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Isolation And Characterization Of Bacteria In A Toluene-Producing Enrichment Culture Derived From Contaminated Groundwater At A Louisiana Superfund Site, Madison Mikes

LSU Master's Theses

In an effort to better understand the role that various microbes may play in toluene production, bacteria from a toluene-producing enrichment culture derived from contaminated groundwater at a Superfund site were cultivated and isolated on low nutrient solid media. A total of 14 solid medium formulations containing varying pH ranges, carbon sources, solidifying agents, and incubation gas headspaces were used to obtain 278 isolates in pure culture. Isolated bacteria, identified using partial 16S rRNA gene sequences, were most closely related with the genera Anoxybacillus, Azospira, Bacillus, Bradyrhizobium, Cellulosimicrobium, Micrococcus, and Propionicimonas.

Further attempts …

Antibiotic Resistance In Bacteria From Songbirds And Fitness Studies Following Environmental Stressors In Vitro, Leah R. Forsyth

LSU Master's Theses

Since their initial discovery in 1928, antibiotics have been utilized for agriculture and human health purposes. The increasing use of antibiotics by humans and the rapid evolution of antibiotic resistance might be leading to the presence of more antibiotic-resistant bacteria in the environment. Understanding potential relationships between humans, bacteria, and the environment is important in order to study the spread and progression of antibiotic resistance in bacteria. Several past studies have focused on oxidative stress, temperature stress, and antibiotic resistance in bacteria, but to my knowledge few if any have done a wide comparison between these environmental stressors and the …