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Solid-State Nmr Of Unlabeled Plant Cell Walls: High-Resolution Structural Analysis Without Isotopic Enrichment, Wancheng Zhao, Alex Kirui, Fabien Deligey, Frederic Mentink-Vigier, Yihua Zhou, Baocai Zhang, Tuo Wang
Solid-State Nmr Of Unlabeled Plant Cell Walls: High-Resolution Structural Analysis Without Isotopic Enrichment, Wancheng Zhao, Alex Kirui, Fabien Deligey, Frederic Mentink-Vigier, Yihua Zhou, Baocai Zhang, Tuo Wang
Faculty Publications
© 2021, The Author(s). Background: Multidimensional solid-state nuclear magnetic resonance (ssNMR) spectroscopy has emerged as an indispensable technique for resolving polymer structure and intermolecular packing in primary and secondary plant cell walls. Isotope (13C) enrichment provides feasible sensitivity for measuring 2D/3D correlation spectra, but this time-consuming procedure and its associated expenses have restricted the application of ssNMR in lignocellulose analysis. Results: Here, we present a method that relies on the sensitivity-enhancing technique Dynamic Nuclear Polarization (DNP) to eliminate the need for 13C-labeling. With a 26-fold sensitivity enhancement, a series of 2D 13C–13C correlation spectra were successfully collected using the unlabeled …
Solid-State Nmr Of Plant And Fungal Cell Walls: A Critical Review, Wancheng Zhao, Liyanage D. Fernando, Alex Kirui, Fabien Deligey, Tuo Wang
Solid-State Nmr Of Plant And Fungal Cell Walls: A Critical Review, Wancheng Zhao, Liyanage D. Fernando, Alex Kirui, Fabien Deligey, Tuo Wang
Faculty Publications
© 2020 Elsevier Inc. The cell walls of plants and microbes are a central source for bio-renewable energy and the major targets of antibiotics and antifungal agents. It is highly challenging to determine the molecular structure of complex carbohydrates, protein and lignin, and their supramolecular assembly in intact cell walls. This article selectively highlights the recent breakthroughs that employ 13C/15N solid-state NMR techniques to elucidate the architecture of fungal cell walls in Aspergillus fumigatus and the primary and secondary cell walls in a large variety of plant species such as Arabidopsis, Brachypodium, maize, and spruce. Built upon these pioneering studies, …
Atomic Resolution Of Cotton Cellulose Structure Enabled By Dynamic Nuclear Polarization Solid-State Nmr, Alex Kirui, Zhe Ling, Xue Kang, Malitha C. Dickwella Widanage, Frederic Mentink-Vigier, Alfred D. French, Tuo Wang
Atomic Resolution Of Cotton Cellulose Structure Enabled By Dynamic Nuclear Polarization Solid-State Nmr, Alex Kirui, Zhe Ling, Xue Kang, Malitha C. Dickwella Widanage, Frederic Mentink-Vigier, Alfred D. French, Tuo Wang
Faculty Publications
© 2018, Springer Nature B.V. Abstract: The insufficient resolution of conventional methods has long limited the structural elucidation of cellulose and its derivatives, especially for those with relatively low crystallinities or in native cell walls. Recent 2D/3D solid-state NMR studies of 13 C uniformly labeled plant biomaterials have initiated a re-investigation of our existing knowledge in cellulose structure and its interactions with matrix polymers but for unlabeled materials, this spectroscopic method becomes impractical due to limitations in sensitivity. Here, we investigate the molecular structure of unlabeled cotton cellulose by combining natural abundance 13 C– 13 C 2D correlation solid-state NMR …
Structural Factors Affecting 13C Nmr Chemical Shifts Of Cellulose: A Computational Study, Hui Yang, Tuo Wang, Daniel Oehme, Loukas Petridis, Mei Hong, James D. Kubicki
Structural Factors Affecting 13C Nmr Chemical Shifts Of Cellulose: A Computational Study, Hui Yang, Tuo Wang, Daniel Oehme, Loukas Petridis, Mei Hong, James D. Kubicki
Faculty Publications
© 2017, The Author(s). The doublet C4 peaks at ~ 85 and ~ 89 ppm in solid-state 13C NMR spectra of native cellulose have been attributed to signals of C4 atoms on the surface (solvent-exposed) and in the interior of microfibrils, designated as sC4 and iC4, respectively. The relative intensity ratios of sC4 and iC4 observed in NMR spectra of cellulose have been used to estimate the degree of crystallinity of cellulose and the number of glucan chains in cellulose microfibrils. However, the molecular structures of cellulose responsible for the specific surface and interior C4 peaks have not been positively …
2H–13C Correlation Solid-State Nmr For Investigating Dynamics And Water Accessibilities Of Proteins And Carbohydrates, Martin D. Gelenter, Tuo Wang, Shu Yu Liao, Hugh O’Neill, Mei Hong
2H–13C Correlation Solid-State Nmr For Investigating Dynamics And Water Accessibilities Of Proteins And Carbohydrates, Martin D. Gelenter, Tuo Wang, Shu Yu Liao, Hugh O’Neill, Mei Hong
Faculty Publications
© 2017, Springer Science+Business Media B.V. Site-specific determination of molecular motion and water accessibility by indirect detection of 2H NMR spectra has advantages over dipolar-coupling based techniques due to the large quadrupolar couplings and the ensuing high angular resolution. Recently, a Rotor Echo Short Pulse IRrAdiaTION mediated cross polarization (RESPIRATIONCP) technique was developed, which allowed efficient transfer of 2H magnetization to 13C at moderate 2H radiofrequency field strengths available on most commercial MAS probes. In this work, we investigate the 2H–13C magnetization transfer characteristics of one-bond perdeuterated CDn spin systems and two-bond H/D exchanged C–(O)–D and C–(N)–D spin systems in …
Multidimensional Solid-State Nmr Spectroscopy Of Plant Cell Walls, Tuo Wang, Pyae Phyo, Mei Hong
Multidimensional Solid-State Nmr Spectroscopy Of Plant Cell Walls, Tuo Wang, Pyae Phyo, Mei Hong
Faculty Publications
© 2016 Elsevier Inc. Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is uniquely suited for studying native hydrated plant cell walls at the molecular level with chemical resolution. Significant progress has been made in the last five years to elucidate the molecular structures and interactions of cellulose and matrix polysaccharides in plant cell walls. These studies have focused on primary cell walls of growing …