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Metabolic Toxicity Studies Using High Throughput Bioreactor Assay And Liquid Chromatography Coupled With Mass Spectrometry (Lc-Ms/ Ms), Dandan Li
Doctoral Dissertations
Metabolic toxicity is one of main concerns in drug discovery and development. High efficient and low cost metabolic toxicity assessment is still a major challenge for pharmaceutical industry. This thesis focuses on the development of high throughput in vitro metabolic toxicity assay coupling with liquid chromatography tandem mass spectrometry for metabolic adducts generation, characterization and quantitation. The key technology was based on electrostatic fabrication of polyelectrolytes and biomolecules on magnetic beads to form bioreactors in 96 well plate format and acquired metabolites induced toxicity molecular information by LC-MS experiments.
Chapter one will address the goals and significance of the metabolic …