solar energy, bet we are still behind in the second largest source of pollution, transportation. According to …

When Addressing Climate Change, Don’T Forget Animal Agriculture, Aaron Matzke

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

As COP 21 closes in Paris, global animal agriculture is a topic that has eluded the international media. Animal agriculture is responsible for more global greenhouse gas emissions than transportation according to the U.N.’s Food and Agriculture Organization. With global population and prosperity rising, the global meat supply is expected to nearly double by 2050. This increase in production will only be achieved if significant steps are taken to promote sustainability through existing techniques globally, and to regulate air contamination in industrial feedlots in the United States. Alternative meat production methods, which have seen considerable investment recently, could supplement animal …

Dynamic Post Testing Review, Karla A. Lechtenberg, John D. Reid, Ronald K. Faller

Department of Civil and Environmental Engineering: Faculty Publications

The objective of this research study was to compile a database of existing dynamic post testing conducted globally and located in many different research reports for use internally during future Midwest Roadside Safety Facility (MwRSF) research studies.

Effects Of Repeated Quetiapine Treatment On Conditioned Avoidance Responding In Rats, Jun Gao, Min Feng, Natashia Swalve, Collin Davis, Nan Sui, Ming Li

Department of Psychology: Faculty Publications

The present study characterized the behavioral mechanisms of avoidance–disruptive effect of quetiapine in the conditioned avoidance response test under two behavioral testing (2 warning signals vs. 1 warning signal) and two drug administration conditions (subcutaneous vs. intravenous). In Experiments 1 and 2, well-trained adult male Sprague-Dawley rats were tested under the subcutaneous (s.c.) quetiapine treatment (5.0, 15.0, 25.0, 50.0 mg/kg) for 7 days in a novel procedure consisting of two conditioned stimuli (CS) (white noise serving as CS1 and pure tone as CS2). Only the highest dose (50.0 mg/kg) produced a persistent suppression of the avoidance response without impairing the …

Energy Subsidies And The Future Of Power, Christian Jewett

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

The modern political climate has grown rife with talk concerning the use of fossil fuels as an energy source and how this may impact our planet for generations to come. The Left and the Right seem to be at odds over whether or not climate change is actually occurring, or who is causing it. Additionally, the energy industry likes to tout their “low-cost” energy sources as a boon to the global economy as well as a benefit to the consumer at large. However, are coal, oil, and natural gas truly the “cheapest” sources of energy? Whether or not one believes …

Investigating The Factors Affecting The Level Of Job Satisfaction Among The Librarians At Central Library Of Islamic Azad University Of District 3, Mitra Ghiasi, Safie Tahmasebi Limoni

Library Philosophy and Practice (e-journal)

This research aims at investigating the level of job satisfaction in librarians working at libraries of Islamic Azad University in District 3. The research has analytical-survey method and the questionnaire and statistical sample consist of 40 librarians at target libraries. To conduct this research, a model is first designed for evaluating the job satisfaction in librarians in the form of 6 dimensions and 31 variables and their job satisfaction is designed based on the model and calculated by considering the scores of 31 variables. Based on the findings, the librarians' job satisfaction at Islamic Azad University is in a proper …

Making Walkable Communities, Katie Zuehlke

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

There is no doubt that global warming is happening. With all the rage about climate change and global warming people should be doing more about it than just reading about it in the news. We should be making efforts towards making changes on a local level. This in turn could grow to a national level with more and more people participating in the movement and actually make global changes, instead of just talking about it. When the scale of participants grows the scale of the changes made will grow as well and this will be the beginning of the changes …

Creativity Of Librarians In Public Libraries: Case Study Of Public Libraries Of Fars Province (Iran), Ali Biranvand, Faramarz Soheili, Ali Akbar Khasseh

Library Philosophy and Practice (e-journal)

Abstract

Purpose: This paper aims to investigate the contextual conditions of creativity based on Oldham Model among the librarians of public libraries of Fars province.

Design/methodology/approach: The research uses a survey method. The study population was librarians of public libraries in Fars province. The data collection instrument was questionnaire. For data analysis, univariate T test and covariance analysis and variance analysis were used to evaluate correlation between the contextual conditions of creativity and its components.

Findings: In public libraries of Fars province, the required conditions for creativity of librarians was above average. The personal differences of librarians …