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Oxygen Glucose Deprivation And Hyperthermia Induce Cellular Damage In Neural Precursor Cells And Immature Neurons, Luminita Luca
Oxygen Glucose Deprivation And Hyperthermia Induce Cellular Damage In Neural Precursor Cells And Immature Neurons, Luminita Luca
Open Access Dissertations
Hyperthermia damages both developing and adult brains, especially when it occurs after ischemia or stroke. Work presented in this dissertation used in vitro models of these stresses to investigate mechanisms underlying damage to immature neurons and neural precursors cultured from embryonic rat brain. Studies described in Chapter 2 investigated the effects of a brief, intense hyperthermic stress (30-45 min at 43ºC). This stress produced a selective depletion of nestin-immunoreactive neural precursor cells, and reduced proliferation, as evidenced by reduced BrdU incorporation into young Tuj1-immunoreactive neurons. The stress activated caspase 3, and produced multiple signs of nuclear damage as well as …
Light-Induced Relocalization Of The Photoreceptor G Protein Transducin Is Mediated By Binding Partner-Restricted Diffusion: New Insights Into G Protein Subunit Dissociation, Derek H. Rosenzweig
Light-Induced Relocalization Of The Photoreceptor G Protein Transducin Is Mediated By Binding Partner-Restricted Diffusion: New Insights Into G Protein Subunit Dissociation, Derek H. Rosenzweig
Open Access Dissertations
Phototransduction is a well characterized system for study of G protein coupled receptor (GPCR) signaling. The GPCR rhodopsin couples to the heterotrimeric G protein transducin. Light-stimulated activation of transducin in turn activates phosphodiesterase (PDE), leading to closure to cGMP-gated channels and inhibition of glutamate release. Rod and cone photoreceptors are highly polarized neurons consisting of the outer segment (OS) where phototransduction biochemistry occurs, the inner segment containing mitochondria and other organelles, the nuclear layer, an axon, and a glutamatergic synapse. Upon illumination, activated G protein transducin redistributes from the rod OS (where it is localized in the dark) to the …