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Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco (Nicotiana Tabacum L.) Leaf Disks: Evaluation Of The Co-Cultivation Conditions To Increase Beta-Glucuronidase Gene Activity, Sunjung Park
LSU Master's Theses
Agrobacterium tumefaciens-mediated transformation is generally used for genetic transformation of higher plants. Several experimental factors important for the increase of beta-glucuronidase (GUS) reporter gene activity were evaluated in this study using leaf disks of tobacco (Nicotiana tabacum L. cv. Xanthi). We found that co-cultivation temperature at 20C is the most critical factor to obtain the reproducible enhancement of GUS activity. pCAMBIA 1305.01 resulted in higher GUS activity than the other two pCAMBIA vectors 1301 and 1305.02. The highest GUS activity and transformation efficiency were achieved under the following experimental conditions: Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA1305.01 was grown overnight at …