Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 29 of 29
Full-Text Articles in Entire DC Network
Studies On Hepatitis A Virus: I. Expression Of Viral Capsid Peptides In Escherichia Coli And Their Application For An Immunoassay. Ii. Development Of An Assay For Viral Detection By Anti-Hav Antibodies., Luis Antonio Baez
LSU Historical Dissertations and Theses
A panel of eleven monoclonal antibodies of the IgG and IgM class were developed against the HM 175 strain of the Hepatitis A virus. Immunoglobulin specificity for the Hepatitis A virus was determined and the antibodies were applied toward the development of an antigen capture assay linked to a nucleic acid amplification step (RT-PCR), for detection of the Hepatitis A virus. The neutralizing mouse monoclonal antibody and a rabbit polyvalent serum were assessed as capture antibodies for the immunoaffinity stage of the assay. Fifty microliter microfuge tubes were coated with the capture antibody. Formalin inactivated HAV was diluted to concentrations …
Comparisons Of Spontaneous Mutation Rates And Estimates Of Numbers Of Cell Divisions In The Germlines Of Three Metazoans., Joni Boncille Drost
Comparisons Of Spontaneous Mutation Rates And Estimates Of Numbers Of Cell Divisions In The Germlines Of Three Metazoans., Joni Boncille Drost
LSU Historical Dissertations and Theses
Germline mutation rates measured per generation per locus in three species of Metazoa are used to address questions of the constancy of mutation rates per generation, per year and per germcell division. Null mutation rates per generation are: 3.6 $\times$ 10$\sp{-6}$ for human X-linked disease, 8.9 $\times$ 10$\sp{-6}$ for Specific Locus Test (SLT) in male mice, 3.2 $\times$ 10$\sp{-6}$ for SLT in female mice, 8.0 $\times$ 10$\sp{-6}$ for SLT in male Drosophila melanogaster (D.m.), 1.6 $\times$ 10$\sp{-6}$ for SLRL test in male D.m. and 2.0 $\times$ 10$\sp{-6}$ for SLRL test in female D.m. They were found to be very similar …
The Role Of Flagellar Proteins In Adhesion Of Vibrio Parahaemolyticus, And Isolation Of The Corresponding Gene(S)., Ratchanee Hongprayoon
The Role Of Flagellar Proteins In Adhesion Of Vibrio Parahaemolyticus, And Isolation Of The Corresponding Gene(S)., Ratchanee Hongprayoon
LSU Historical Dissertations and Theses
Polar flagellar core protein was purified from Vibrio parahaemolyticus by differential centrifugation and cesium chloride isopycnic centrifugation. Twelve hybridomas secreting monoclonal antibodies against flagellar core protein (MAb-flc) were obtained from fusions. By chance, one purified flagellar core preparation retained flagellar sheath and as a consequence, two hybridomas were detected which secreted anti-sheath antibodies (MAb-fls). MAb-flc and MAb-fls reacted specifically with their corresponding antigens as demonstrated by immunogold labelling. Coagglutination reagents prepared with MAb-flc and MAb-fls were tested against 34 strains of V. parahaemolyticus and 34 heterologous Vibrio species. The coagglutination results revealed that the flagellar core was species-specific while the …
Contributions Of Monoclonal Antibodies With Anti-Neural Cell Adhesion Molecule Like Activity To Peripheral Nerve Regeneration., Laura G. Remsen
Contributions Of Monoclonal Antibodies With Anti-Neural Cell Adhesion Molecule Like Activity To Peripheral Nerve Regeneration., Laura G. Remsen
LSU Historical Dissertations and Theses
Tubes containing monoclonal antibodies with anti-neural cell adhesion molecule (N-CAM)-like activity were applied to transected sciatic nerves to attempt to perturb the recovery of muscle function. Physiological recordings were used to estimate the return of function. The decline of implanted antibody over 28 days was estimated. No significant immune responses were detected in response to the implanted material. Electron microscopic and immunohistological analyses evaluated particular cellular disruptions in nerves due to the presence of these antibodies with anti-N-CAM like activity. Histological sections of fixed experimental nerves consistently revealed abnormal gaps between Schwann cells of regenerating nerves. This specific Schwann cell …
Genetic Population Structure Of A Species Complex Of Blue Mussels (Mytilus Spp.)., Shane Kevin Sarver
Genetic Population Structure Of A Species Complex Of Blue Mussels (Mytilus Spp.)., Shane Kevin Sarver
LSU Historical Dissertations and Theses
Mussels representing two species (Mytilus trossulus and M. galloprovincialis) were collected from 28 littoral locations on the Pacific coast of the USA, and examined for genetic variation at 15 allozyme loci. Eleven samples from a region of suspected hybridization between the two species were also analyzed for variation in seven shell characters. Analysis of allozyme data by principal components analysis revealed three groups based on first principal component scores, and were identified as M. trossulus, M. galloprovincialis and hybrids. Canonical discriminate analysis of seven shell characters was less successful in separating mussels based on morphological criteria alone. To explore the …
Interaction Between Hydrodynamics And Sediment Topography On Meiofaunal Abundance And Distribution., Bin Sun
Interaction Between Hydrodynamics And Sediment Topography On Meiofaunal Abundance And Distribution., Bin Sun
LSU Historical Dissertations and Theses
The objective of this dissertation was to investigate the small-scale spatial and abundance patterns of meiofauna and their relationship with sediment microtopography and hydrodynamics. Meiofauna are known to be aggregated at small spatial scales. A study was first undertaken to determine, how patchiness differed over seasons and habitats. Significant small-scale aggregations were remarkably consistent across different seasons and habitats for all abundant copepods of an intertidal mudflat. Spatial autocorrelation analysis showed that the abundance of copepods was autocorrelated at short distances (cm scale) on all sample collecting dates, indicating a consistent spatial pattern. Small-scale variation contributed heavily to the components …
Studies On The Energy Mobilizing Hormones In Insects., Subrata Das
Studies On The Energy Mobilizing Hormones In Insects., Subrata Das
LSU Historical Dissertations and Theses
Chemical factors controlling the energy mobilization in three species of insects were identified, partially characterized and their role in the process of energy mobilization were studied. High performance liquid chromatographic fractions were isolated from methanolic extracts of the corpora cardiaca (CC) from the honeybee Apis mellifera, the paper wasp Polistes exclamans and the house cricket Acheta domesticus. Each species had a single active fraction that was able to elevate the lipid titer in Acheta. Pyroglutamate aminopeptidase digestion of isolated active fractions indicated that these molecules are all peptides with a terminal pyroglutamate. Methanolic extracts of CC from Apis, Polistes and …
Physiological And Biochemical Responses To Hypoxia In The Blue Crab, Callinectes Sapidus Rathbun, The Lesser Blue Crab, Callinectes Similis Williams, And The Southern Oyster Drill, Stramonita Haemastoma Linnaeus., Tapash Das
LSU Historical Dissertations and Theses
This study measured the physiological and biochemical changes associated with exposure of the juvenile blue crab, Callinectes sapidus Rathbun and the lesser blue crab. C. similis Williams, to long term (28 d) hypoxia, short term (10 d) transfer from hypoxia to normoxia and a diurnally fluctuating oxygen regime for 28 days. The southern oyster drill, Stramonita haemastoma Linnaeus, was also exposed to 28 days of constant hypoxia to compare the responses of a tolerant species with the two species of Callinectes. The 28 day LC$\sb{50}$ estimates for C. sapidus, C. similis and S. haemastoma were respectively 106, 43 and 11.5 …
Structural Analysis And Methionine Ehancement Of The Bean Seed Storage Protein Phaseolin., John M. Dyer
Structural Analysis And Methionine Ehancement Of The Bean Seed Storage Protein Phaseolin., John M. Dyer
LSU Historical Dissertations and Theses
Common beans are widely utilized as a food source, yet are limited as a complete source of protein due to low levels of methionine, an essential amino acid for humans. A protein engineering strategy was developed to increase the methionine content of phaseolin, the primary seed storage protein in common beans. The engineering strategy consists of three major parts. In the first part, a set of biophysical probes was developed to characterize the stability of wild-type and modified phaseolin proteins. I used absorbance, fluorescence emission, circular dichroism, and fluorescence polarization anisotropy to monitor phaseolin denaturation induced by urea, guanidinium chloride, …
Functional Morphology Of The Lingual Apparatus Of Sandgrouse (Aves: Pteroclidae)., Werner Muller
Functional Morphology Of The Lingual Apparatus Of Sandgrouse (Aves: Pteroclidae)., Werner Muller
LSU Historical Dissertations and Theses
The morphology of the lingual apparatus of three species of sandgrouse (Aves; Pteroclidae) has been described. All biomechanically important parts were included in the description, i.e., all muscles potentially involved in the functioning of the lingual apparatus, all skeletal parts, the salivary glands associated with the lingual apparatus, and the surface morphology of the oropharyngeal cavity. For each muscle its potential functions have been described. In a comparison with data from the literature of three other groups of birds it was found that the functional organization of the lingual apparatus of the sandgrouse is strikingly similar to the one found …
Strongylus Vulgaris: Protection, Pathology And Humoral Immune Responses In The Immunized Pony., Clifton Michael Monahan
Strongylus Vulgaris: Protection, Pathology And Humoral Immune Responses In The Immunized Pony., Clifton Michael Monahan
LSU Historical Dissertations and Theses
Protective resistance to Strongylus vulgaris infection was examined by immunization of ponies with radiation-attenuated larvae or soluble parasite homogenates. Twelve yearling ponies raised and maintained under parasite-free conditions were divided into four groups which received either radiation-attenuated L$\sb3$; aqueous-soluble adult somatic antigens plus adjuvant; aqueous-soluble larval somatic antigens with excretory/secretory products and adjuvant; or media with adjuvant. Ponies were immunized twice; radiation-attenuated L$\sb3$ were administered orally and soluble antigens or control injections were given intramuscularly. Six weeks following the second immunization, ponies were challenged per os with virulent S. vulgaris L$\sb3.$ Ponies were monitored twice daily for rectal temperatures and …
Photosynthetic Response To Elevated Carbon Dioxide Concentrations In The Aerenchyma Of Typha Latifolia L. Leaves., John Van Horne Constable
Photosynthetic Response To Elevated Carbon Dioxide Concentrations In The Aerenchyma Of Typha Latifolia L. Leaves., John Van Horne Constable
LSU Historical Dissertations and Theses
This study examined the importance of the leaf aerenchyma gas space as a CO$\sb2$ source for photosynthesis in Typha latifolia L. (broadleaf cattail). In the field there was a distinct diurnal pattern of CO$\sb2$ concentration ( (CO$\sb2$)) in the aerenchyma gas space. At dawn the aerenchyma (CO$\sb2$) was 4 to 18 times above atmospheric levels. By midday the aerenchyma (CO$\sb2$) declined to near atmospheric levels and increased again in the late afternoon. It is hypothesized that this diurnal pattern may be controlled by photosynthetic demand for CO$\sb2$. Aerenchyma gas space was estimated as $>$50% of leaf volume, and the …
Circadian Neuroendocrine Regulation Of Scale Allograft Reactions In Gulf Killifish, Fundulus Grandis., Nicholas John Nevid
Circadian Neuroendocrine Regulation Of Scale Allograft Reactions In Gulf Killifish, Fundulus Grandis., Nicholas John Nevid
LSU Historical Dissertations and Theses
Reactions against scale allografts in gulf killifish were studied to examine circadian variation and neuroendocrine regulation of immune function. Although melanophores in autografts remain viable indefinitely, melanophores in scale allografts were broken down predominantly at night (12-h scotophases) regardless of the time of transplantation. Daily treatment with hormones or antagonists of neuroendocrine receptors altered the rhythm of melanophore breakdown when administered at light onset or light offset. Daily rhythms of immune activity may be a direct reflection of growth hormone levels and are entrained by exogenous cortisol treatment. Daily nonphotoperiodic environmental stimuli overrode photoperiodic entrainment of immune activity. Peak melanophore …
Resolution Of Genotypic And Phenotypic Properties Of Herpes Simplex Virus Type 1 Temperature-Sensitive Mutanttsz47: Evidence For Allelic Complementation In The Unique Long Gene Ul28., James Daniel Cavalcoli
Resolution Of Genotypic And Phenotypic Properties Of Herpes Simplex Virus Type 1 Temperature-Sensitive Mutanttsz47: Evidence For Allelic Complementation In The Unique Long Gene Ul28., James Daniel Cavalcoli
LSU Historical Dissertations and Theses
This research focuses on the phenotypic and genotypic characteristics of a temperature sensitive mutant of herpes simplex virus type 1, HSV-1 (tsZ47). This virus was previously reported to carry two different mutations, which caused it to be temperature sensitive for viral replication and transport of glycoproteins to the infected cell surface. However, altered glycoprotein transport to the cell surface was not observed in this study. In addition, cells transformed with unique long gene 28 (UL28), but not glycoprotein B (gB), complemented tsZ47 at the nonpermissive temperature indicating that a single mutation could restore the wild type phenotype to the mutant …
The Phylogeny Of The Prasinophyceae And Pleurastrophyceae (Chlorophyta) Inferred From Ribosomal Rna Genes And Morphology., Thomas Sinclair Kantz
The Phylogeny Of The Prasinophyceae And Pleurastrophyceae (Chlorophyta) Inferred From Ribosomal Rna Genes And Morphology., Thomas Sinclair Kantz
LSU Historical Dissertations and Theses
The phylogeny of the problematic green algal classes Prasinophyceae and Pleurastrophyceae (Chlorophyta) sensu Mattox and Stewart (1984) was inferred from ultrastructural and biochemical data, and from ribosomal RNA gene sequence data. Analysis of a data set of ultrastructural and biochemical characters (with a predominance of flagellar and cell covering features) revealed a monophyletic Pleurastrophyceae and a monophyletic Prasinophyceae. Rerooting experiments indicated that the ancestral flagellate was likely Pedinomonas-like or Mamiellales-like. The cladograms generated from the ribosomal RNA gene sequence data show that the Pleurastrophyceae is not monophyletic; however, pleurastrophycean taxa are more closely related to the Chlorophyceae than to any …
Characterization Of Physiological Responses Of Suspension Cells From Alligator Weed (Alternanthera Philoxeroides (Mart.); Griseb) To An Abrupt Increase In Salinity., Gloria Balagtas-Burow
Characterization Of Physiological Responses Of Suspension Cells From Alligator Weed (Alternanthera Philoxeroides (Mart.); Griseb) To An Abrupt Increase In Salinity., Gloria Balagtas-Burow
LSU Historical Dissertations and Theses
Dark-grown suspension cells of alligator weed (Alternanthera philoxeroides (Mart.) Griseb) were established in Murashige and Skoog medium and used to characterize several cellular responses to an abrupt increase in salinity (0 to 200 mol m$\sp{-3}$ NaCl). The fresh weight and cell density of salt-treated cells of alligator weed more than doubled by 7 d. Cell viability declined to 77% by 1 d, but recovered to 86% by 7 d after salt treatment. These responses indicate that alligator weed cells survived and adapted to an abrupt increase in salinity. Osmotic potential ($\psi\sb\pi$) of salt-treated cells declined to the $\psi\sb\pi$ of the …
The Genetic Effects Of Forest Fragmentation On Amazonian Forest Birds., John Marshall Bates
The Genetic Effects Of Forest Fragmentation On Amazonian Forest Birds., John Marshall Bates
LSU Historical Dissertations and Theses
I present allozyme and mitochondrial DNA (mtDNA) data on the genetic structure of five species of Amazonian forest birds at two geographic scales. The five species, Glyphorynchus spirurus (family Dendrocolaptidae), Leptopogon amaurocephalus (Tyrannidae), Hypocnemis cantator, Myrmeciza hemimelaena, and Hylophylax poecilinota (all three Formicariidae), are common forest understory inhabitants throughout much of Amazonia. At the local geographic scale, I hypothesized that forest fragmentation affected genetic structure in these species. Study sites were in the Department of Santa Cruz, Bolivia. Six sites separated by 150 km or less for each species were sampled: three sites in continuous Amazonian forest, and three in …
Spectra Of Mutations Induced By Tritium Beta Radiation Or 2-Chloroethyl Methanesulfonate In Drosophila Melanogaster Germ Cells., Nancy G. Fossett
Spectra Of Mutations Induced By Tritium Beta Radiation Or 2-Chloroethyl Methanesulfonate In Drosophila Melanogaster Germ Cells., Nancy G. Fossett
LSU Historical Dissertations and Theses
Tritium beta radiation (tritium) was used to induce mutations at the alcohol dehydrogenase (Adh) locus in male Drosophila melanogaster post-meiotic germ cells. All 23 mutations recovered were deletions greater than one kilobase in size (multikilobase deletions) as determined by genetic complementation analysis. A statistical difference was observed between the frequency of multikilobase deletions (23/23 or 1.0) induced by tritium and the frequency of multikilobase deletions (19/27 or 0.7) induced by 100-kVp X-radiation (X-rays). The relative frequency of multikilobase deletions induced by tritium with respect to that induced by X-rays was 1.4. This value was compared to the relative biological effectiveness …
In Vitro Studies On The Interactions Between Toxoplasma Gondii And Its Host Cell., Sandra Karen Halonen
In Vitro Studies On The Interactions Between Toxoplasma Gondii And Its Host Cell., Sandra Karen Halonen
LSU Historical Dissertations and Theses
Toxoplasma gondii is an obligate intracellular protozoan parasite of the Phylum Apicomplexa. The parasite resides in the cytoplasm of its host cells in a membrane bound compartment called the parasitophorous vacuole. All growth and development of the parasite occur within this compartment. In this thesis, the interactions between the Vero cells, a fibroblast-like cell line, and the parasite which occur during the intracellular growth of the parasite were addressed. The impact of infection on the host cell intermediate filaments and microtubular cytoskeletal elements was examined by immunofluorescence microscopy. Host cell intermediate filaments were found to overcoat the parasite vacuole beginning …
Acetylation Of Seaweed Alginate By Pseudomonas Syringae., Jin Woo Lee
Acetylation Of Seaweed Alginate By Pseudomonas Syringae., Jin Woo Lee
LSU Historical Dissertations and Theses
Acetylation of bacterial alginate by Pseudomonas syringae subsp. phaseolicola ATCC 19304 was independent of alginate biosynthesis. This allowed the development of a process for acetylating seaweed alginate using immobilized P. syringae ATCC 19304 cells. About 50% of the mannuronic acid residues of seaweed alginate were acetylated by carbon immobilized P. syringae cells in a fluidized bed, up-flow reactor system fed continuously with seaweed alginate and gluconic acid. O-Acetylation by this process was found to be specific for the C-2 and/or C-3 position(s) of mannuronate residues. Acetylated seaweed alginate showed altered properties including increased viscosity and changed affinities for some cations.
Determinants Of Mangrove Species Distribution In Neotropical Forests: Biotic And Abiotic Factors Affecting Seedling Survival And Growth., Karen Lee Mckee
Determinants Of Mangrove Species Distribution In Neotropical Forests: Biotic And Abiotic Factors Affecting Seedling Survival And Growth., Karen Lee Mckee
LSU Historical Dissertations and Theses
Mangroves are salt-tolerant trees and shrubs that dominate the intertidal zone in many tropical and subtropical coastal regions. These intertidal forests often exhibit relatively distinct and recurring patterns of species distribution. A series of field and laboratory experiments examined biotic and abiotic factors affecting survival and growth of three neotropical mangrove species: Rhizophora mangle (red mangrove), Avicennia germinans (black mangrove), and Laguncularia racemosa (white mangrove). A field survey across a mangrove island range in Belize revealed six vegetation zones based on differences in dominant canopy species, size and vigor of the trees, spatial position relative to the shoreline, and hydro-edaphic …
Soil Processes Related To Marsh Loss In Coastal Louisiana., John Andrew Nyman
Soil Processes Related To Marsh Loss In Coastal Louisiana., John Andrew Nyman
LSU Historical Dissertations and Theses
Marsh loss in Louisiana ($\sim$8,000 ha/yr) is attributed primarily to salt-water intrusion, flooding, and sediment starvation. Soil processes were studied to complement previous work identifying causes rather than mechanisms. Relationships between soil mineral and organic matter were examined to better understand accretion, which counters subsidence and sea-level rise. More soil volume was occupied by organic matter than mineral matter in fresh, intermediate, and brackish soil. Estimated mineral sediment requirements for accretion were 6.7 times greater in salt marsh than in fresh marsh. Sediment requirements probably increase seaward because of sulfate in seawater, reduction of sulfate to toxic sulfides in soil, …
Transcription Initiation Studies With Bacillus Subtilis Promoters Containing Curved Dna., Douglas James Stemke
Transcription Initiation Studies With Bacillus Subtilis Promoters Containing Curved Dna., Douglas James Stemke
LSU Historical Dissertations and Theses
Many promoters contain a curved DNA component essential for high levels of transcription activity. The present work approached the study of these unusual DNA structures in a twofold manner. First, promoter binding by RNA polymerase from Bacillus subtilis and Escherichia coli was examined using a collection of promoters containing DNA curvature. Promoter binding by both RNA polymerases was governed primarily by the nucleotide sequence at the highly conserved $-$10 and $-$35 regions of the promoters. However, the presence of curved DNA, immediately upstream of the $-$35 region of the promoter, predictably increased binding to those promoters which contained curved DNA …
Sulfadimethoxine Analysis In Channel Catfish: A Model Drug Residue Monitoring Program For Aquaculture., Calvin Charles Walker
Sulfadimethoxine Analysis In Channel Catfish: A Model Drug Residue Monitoring Program For Aquaculture., Calvin Charles Walker
LSU Historical Dissertations and Theses
Analytical methods for extraction, detection, and quantitation of sulfadimethoxine (SDM) residues in channel catfish muscle and plasma were developed for a tiered residue monitoring program. The plasma and muscle concentrations of SDM and its primary metabolite in channel catfish, 4-N-acetylsulfadimethoxine (N-acetyl SDM), were determined in SDM medicated fish. Drug extraction methods using matrix solid phase dispersion (MSPD), drug screening methods using enzyme-linked immunoassay (ELISA), and drug residue quantitation methods using high pressure liquid chromatography (LC) are presented. All methods were developed for the simultaneous extraction and analysis of the parent compound and metabolite. MSPD extracts of muscle or plasma were …
Characterization Of The Subunits Of The Mcrbc Restriction System In Escherichia Coli K12., Timothy Paul Beary
Characterization Of The Subunits Of The Mcrbc Restriction System In Escherichia Coli K12., Timothy Paul Beary
LSU Historical Dissertations and Theses
The McrBC (Modified Cytosine Restriction) restriction system has the ability to restrict DNA containing 5-hydroxymethylcytosine, N$\sp4$-methylcytosine, and 5-methylcytosine at specific sequences. The mcrB gene produces two gene products. The complete mcrB open reading frame produces a 53-kDa protein (McrB$\sb{\rm L}$) and a 35-kDa protein (McrB$\sb{\rm S}).$ The smaller McrB polypeptide is produced from an inframe, internal translational start in the mcrB gene. The mcrC gene produces a single 38-kDa protein. Evidence was presented that McrB$\sb{\rm S}$ regulates the activity of McrBC. When McrB$\sb{\rm S}$ was overproduced in a McrBC$\sp+$ background, there was dramatic loss of restriction. Underproduction of this protein …
Seed Dormancy In Red Rice (Oryza Sativa): Changes In Embryo Ph And Metabolism During The Dormancy-Breaking Process., Steven Footitt
Seed Dormancy In Red Rice (Oryza Sativa): Changes In Embryo Ph And Metabolism During The Dormancy-Breaking Process., Steven Footitt
LSU Historical Dissertations and Theses
Hydrated, dehulled, dormant seeds of red rice (Oryza sativa L.) were exposed to chemical treatments (nitrite, propionic acid, methyl propionate, propionaldehyde, and n-propanol) that saturate the dormancy-breaking response. Dormancy-breaking chemicals were (a) metabolized (n-propanol and propionate) by embryos in dormant seeds to weak acids; (b) decreased embryo pH; and (c) increased embryo (Fru 2,6-P$\sb2$) prior to dormancy-breaking. Embryo acidification, but not increased embryo (Fru 2,6-P$\sb2$), was associated with the chemical contact interval required for the onset of dormancy-breaking. During chemical contact, embryo (Fru 2,6-P$\sb2$) increased independent of dormancy-breaking and was inversely correlated with the elapsed time to 30% germination. On …
Immunologic And Pathologic Responses Of Macaca Mulatta Experimentally Infected With Simian Immunodeficiency Virus And Campylobacter Jejuni., Robert Wayne Kornegay
Immunologic And Pathologic Responses Of Macaca Mulatta Experimentally Infected With Simian Immunodeficiency Virus And Campylobacter Jejuni., Robert Wayne Kornegay
LSU Historical Dissertations and Theses
Five Macaca mulatta were infected with SIV/DELTA$\sb{\rm B670}.$ Approximately 70 days later, this group (SIV) and five age, sex, weight matched not SIV-infected (NEG) M. mulatta were inoculated orally with approximately $3\times10\sp{10}$ Campylobacter jejuni. Small and large bowel mucosal biopsies, bowel contents, sera, blood, and rectal swabs were collected before and after C. jejuni inoculation. Biopsy cryosections were stained with histologic stains and immunocytochemically for lymphocyte subsets, plasmacytes, macrophages, and B-cells. Coded stained sections were examined semiquantitatively or quantitatively using computerized image analysis. Circulating lymphocyte subsets were determined using FACS analysis. Campylobacter jejuni was recultured from 4/5 SIV and 4/5 …
Factors Contributing To Resistance In Rice To The Rice Water Weevil, Lissorhoptrus Oryzophilus Kuschel., Francois Kouame N'Guessan
Factors Contributing To Resistance In Rice To The Rice Water Weevil, Lissorhoptrus Oryzophilus Kuschel., Francois Kouame N'Guessan
LSU Historical Dissertations and Theses
Studies were conducted in the field and greenhouse to identify sources of rice water weevil (Lissorhoptrus oryzophilus Kuschel) resistance in rice, Oryza sativa L., and determine the mechanisms of resistance. Rice lines of various sources, including breeding lines, somaclone lines and world collection lines, were evaluated. Tolerance was investigated using replicated insecticide treated and untreated plots in a split-plot design, and antibiosis and/or antixenosis were assessed using caged insect-infested plants in randomized block designs. Resistance predictors included rice water weevil larval populations, larval root pruning damage, plant height and grain yield. Anther culture lines 95-2836 and 95-3527, Louisiana breeding lines …
Cultural Tactics For Control Of The Rice Water Weevil, Lissorhoptrus Oryzophilus Kuschel., Reginald Alexander Thompson
Cultural Tactics For Control Of The Rice Water Weevil, Lissorhoptrus Oryzophilus Kuschel., Reginald Alexander Thompson
LSU Historical Dissertations and Theses
A series of field studies were conducted from 1990 to 1992, designed specifically to identify cultural tactics that may be used to control the rice water weevil, Lissorhoptrus oryzophilus Kuschel, on rice (Oryza sativa L.). The objectives of the studies were: to examine the effects of plant density on rice water weevil oviposition, to compare carbofuran and drainage as rice water weevil control tactics and to examine the impact of 1-3 days delay in reflooding after drainage on rice yields, and to assess the impact of planting date on rice water weevil larval damage. The results of the plant density …