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The Use Of Tlr Ligands And Phytochemicals To Better Understand Gut Immunity In Zebrafish And Channel Catfish, Ann Elizabeth Peterman
The Use Of Tlr Ligands And Phytochemicals To Better Understand Gut Immunity In Zebrafish And Channel Catfish, Ann Elizabeth Peterman
Theses and Dissertations
Toll-like receptor (TLR) ligands and phytochemical feed additives (PFAs) were evaluated in this study to determine the effects of immune stimulation on gut immunity in the zebrafish, Danio rerio, and the channel catfish, Ictalurus punctatus. Rag1-/- (MT) zebrafish were used to study how the TLR ligands β-glucan and resiquimod (R848) affect the innate immune system in the gut of MT zebrafish. Enhanced expression of marker genes (NITR9, NCCRP-1 and MPEG-1) indicated stimulation of Natural Killer (NK) cells, non-specific cytotoxic cells (NCCs) and macrophages. After challenge with Edwardsiella ictaluri, MT zebrafish stimulated with β-glucan demonstrated higher survival and the presence of …
Innate And Adaptive Immune Responses Of Channel Catfish To Edwardsiella Ictaluri Wild Type And Live Attenuated Vaccine Candidates, Ozgur Erdogan
Innate And Adaptive Immune Responses Of Channel Catfish To Edwardsiella Ictaluri Wild Type And Live Attenuated Vaccine Candidates, Ozgur Erdogan
Theses and Dissertations
Edwardsiella ictaluri causes enteric septicemia of catfish (ESC), a devastating disease in the channel catfish industry. Our research group has developed several E. ictaluri live attenuated vaccine (LAV) candidates (EiΔevpB, EiΔevpBΔfur, EiΔevpBΔhfq, EiΔevpBΔfurΔhfq), which were able to stimulate an immune response in vaccinated channel catfish and reduce ESC. However, innate, and adaptive immune responses in the lymphoid tissues of channel catfish to these LAVs are not known well. The overall goal of the project is to determine the role of adaptive and innate immune responses in catfish after vaccination with LAVs. Analysis of innate and adaptive immune-related gene expressions showed …
Improved Monitoring And Decision-Making To Manage Atypical Aeromonas Hydrophila In Catfish Aquaculture Ponds, Bradley Richardson
Improved Monitoring And Decision-Making To Manage Atypical Aeromonas Hydrophila In Catfish Aquaculture Ponds, Bradley Richardson
Theses and Dissertations
Commercial catfish production is an inveterate industry within the southeastern United States. Bacterial disease is a significant detriment to global aquaculture, including the United States catfish industry. Among them, an atypical strain of the bacterium Aeromonas hydrophila has plagued the industry since the late 2000s. Atypical A. hydrophila (aAh) outbreaks are largely acute, resulting in catastrophic losses. The disease ecology, prevalence, and genetic distribution are poorly understood. Atypical Aeromonas hydrophila displays a rapid onset with few warning signs of the impending disease, making it difficult for early detection. At present there are two recognized haplotypes of aAh. This project aimed …
Intraspecific Variability Of Edwardsiella Piscicida And Cross-Protective Efficacy Of A Live-Attenuated Edwardsiella Ictaluri Vaccine In Channel And Channel × Blue Hybrid Catfi, Adrian Lopez Porras
Intraspecific Variability Of Edwardsiella Piscicida And Cross-Protective Efficacy Of A Live-Attenuated Edwardsiella Ictaluri Vaccine In Channel And Channel × Blue Hybrid Catfi, Adrian Lopez Porras
Theses and Dissertations
Incidence and prevalence of Edwardsiella piscicida has increased in Mississippi farm-raised catfish in recent years. Edwardsiella piscicida affects mostly market-sized catfish during the final stages of the production cycle resulting in significant economic losses. The objectives of this study were to determine the genetic variability of E. piscicida, assess virulence in channel and hybrid catfish, and evaluate the capacity of a live-attenuated E. ictaluri vaccine to protect channel and hybrid catfish against heterologous E. piscicida isolates. This work identified five discrete E. piscicida lineages, along with group specific associations of several virulence related genes. In general, E. piscicida was shown …