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Articles 1 - 9 of 9
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Genetic Locus Required For Antigenic Maturation Of Rhizobium Etli Ce3 Lipopolysaccharide, Dominik M. Duelli, Andrea Tobin, Jodie M. Box, V.S. Kumar Kolli, Russell W. Carlson, K. Dale Noel
Genetic Locus Required For Antigenic Maturation Of Rhizobium Etli Ce3 Lipopolysaccharide, Dominik M. Duelli, Andrea Tobin, Jodie M. Box, V.S. Kumar Kolli, Russell W. Carlson, K. Dale Noel
Biological Sciences Faculty Research and Publications
Rhizobium etli modifies lipopolysaccharide (LPS) structure in response to environmental signals, such as low pH and anthocyanins. These LPS modifications result in the loss of reactivity with certain monoclonal antibodies. The same antibodies fail to recognize previously isolated R. etli mutant strain CE367, even in the absence of such environmental cues. Chemical analysis of the LPS in strain CE367 demonstrated that it lacked the terminal sugar of the wild-type O antigen, 2,3,4-tri-O-methylfucose. A 3-kb stretch of DNA, designated as lpe3, restored wild-type antigenicity when transferred into CE367. From the sequence of this DNA, five open reading frames …
Functional And Structural Adaptations Of Skeletal Muscle To Microgravity, Robert Fitts, Danny R. Riley, Jeffrey J. Widrick
Functional And Structural Adaptations Of Skeletal Muscle To Microgravity, Robert Fitts, Danny R. Riley, Jeffrey J. Widrick
Biological Sciences Faculty Research and Publications
Our purpose is to summarize the major effects of space travel on skeletal muscle with particular emphasis on factors that alter function. The primary deleterious changes are muscle atrophy and the associated decline in peak force and power. Studies on both rats and humans demonstrate a rapid loss of cell mass with microgravity. In rats, a reduction in muscle mass of up to 37% was observed within 1 week. For both species, the antigravity soleus muscle showed greater atrophy than the fast-twitch gastrocnemius. However, in the rat, the slow type I fibers atrophied more than the fast type II fibers, …
Characterization Of Transepithelial Potential Oscillations In The Drosophila Malpighian Tubule, Edward M. Blumenthal
Characterization Of Transepithelial Potential Oscillations In The Drosophila Malpighian Tubule, Edward M. Blumenthal
Biological Sciences Faculty Research and Publications
The Malpighian tubule of Drosophila melanogaster is a useful model system for studying the regulation of epithelial ion transport. In acutely isolated tubules, the transepithelial potential (TEP) undergoes large oscillations in amplitude with a period of approximately 30s. The TEP oscillations are diminished by reductions in the peritubular chloride concentration in a manner consistent with their being caused by fluctuations in chloride conductance. The oscillations are eliminated by pretreating tubules with the calcium chelator BAPTA-AM, although removal of peritubular calcium has no effect, suggesting that the oscillations are a result of either the release of calcium from intracellular stores or …
Rna Levels And Activity Of Flowering Locus C Are Modified In Mixed Genetic Backgrounds Of Arabidopsis Thaliana, Michael Schläppi
Rna Levels And Activity Of Flowering Locus C Are Modified In Mixed Genetic Backgrounds Of Arabidopsis Thaliana, Michael Schläppi
Biological Sciences Faculty Research and Publications
Flowering time and FLOWERING LOCUS C (FLC) RNA levels were analyzed in different accessions of Arabidopsis thaliana and in mixed genetic backgrounds resulting from crosses between accessions. Dominant alleles of FRIGIDA (FRI) promote accumulation of FLC RNA, which in turn promotes late flowering. Although the coding regions of sequenced FLC alleles are identical, some accessions have genetically weak alleles that do not promote late flowering in the presence of FRI. In this study, a new weak allele of FLC with open reading frame identity to previously sequenced alleles was isolated from a Niederzenz (Nd) accession. …
Treefall Gaps And The Maintenance Of Species Diversity In A Tropical Forest, Stefan A. Schnitzer, Walter P. Carson
Treefall Gaps And The Maintenance Of Species Diversity In A Tropical Forest, Stefan A. Schnitzer, Walter P. Carson
Biological Sciences Faculty Research and Publications
The maintenance of species diversity by treefall gaps is a long‐standing paradigm in forest ecology. Gaps are presumed to provide an environment in which tree species of differing competitive abilities partition heterogeneous resources. The empirical evidence to support this paradigm, however, remains scarce, and some recent studies even suggest that gaps do not maintain the diversity of shade‐tolerant species. Although there is evidence that gaps maintain the diversity of pioneer trees, most of this evidence comes from studies that did not make comparisons between gaps and intact forest sites (controls). Further, nearly all studies on the maintenance of diversity by …
Oxa1p Acts As A General Membrane Insertion Machinery For Proteins Encoded By Mitochondrial Dna, Kai Hell, Walter Neupert, Rosemary A. Stuart
Oxa1p Acts As A General Membrane Insertion Machinery For Proteins Encoded By Mitochondrial Dna, Kai Hell, Walter Neupert, Rosemary A. Stuart
Biological Sciences Faculty Research and Publications
Oxa1p is a member of the conserved Oxa1/YidC/Alb3 protein family involved in the membrane insertion of proteins. Oxa1p has been shown previously to directly facilitate the export of the N‐terminal domains of membrane proteins across the inner membrane to the intermembrane space of mitochondria. Here we report on a general role of Oxa1p in the membrane insertion of proteins. (i) The function of Oxa1p is not limited to the insertion of membrane proteins that undergo N‐terminal tail export; rather, it also extends to the insertion of other polytopic proteins such as the mitochondrially encoded Cox1p and Cox3p proteins. These are …
Contributions Of Identifiable Neurons And Neuron Classes To Lamprey Vertebrate Neurobiology, James T. Buchanan
Contributions Of Identifiable Neurons And Neuron Classes To Lamprey Vertebrate Neurobiology, James T. Buchanan
Biological Sciences Faculty Research and Publications
Among the advantages offered by the lamprey brainstem and spinal cord for studies of the structure and function of the nervous system is the unique identifiability of several pairs of reticulospinal neurons in the brainstem. These neurons have been exploited in investigations of the patterns of sensory input to these cells and the patterns of their outputs to spinal neurons, but no doubt these cells could be used much more effectively in exploring their roles in descending control of the spinal cord. The variability of cell positions of neurons in the spinal cord has precluded the recognition of unique spinal …
Protransglutaminase (Factor Xiii) Mediated Crosslinking Of Fibrinogen And Fibrin, Kevin R. Siebenlist, David A. Meh, Michael W. Mosesson
Protransglutaminase (Factor Xiii) Mediated Crosslinking Of Fibrinogen And Fibrin, Kevin R. Siebenlist, David A. Meh, Michael W. Mosesson
Biological Sciences Faculty Research and Publications
No abstract provided.
Biennialism And Vernalization-Promoted Flowering In Hyoscyamus Niger: A Comparison With Arabidopsis, Michael Schläppi, Monica Patel
Biennialism And Vernalization-Promoted Flowering In Hyoscyamus Niger: A Comparison With Arabidopsis, Michael Schläppi, Monica Patel
Biological Sciences Faculty Research and Publications
There are genetic similarities between the biennial growth habit of Hyoscyamus niger (H. niger) and winter annual ecotypes of Arabidopsis thaliana, including the response to demethylating agents. One focus of our research group at Marquette is to determine whether FLOWERING LOCUS C (FLC) homologs or FLC-related MADS-box genes are involved in biennialism of H. niger. This review also summarizes our initial characterization of expression profiles of 4 groups of H. niger MADS-box genes. Our results suggest that B-class floral homeotic gene homologs of H. niger are differentially expressed in flowers of annual and …