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Genetic Population Structure Of The Endemic Fourline Wrasse (Larabicus Quadrilineatus) Suggests Limited Larval Dispersal Distances In The Red Sea, Philadelphia University Sep 2010

Genetic Population Structure Of The Endemic Fourline Wrasse (Larabicus Quadrilineatus) Suggests Limited Larval Dispersal Distances In The Red Sea, Philadelphia University

Philadelphia University, Jordan

No abstract provided.


How Powerful Is The Ioc? – Let’S Talk About The Environment, Marc A. R. Zemel Aug 2010

How Powerful Is The Ioc? – Let’S Talk About The Environment, Marc A. R. Zemel

Marc A. R. Zemel

The International Olympic Committee (IOC) is in a unique position as the supreme administrator of an immensely popular international mega-event and a self-proclaimed champion of environmental issues and sustainable development. Every two years, cities from all over the world spend millions of dollars for the mere privilege of competing to host the Olympic Games, and those cities must play by the IOC’s rules. In addition, Article 2 of the Olympic Charter, the constitution-like instrument governing the IOC and the Olympic Movement, requires the IOC to ensure that the Olympics are held to promote sustainable development and show concern for the …


Euthanasia Methods In Field Settings For Wildlife Damage Management, Timothy J. Julien, Stephen M. Vantassel, Scott R. Groepper Mr., Scott E. Hygnstrom Jan 2010

Euthanasia Methods In Field Settings For Wildlife Damage Management, Timothy J. Julien, Stephen M. Vantassel, Scott R. Groepper Mr., Scott E. Hygnstrom

Scott R Groepper

No abstract provided.


Quantitativepcrmethods Forrna Anddnainmarine Sediments: Maximizing Yieldwhile Overcoming Inhibition, Karen Lloyd, Barbara J. Macgregor, Andreas Teske Jan 2010

Quantitativepcrmethods Forrna Anddnainmarine Sediments: Maximizing Yieldwhile Overcoming Inhibition, Karen Lloyd, Barbara J. Macgregor, Andreas Teske

Karen Lloyd

For accurate quantification of DNA and RNA from environmental samples, yield loss during nucleic acid purification has to be minimized. Quantitative PCR (qPCR) and reverse transcription (RT)-qPCR require a trade-off between maximizing yield and removing inhibitors. We compared DNA and RNA yield and suitability for quantitative SYBR Green PCR and RT-PCR using the UltraClean and PowerSoil extraction kits and a bead-beating protocol with phenol/chloroform extraction steps. Purification methods included silica-column-based procedures from the MoBio kits, RNeasy MinElute, WizardPlus miniprep columns, and an acrylamide gel extraction. DNA and RNA purification with WizardPlus and RNeasy, respectively, led to significant losses of nucleic …