Digital Commons Network^™

Fixing Coccidian Oocysts Is Not An Adequate Solution To The Problem Of Preserving Protozoan Type Material, Donald Duszynski, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Fresh (36 days old) sporulated oocysts of Eimeria nieschulzi were divided into 7 groups. Control oocysts were maintained at 23 C in 2% aqueous (w/v) K[sub 2]Cr[sub 2]O [sub 7]. The 6 experimental groups were mixed with either Bouin's solution, 10% aqueous (v/v) buffered formalin, Karnovsky's solution, glutaraldehyde, paraformaldehyde, or 70% aqueous (v/v) ethanol (EtOH). After 115 days, oocysts from all 7 groups were examined under oil immersion to determine the effect of fixation on their structural integrity. The parameters examined were lengths and widths of oocysts and sporocysts, percent sporulation (%S), and percent crenation (%C) of oocysts and sporocysts. …