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Microengineering The Neural Tube, Christopher Demers
Microengineering The Neural Tube, Christopher Demers
Electronic Theses and Dissertations
Early embryonic development is a complex and highly regulated orchestra of instructive cues that collectively guide naïve stem cells towards progressively more specialized fates. In the neural tube, the precursor structure to the brain and spinal cord, these signals emanate from ‘organizing centers’ surrounding the neural tube. These organizing centers send out soluble cues or morphogens that diffuse tens to hundreds of microns to recipient cells residing in the neural tube. Re-creating this dynamic landscape of cues in vitro is impossible using standard cell culture tools and techniques. However, microfluidics is perfectly suited to fill this gap, allowing precise control …
Developmental Cis-Regulatory Analysis Of The Cyclin D Gene In The Sea Urchin Strongylocentrotus Purpuratus, Christopher Michael Mccarty
Developmental Cis-Regulatory Analysis Of The Cyclin D Gene In The Sea Urchin Strongylocentrotus Purpuratus, Christopher Michael Mccarty
Electronic Theses and Dissertations
Proper execution of animal development requires that it be integrated with cell division. In part, this is made possible due to cell cycle regulatory genes becoming dependent upon developmental signaling pathways that regulate their transcription. Cyclin D genes are important bridges linking the regulation of the cell cycle to development because these genes regulate the cell cycle, growth and differentiation in response to intercellular signaling. In this dissertation, a cis-regulatory analysis of a cyclin D gene, Sp-CycD, in the sea urchin, Strongylocentrotus purpuratus, is presented. While the promoters of vertebrate cyclin D genes have been analyzed, the cis-regulatory sequences across …