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Occidental College

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Distribution And Dietary Regulation Of An Associated Facultative Rhizobiales-Related Bacterium In The Omnivorous Giant Tropical Ant, Paraponera Clavata, Hannah Larson, Shana Goffredi, Erica Parra, Orlando Vargas, Adrian Pinto-Tomas, Terrence Mcglynn May 2014

Distribution And Dietary Regulation Of An Associated Facultative Rhizobiales-Related Bacterium In The Omnivorous Giant Tropical Ant, Paraponera Clavata, Hannah Larson, Shana Goffredi, Erica Parra, Orlando Vargas, Adrian Pinto-Tomas, Terrence Mcglynn

Shana Goffredi

We document a facultative Bartonella-like Rhizobiales bacterium in the giant tropical ant, Paraponera clavata. In a lowland tropical rainforest in Costa Rica, 59 colonies were assayed for the prevalence of the Bartonella-like bacterium (BLB), 14 of which were positive. We addressed three questions: First, how does the prevalence of BLB within colonies vary with environmental conditions? Second, how does diet affect the prevalence of BLB in P. clavata? Third, how does the distribution of BLB among colonies reflect ambient differences in food resources and foraging habits? A variety of environmental variables that may be predictive of the presence of BLB …


Ontogenetic Variation In Epibiont Community Structure In The Deep-Sea Yeti Crab, Kiwa Hirsuta: Convergence Among Crustaceans, Shana Goffredi, Ann Gregory, William Jones, Norma Morella, Reid Sakamoto Mar 2014

Ontogenetic Variation In Epibiont Community Structure In The Deep-Sea Yeti Crab, Kiwa Hirsuta: Convergence Among Crustaceans, Shana Goffredi, Ann Gregory, William Jones, Norma Morella, Reid Sakamoto

Shana Goffredi

Recent investigations have demonstrated that unusually 'hairy' yeti crabs within the family Kiwaidae associate with two predominant filamentous bacterial families, the Epsilon and Gammaproteobacteria. These analyses, however, were based on samples collected from a single body region, the setae of pereopods. To more thoroughly investigate the microbiome associated with Kiwa puravida, a yeti crab species from Costa Rica, we utilized barcoded 16S rRNA amplicon pyrosequencing, as well as microscopy and terminal restriction fragment length polymorphism analysis. Results indicate that, indeed, the bacterial community on the pereopods is far less diverse than on the rest of the body (Shannon indices ranged …