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Prevalence, Virulence And Antibiotic Susceptibility Of Listeria Monocytogenes Isolated From Sheep, Shaimaa Elbar, Rasha Elkenany, Mohamed Elhadidy, Gamal Younis
Prevalence, Virulence And Antibiotic Susceptibility Of Listeria Monocytogenes Isolated From Sheep, Shaimaa Elbar, Rasha Elkenany, Mohamed Elhadidy, Gamal Younis
Mansoura Veterinary Medical Journal
Objective: This study was undertaken to determine the prevalence, virulence, and antibiotics susceptibility of Listeria monocytogenes isolated from hindbrain, spinal cord, milk, and intestinal content collected from sheep in the Dakahlia Governorate, Egypt.Design: Observational study.Samples: We analyzed 472 samples, including 72 hindbrain/spinal cord samples from emergency-slaughtered sheep, 300 raw-milk samples from apparently healthy sheep, and 100 intestinal content samples from slaughtered sheep at three abattoirs.Procedures: Isolation and identification of L. monocytogenes were performed using conventional techniques. The biochemically identified isolates were confirmed by 16SrRNA gene sequencing and examined for virulence-associated genes (hlyA and iap) as well as for antimicrobial susceptibility.Results: …
Expression Pattern Of Heat Shock Protein Genes In Sheep, Fawzy Younis
Expression Pattern Of Heat Shock Protein Genes In Sheep, Fawzy Younis
Mansoura Veterinary Medical Journal
Objective: To recognize the expression patterns of HSP70 and HSP90 genes in two local breeds of Sheep. Design: Descriptive study. Animals: Fifty ewes (25 Barki and 25 Abu Dlik). Procedures: This investigation was carried out on fifty sheep at the northwest coast and southeast of triangle Halayeb and Shalateen during the months of March and May 2018 and 2019 (average day time temperatures: 25–35 ◦C; relative humidity: 55–65%). Total RNA was extracted using easy-RED™ Total RNA Extraction Kit. The primers for qPCR were designed on the basis of prior sequence information available at National Center for Biotechnology Information (NCBI) for …