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Specificity Of Chaperonin Groel Binding To The Precursor Of The Small Subunit Of Ribulose-1,5-Bisphosphate Carboxylase., Carmen Wheelock Dessauer
Specificity Of Chaperonin Groel Binding To The Precursor Of The Small Subunit Of Ribulose-1,5-Bisphosphate Carboxylase., Carmen Wheelock Dessauer
LSU Historical Dissertations and Theses
Although chaperonin-assisted protein folding has been studied in vitro by a number of investigators, the features of an unfolded or partially folded polypeptide that are recognized and bound by chaperonins are not known. I addressed this question using the precursor of the small subunit (pS) of ribulose-1,5-bisphosphate carboxylase as a model substrate for GroEL, the bacterial chaperonin. The precursor protein was expressed in E. coli as a C-terminal fusion to protein A. Protein A-pS (and any associated cellular proteins) was isolated by affinity chromatography. GroEL could be eluted from the fusion protein by ATP and either GroES or casein, consistent …
Photosynthetic Response To Elevated Carbon Dioxide Concentrations In The Aerenchyma Of Typha Latifolia L. Leaves., John Van Horne Constable
Photosynthetic Response To Elevated Carbon Dioxide Concentrations In The Aerenchyma Of Typha Latifolia L. Leaves., John Van Horne Constable
LSU Historical Dissertations and Theses
This study examined the importance of the leaf aerenchyma gas space as a CO$\sb2$ source for photosynthesis in Typha latifolia L. (broadleaf cattail). In the field there was a distinct diurnal pattern of CO$\sb2$ concentration ( (CO$\sb2$)) in the aerenchyma gas space. At dawn the aerenchyma (CO$\sb2$) was 4 to 18 times above atmospheric levels. By midday the aerenchyma (CO$\sb2$) declined to near atmospheric levels and increased again in the late afternoon. It is hypothesized that this diurnal pattern may be controlled by photosynthetic demand for CO$\sb2$. Aerenchyma gas space was estimated as $>$50% of leaf volume, and the …
Characterization Of Physiological Responses Of Suspension Cells From Alligator Weed (Alternanthera Philoxeroides (Mart.); Griseb) To An Abrupt Increase In Salinity., Gloria Balagtas-Burow
Characterization Of Physiological Responses Of Suspension Cells From Alligator Weed (Alternanthera Philoxeroides (Mart.); Griseb) To An Abrupt Increase In Salinity., Gloria Balagtas-Burow
LSU Historical Dissertations and Theses
Dark-grown suspension cells of alligator weed (Alternanthera philoxeroides (Mart.) Griseb) were established in Murashige and Skoog medium and used to characterize several cellular responses to an abrupt increase in salinity (0 to 200 mol m$\sp{-3}$ NaCl). The fresh weight and cell density of salt-treated cells of alligator weed more than doubled by 7 d. Cell viability declined to 77% by 1 d, but recovered to 86% by 7 d after salt treatment. These responses indicate that alligator weed cells survived and adapted to an abrupt increase in salinity. Osmotic potential ($\psi\sb\pi$) of salt-treated cells declined to the $\psi\sb\pi$ of the …
Determinants Of Mangrove Species Distribution In Neotropical Forests: Biotic And Abiotic Factors Affecting Seedling Survival And Growth., Karen Lee Mckee
Determinants Of Mangrove Species Distribution In Neotropical Forests: Biotic And Abiotic Factors Affecting Seedling Survival And Growth., Karen Lee Mckee
LSU Historical Dissertations and Theses
Mangroves are salt-tolerant trees and shrubs that dominate the intertidal zone in many tropical and subtropical coastal regions. These intertidal forests often exhibit relatively distinct and recurring patterns of species distribution. A series of field and laboratory experiments examined biotic and abiotic factors affecting survival and growth of three neotropical mangrove species: Rhizophora mangle (red mangrove), Avicennia germinans (black mangrove), and Laguncularia racemosa (white mangrove). A field survey across a mangrove island range in Belize revealed six vegetation zones based on differences in dominant canopy species, size and vigor of the trees, spatial position relative to the shoreline, and hydro-edaphic …