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Parallel Characterization Of Anaerobic Toluene-And Ethylbenzene-Degrading Microbial Consortia By Pcr-Denaturing Gradient Gel Electrophoresis, Rna-Dna Membrane Hybridization, And Dna Microarray Technology, Yoshikazu Koizumi, John J. Kelly, Tatsunori Nakagawa, Hidetoshi Urakawa, Said El Fantroussi, Saleh Al-Muzaini, Manubu Fukui, Yoshikuni Urushigawa, David A. Stahl
Parallel Characterization Of Anaerobic Toluene-And Ethylbenzene-Degrading Microbial Consortia By Pcr-Denaturing Gradient Gel Electrophoresis, Rna-Dna Membrane Hybridization, And Dna Microarray Technology, Yoshikazu Koizumi, John J. Kelly, Tatsunori Nakagawa, Hidetoshi Urakawa, Said El Fantroussi, Saleh Al-Muzaini, Manubu Fukui, Yoshikuni Urushigawa, David A. Stahl
Biology: Faculty Publications and Other Works
A mesophilic toluene-degrading consortium (TDC) and an ethylbenzene-degrading consortium (EDC) were established under sulfate-reducing conditions. These consortia were first characterized by denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR-amplified 16S rRNA gene fragments, followed by sequencing. The sequences of the major bands (T-1 and E-2) belonging to TDC and EDC, respectively, were affiliated with the family Desulfobacteriaceae. Another major band from EDC (E-1) was related to an uncultured non-sulfate-reducing soil bacterium. Oligonucleotide probes specific for the 16S rRNAs of target organisms corresponding to T-1, E-1, and E-2 were designed, and hybridization conditions were optimized for two analytical formats, membrane and …