Digital Commons Network^™

Comparison Of The Effects Of Catchment Land Use/Land Cover On Freshwater Macroinvertebrate Communities And Water Quality In Three Rivers Of The Dingle Peninsula, Brooke Elizabeth Chesler

Theses

The Dingle Peninsula, a dynamic coastal ecosystem in the southwest of Ireland, provides an appropriate location to study the delicate balance that exists between human activities i.e., tourism, agriculture, and forestry, and their resulting impacts on the environment. Thus, the adjacent Feohanagh, Milltown, and Owenmore catchments were analysed via three-minute kick-samples divided proportionally by substrata for macroinvertebrate community composition, in-situ physico-chemical measurements for water quality parameters, and CORINE classification of land use and land cover (LULC). The overall goal was to identify the effect of LULC on water quality using macroinvertebrates as bioindicators. The presence of sensitive or tolerant macroinvertebrates …

‘A Healthy Mtu’ A Mixed Methods Baseline Programme Of Research To Empirically Inform A Campus Health Promotion Initiative Within An Irish Higher Education Setting, Andrea Bickerdike

Theses

Higher Education Institutions (HEIs) have been called to lead global health promotion actions, yet there remains a dearth of empirically informed ‘Healthy University’ (HU) initiatives. This thesis constituted a baseline mixed methods needs assessment to inform a settings-based health promotion (HP) initiative (‘A Healthy MTU’) within a multi-campus ‘case’ HEI in Ireland (Cork campuses of MTU, formerly CIT). Specific aims were to (i) investigate student and staff health metrics, and (ii) identify the cultural and environmental determinants of health and wellbeing within the case HEI setting. Using a phased, mixed methodology (QUAN-QUAL), five sequential empirical studies were undertaken. Quantitative data …

The Use Of Membrane Filtration To Increase Native Whey Protein In Infant Formula, And Effects On Bioaccessibility And Bioactivity, Yihong Chen

Theses

Breastfeeding is recognised as the gold standard for feeding newborns and infants. However, infants who cannot be breast-fed or for whom breast milk is not available, can be fed with bovine infant milk formula (IMF) as a substitute. Researchers and producers of IMF strive to continually improve or “humanise” the composition and functionality of IMF. During production of IMF it must undergo several heat treatment steps, e.g. heat-sterilisation or spray-drying, in order to provide a safe product to the infant. These heat treatments denature the globular whey proteins in IMF. In contrast, breast milk is by its nature unheated, so …

The Impact Of Weighted Football Training On The Skill Acquisition Processes Of Place-Kicking In Sport, Sam Jermyn

Theses

Weighted implement training (WIT) involves performance of sport-specific motor skills with heavier-than-normal sporting implements. Typically, full range of motion (ROM) and similar kinematic characteristics of the respective motor skill are maintained concurrent to minimal changes in the force-velocity movement profile. While the majority of WIT research has been conducted in baseball, track and field, and cricket, there is a paucity of research conducted in the football codes, particularly Gaelic football. Existing observations of increased standard implement velocity and distance following the use of weighted implements infers potential benefits for Gaelic football performance, such as increasing kick-out ball velocity (BV) and …

A Different Perspective: An Exploration Towards The Emancipation Of Practice Within Social Care, Jonathan Condon

Theses

In Ireland today, it is recognized that the Reflexive Practitioner is the dominant paradigm within the district of Social Care and the approach of professionals is obtained through the traditional ways; whilst Social Pedagogy is still a relatively new import with only a couple of organizations operating across the country. Both disciplines work with the same general clientele and have similar goals for the clients with whom they work (Lalor and Share, 2013). The professional approach of both disciplines was unfastened and discovered throughout this study. Social care professionals focus on the protection of those in their care. Along with …

Nutrient Intakes, Compliance With Recommendations And Key Sources In Women Of Child-Bearing Age (18-50 Y) In Ireland, Abigail O' Mahony

Theses

Women’s pre-conceptional health (including nutritional status) is important for both the health of the individual themselves and also for the lifelong health of any occurring offspring. However, it has been estimated that up to 50% of pregnancies are unplanned, thus optimal nutritional status is important for all women of child-bearing age (WCBA) not just those with pregnancy intentions. Despite the accumulation of evidence of the importance of nutritional status at this life-stage, nutrient recommendations for WCBA for the most part don’t differ from recommendations for other population groups. The notable exception to this is that all WCBA are recommended to …

An Evaluation Of The Psychosocial Benefits For Children Experiencing Homelessness Arising From The Introduction Of An Integrated Community Care Project Across The City Of Cork, Traolach O' Callaghan

Theses

The purpose of the research is to evaluate an intervention within a new model of family support being delivered in Cork City, on addressing child and family homelessness. This new model is being delivered for the first time in Ireland during ‘Youth Club’ and strives to act as an intervention to assist children experiencing homelessness. The model was first developed by Tusla Springboard Child and Family service and Good Shepherd Services Cork in 2016 in response to the alarming rise in child and family homelessness in Cork city, and the resulting impact of same. The research investigates the model and …

Identification And Creation Of Novel Bacteriocins With Potential Food And Clinical Applications, David Lynch

Theses

Bacteriocins represent a heterogeneous group of small, ribosomal-synthesized, potent antibacterial peptides produced by bacteria, capable of inhibiting bacteria both closely related or indeed those from other genera than the producer. In addition to their role as food preservatives, bacteriocins have potent activity against medically significant pathogens and are considered attractive alternatives to antibiotics, due to their inherent heat stability, potency at nanomolar scale, resistance to proteases and low levels of acquired resistance in commercial applications. The aim of this thesis was to identify, purify and bioengineer novel bacteriocins from Staphylococcus and Lactic Acid Bacteria (LAB), with a view to expanding …

Investigating Optimal Lipid:Protein Profiles For Infant Milk Formula, Valentina Ivanova

Theses

From the early twentieth century onward, much research has focused on improving infant milk formula (IMF). During the development of IMF, breast milk is commonly considered as the reference and IMF manufacturers are thriving to mimic the composition and/or to match the functionality of breast milk as closely as possible. A variety of bioactive ingredients, found in human breast milk, have been isolated and investigated for their potential functionality and utilised in the production of IMF. This study evaluated the current knowledge on compositional differences between human breast milk and IMF, and recent advances and developments in IMF production. Following …

Genetic Selection For Improved Production Efficiency In The Mature Dairy Herd, Maeve Williams

Theses

Improving dairy cow production efficiency is highly desirable because of its well-documented impact on profitability and sustainability. Long-term gains in dairy cow production efficiency have already been delivered through genetic selection. Nonetheless, achieving further improvements in production efficiency could cement the economic and environmental efficiency of the dairy industry, globally. To date, several components contributing to production efficiency have been overlooked, with many studies focusing on dairy cow production efficiency over short periods rather than lifetime efficiency. Therefore, the objectives of this thesis were to quantify the genetic variance components of complementary dairy cow efficiency metrics; including dry matter intake, …

Performance Analysis Of Zero Trust In Cloud Native Systems, Simone Rodigari

Theses

Critical applications demand strong security implementations, low latency and high availability at constant rates, however, the performance of a software system is affected by the implementation of security. This research measures the performance overhead and possible mitigation in cloud native systems secured with a service mesh, which allows enabling security policies for the authentication, authorization and encryption of traffic within distributed systems. The side-car proxy is a core component of this architecture, acting as a policy enforcement point and intercepting networking communication from/to applications part of the mesh, consequently affecting the performance of applications hosted in the cloud. Physical resources …

Isolation And Characterisation Of Soil-Derived Bacteria With Antimicrobial Activity Against Antibiotic Resistant Pathogens, Ellen Twomey

Theses

Bacteriocins are a diverse range of ribosomally synthesized bioactive peptides, recognised predominantly for their potent antimicrobial activity against other strains closely related to the producer. The focus of this thesis was to explore this activity and examine the potential of bacteriocins to inhibit the growth of clinically relevant pathogens. Although evidence exists in the literature to suggest that bacteriocins could be applied in a similar fashion to conventional antibiotics, to date they are only utilised as preservative in food to prevent the growth of foodborne illness causing organisms. Isolating and screening bacterial strains in order to identify bacteriocin producers has …

An Evaluation Of A Health, Wellbeing And Lifestyle Promotion Intervention Among A Cohort Of Year 1 Male Gaelic Games Student Athletes In An Irish University Setting, Anthony Casey

Theses

Background: The current research investigated the health, wellbeing and lifestyle behaviours of a cohort of 1st year male Gaelic Games student athletes (Study 1), and subsequently evaluated the impact of a bespoke intervention on these health, wellbeing and lifestyle behaviours in the same cohort (Study 2).

Methods: Participants were 1st year male Gaelic Games student athletes (mean age 18.6 ± 0.6 years, n=166) in a university in southern Ireland. Data were collected using a mixed-methods approach during the 2018/19 and the 2019/20 academic years. Data collection consisted of an online questionnaire (Bickerdike et al., 2018), which assessed health, wellbeing …

Exploring The Metabolic Capabilities And Biotransformation Potential Of Environmental Lactococcus Strains, Desirée Román Naranjo

Theses

The aim of this thesis is to characterize environmental Lactococcus strains to identify different and diverse metabolic capabilities that could be applied to microbial biotransformation, defined as the conversion of a cheap/waste substrate into a value-added product. Due to the increased amounts of food processing waste generated worldwide and the impact of climate change, new sustainable approaches are necessary to contribute to waste valorization and the circular bioeconomy. We ask to what extent and in what ways environment isolated Lactococcus strains could contribute to food biotransformation by metabolizing a different range of substrates. We do so to further understand and …

Why Post- Leaving Certificate Students In Ireland From Disadvantaged Backgrounds Are Less Likely To Proceed To Third-Level Education, Edward Hayes

Theses

Educational disadvantage continues to be a cause for concern, and addressing it remains at the forefront of education policy in Ireland (Houses of Oireachtas, 2019; Weir et al., 2017). This research extended across the academic years 2019-2020 and 2020-2021 and during the period of the global COVID-19 pandemic, which imposed public health measures, and enforced an emergency online digital learning environment. A socio-economic profile of Ireland’s student body registered an attainment gap between rich and poor, finding that young people from backgrounds of disadvantage continue to be underrepresented at third-level when compared to their middle-class counterparts (HEA, 2020). The primary …

An Investigation Of ‘Restrictive Practices’ Within Services For Adults With An Intellectual Disability: Perspectives Of Professionals And Those Involved With Their Care., Rebecca Donovan

Theses

Restrictive practices “are an infringement of a person’s fundamental rights to personal liberty and bodily integrity” (Health Information and Quality Authority, 2019, p.1). The most recent study in Ireland, found 23% of the 627 inspection reports by HIQA, human-right were violated by the use of restrictive practices (Murphy and Bantry- White, 2021). It is recognised that the core function of restrictive practices is to stop, or prevent, an individual from doing something they wish to do, in such a way that manages challenging behaviour outbursts, and provides safety for both staff and service users (Nankervis and Chan, 2021). The uncertain …

Tailoring The Rehabilitative Environment For People With Sensory Overload, Jennifer Cotter

Theses

Sensory overload is a complex sensory processing disorder which can develop post Acquired Brain Injury (ABI). The condition can be extremely debilitating and people who develop it are further hampered by lack of informational resources, sensory overload is currently under researched in the Acquired Brain Injury rehabilitation sector. This qualitative study documents participants first hand experiences of sensory overload post ABI and includes insights and observations from professionals working in the Acquired Brain Injury rehabilitation sector in Ireland. In this study, sensory overload is the focal point and a new rehabilitation tool in the form of a sensory menu is …

The Irish Early Childhood Care And Education (Ecce) Scheme Is Free And Universal, But At Whose Expense?, Johanna Forde

Theses

This research addresses the historical and current influences on quality practice and provision by reviewing national and international literature debating quality in the Irish Early Childhood Care and Education (ECCE) scheme (DCEDIY, 2021a). To understand quality components the regulations and policy frameworks that inform and guide the ECCE scheme (DCEDIY, 2021a), including ECCE practice and the implications for provision standards, are explored. Four research questions ask: (1) What constitutes quality provision for children attending the ECCE scheme (DCEDIY, 2021a)? (2) What has informed ECCE regulation and policy in Ireland? (3) What are the identified challenges associated with the ECCE scheme …

Determining Suitable Green Biorefinery Locations In Ireland For Irish Agriculture Using Co-Design, Economic And Geographical Information Systems, Alice Hand

Theses

The aim of this thesis is to investigate potential locations for a suitable green biorefinery model in Ireland, which would offer an opportunity for sustainable diversification to grassland farmers. A mixed method approach was used to collect data to support the design and analyse a green biorefinery model. A three phased methodology was applied representing the Co-design, Economic and the Geographical Information Systems analysis phase. Key stakeholders identified in the co-design phase included farmers, cooperatives, market partners as having a direct impact on a biorefinery. This provided insight to the farmers preferred model. Using these findings, an economic assessment was …

Miniaturized Iii-V/ Si Hybrid Laser With An Integrated Modulator, Praveen Kumar Singaravelu

Theses

Light interaction with microscopic and nanoscopic structures enable manipulation of its characteristics which can be used to detect objects in 3D sensing, propel satellites to space using photonic propulsion and transmit data through optical communication. For optical communication, the basic components are lasers, modulators and photodetectors. The development of CMOS microfabrication foundries helps to manufacture silicon-based photonic devices with high yield that is directly co-integrated with electronics in a single chip. However, the lack of emission of photons efficiently in silicon propelled the necessity of hybrid photonic devices that inherits the combined advantage of different materials i.e. functionality and volume. …

A New Method For Cluster Life Cycle Stage Identification: Mapping The Evolution Of The Biomedical Competitiveness Cluster Of Wallonia., Conor Michael Harte

Theses

National governments and policy makers alike need data and expertise as they seek to develop accurate and relevant cluster policies and initiatives. It is argued that clusters also have life cycles similar to the way industries, products, and technologies have a life cycle, including a variety of stages (Ingstrup and Damgaard, 2013; Jia et al., 2015; Fornahl and Hassink, 2017; Ferrari et al., 2020). This research addresses three objectives: 1. Analyse the longitudinal development of a cluster to record, measure and analyse its evolution over a three-year period; 2. Create a new methodology to support cluster life cycle stage identification; …

Advancing Industrial Participation In Demand Response Within National Electricity Grids Through Systematic Asset Selection, Risk Assessment And Modelling Approaches, Alexander Brem

Theses

Increasing the flexible capacity on national electricity grids will be key to maintain reliable control as the levels of renewables continue to increase annually. The industrial sector has been identified as being capable of contributing and potentially offering flexible capacities, following appropriate investigation. The aim of this thesis is to develop the knowledge, advance the engagement and encourage additional participation in national demand response programmes from the industrial sector to provide this flexibility. The potential within this sector, facilitated by the smart grid and demand response concepts is outlined, specifically detailing their benefits, driving factors and potential barriers as part …

Development Of Phage Therapy Strategies Against Gram-Positive Bacterial Pathogens, Sara Arroyo Moreno

Theses

Bacterial resistance to conventional antibiotics has become an urgent healthcare issue worldwide. In this light, a renewed interest has emerged for therapy with bacteriophages (phages), which are viruses that can specifically infect bacteria. These viruses encode different proteins that degrade specific bonds within the bacterial cell wall, both to inject their genomic material into the bacterial cells and to release the viral progeny: virion-associated hydrolases and endolysins, respectively. Elimination of Gram-positive pathogens with recombinant versions of phage enzymes represents a promising alternative to classical antibiotics. In this thesis, different phage therapy strategies involving either whole phages or the use of …

A Study On Plant-Derived Small Proteins And Peptides With Antiyeast Activity And Preliminary Investigations In Food Materials, Giulia Mignone

Theses

The discovery of novel antimicrobial molecules would benefit considerably different biotechnological fields, including medicine, agriculture and the food industry, on which this thesis work focuses. Specifically, here are presented fundamental studies on plant-derived antimicrobial proteins for the reduction of food spoilage caused by yeast microorganisms. Small proteins and peptides from the plant innate immune system possess several characteristics which could make them ideal candidates for a novel and natural antispoilage agent. The first Chapter reviews proteins families associated with the plant innate immune system that exhibit antiyeast activity and low molecular weight (<30 kDa), focusing on their structure and antiyeast mode of action. Chapter 2 investigates two peptides, Pn-AMP1 and Pn-AMP2 belonging to the hevein-type peptides family; peptides were synthetically generated using their native amino acid sequence. Synthetic Pn-AMP1 showed fast action activity against common food spoilage yeasts, it resulted potentially safe for consumption, and it was successfully incorporated in UHT milk and Fanta Orange, where it contained the growth of Kluyveromyces lactis and Zygosaccharomyces bailii, respectively. A different family of antimicrobial plant proteins (2S albumins) was studied in Chapters 3, 4, 5 and 6. Chapter 3 describes the selective extraction of an antiyeast protein (labelled WMS1 in this thesis work) of ~14 kDa from white mustard (Brassica hirta) seeds. The protein was purified in a relatively high yield, using a simple protocol that included only one chromatographic method step. In Chapter 4, bioinformatics tools and wet-lab techniques are used to study the structure and classify the antiyeast protein. The WMS1 antiyeast protein was identified as an isoform of the Napin protein Allergen Sin a 1 belonging to the 2S albumins family, characterised by high structural stability which donates to the protein resistance heat treatment and proteolytic degradation. Chapter 5 describes the antimicrobial spectrum of WMS1 , its antiyeast mechanism of action and its validity as preservative agent. Although WMS1 resulted potentially safe for consumption and inhibited the growth of Z. bailii in several beverage systems, its biotechnological potential is limited by its allergic properties. In Chapter 6, the protein WMS1 was cloned in a pET28a-MBP vector and expressed in an E. coli BL21 system. Interestedly, recombinant WMS1 and native WMS1 showed similar activity against the yeast Z. bailii. Thesis abstract 2 Chapter 7 includes a protocol for the rational design of ultra-short antimicrobial peptides. Since ultra-short antimicrobial sequences can be found within the sequence of any larger protein, the protocol was applied using WMS1 primary sequence, and seven ultra-short SinA-AMPs were chemically generated. Among the various SinA-AMPs, the peptide labelled SinA-pepIII had in vitro activity against several common yeast spoilage strains via membrane permeabilisation and it could be employed as a food preservative as it was pH- and heat- stable, potentially safe in terms of human consumption and it prevented the growth of Z. bailii in cranberry juice. The results obtained in this work offer new perspectives in the research for novel and clean-label food preservatives to fight the food waste phenomenon.

An Empirical Investigation Into Current And Future Overheating Frequency In Nzeb And Passivhaus Dwellings In Ireland, Evan Finegan

Theses

The European Performance of Buildings Directive (EPBD) 2011, ensures that all new Irish dwellings designed after 01st November 2019 are nearly zero energy (nZEB) dwellings. The Passive House (PH) standard has been the most commonly applied energy standard across the globe in recent decades, while overheating is identified as a growing issue in nZEB and PH dwellings. The EPBD requires all dwellings to be designed to reduce energy consumption, while considering general indoor climate conditions to avoid the negative effects of overheating. Overheating frequency simulations are not mandatory for dwellings under Irish legislation. Anticipated climate change trends will result in …

Mos Compatible Deposited Materials For Optical Interconnects, Simone Iadanza

Theses

The recent years' exponential growth of data transmission in datacom and telecom technology (such as in Wavelength Division Multiplexing - WDM) has highlighted the energy consumption challenges of electronics, shifting research and industry interest towards optical interconnects. Novel and more efficient integrated photonics is being given the objective to face the ever-increasing demand for speed and bandwidth. This has initiated, in both industrial and academic environments, a quest for low-power, compact and cost-effective optical interconnects that can be integrated on electronic boards of Datacentres and transceivers all over the world. In this thesis, different types of WDM compatible Hybrid External …

Robust Control Strategies For Optimal Operation Of Distributed Generation In Smart Microgrids, Chi-Thang Phan-Tan

Theses

The high penetration of PV systems and fast communications networks increase the potential for PV inverters to support the stability and performance of smart grids and microgrids. PV inverters in the distribution network can work cooperatively and follow centralized and decentralized control commands to optimize energy production while meeting grid code requirements. However, there are older autonomous inverters that have already been installed and will operate in the same network as smart controllable ones. This research proposes a decentralized optimal control (DOC) that performs multi-objective optimization for a group of PV inverters in a network of existing residential loads and …

Genetic Selection For Improved Efficiency And Profitability In Beef Cattle, David Kelly

Theses

Despite the importance of feed intake and efficiency in beef production, there are still gaps in our knowledge on optimal production efficiency traits and breeding strategies for improved feed, production and economic efficiency. The objectives of this thesis were to redefine production efficiency in growing cattle by exploiting carcass information and to validate current beef breeding goals for improving efficiency, and to investigate the relationships of feeding behaviour with performance, feed efficiency, and carcass merit. Feed intake and liveweight data were available on up to 6,088 cattle, of which 3,146 had carcass data, 4,672 had ultrasound data and 1,548 had …

Model-Based Cognitive Communications For Low-Power Wireless Networks, Indika Sanjeewa Abeywickrama Dhanapala

Theses

Our world is increasingly being instrumented with low-power wireless networks that deliver data for diverse application domains such as energy management, health and well-being, security, and other Internet of Things services. As these wireless networks operate in the unlicensed radio spectrum while co-existing with various other communication networks, their communication reliability and energy efficiency are affected by the radio environment. Because of this vulnerability to radio interference, low-power wireless networks may be unable to deliver their application requirements and be inefficiently consuming energy, functioning as a low-dependability network. The situation is exacerbated in indoor environments such as office and residential …

Elucidation Of Novel And Established Campylobacter Species With Clinical And Agricultural Significance Through Phenotypic, Genotypic, And Taxonomic Investigation, Caoimhe Lynch

Theses

Campylobacter spp. are well-established human, veterinary and economic pathogens, with a broad host range spanning from terrestrial and marine mammalian, avian and reptilian hosts. The scope of the study includes novel and notorious species within the genus, with reference to zoonotic agents Campylobacter jejuni and Campylobacter coli - the leading cause of human bacterial gastroenteritis in the EU and Ireland, Campylobacter fetus that represents the most common campylobacter causing bacteraemia and two novel species isolated from pigs not described in the literature previously. C. jejuni and C. coli isolates (n = 350) recovered from broiler caecal and neck skin samples, …