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The Dextranase Of Lipomyces Starkeyi And Its Use In Sugar Cane Processing., David William Koenig
The Dextranase Of Lipomyces Starkeyi And Its Use In Sugar Cane Processing., David William Koenig
LSU Historical Dissertations and Theses
Dextranase is an enzyme which destroys dextran, a contaminant in sugar and a main constituent of dental plaque. Dextranase utilization can solve a major economic problem in the sugar industry, and in dental preparations it can keep teeth essentially free of plaque. The dextranase from the yeast Lipomyces starkeyi was investigated because this organism has been used in food related processes. This attribute should allow for Food and Drug Administration (FDA) approval for use of a Lipomyces dextranase. None of the existing commercial dextranase preparations have FDA approval. L. starkeyi was found to produce maximum amounts of dextranase when grown …
A Molecular Characterization Of An Escherichia Coli Restriction System Specific For 5-Methylcytosine-Containing Dna., Troy Kevin Ross
A Molecular Characterization Of An Escherichia Coli Restriction System Specific For 5-Methylcytosine-Containing Dna., Troy Kevin Ross
LSU Historical Dissertations and Theses
The McrB restriction system in Escherichia coli K-12 is responsible for sequence-specific recognition and inactivation of DNA containing 5-methylcytosine. A derivative of plasmid pUC8 with a 5.5-kilobase pair BglII-EcoRI restriction fragment from the E. coli K-12 chromosome, imparted the wild-type phenotype to the McrB$\sp-$ strain K802. The limits of the McrB region within this DNA fragment were defined by deleting portions of the 5.5-kb insert and assaying for McrB restriction of M.AluI-methylated DNA. Analyses of polypeptides encoded by the McrB region using a maxicell strain revealed that at least three proteins, having molecular weights of approximately 51,000, 39,000, and 33,000, …
Production And Evaluation Of Rapid Serological Detection Methods For Identification Of Vibrio Vulnificus And Vibrio Cholerae., Janet Gibson Simonson
Production And Evaluation Of Rapid Serological Detection Methods For Identification Of Vibrio Vulnificus And Vibrio Cholerae., Janet Gibson Simonson
LSU Historical Dissertations and Theses
Species within the genus Vibrio can be identified serologically through detection of species specific H antigens expressed in the core protein of the polar flagella. Cholera vibrios also exhibit a specific cell wall polysaccharide antigen (A). Antibody reactive with these specific antigens can be employed for the rapid serological identification of Vibrio isolates. Species-specific anti-H sera were produced in rabbits immunized with flagellar core protein prepared from V. vulnificus. A coagglutination reagent was constructed by arming S. aureus Cowan I cells with the anti-V. vulnificus flagellar antibody. The reagent coagglutinated 99.3% of isolates identified bacteriologically as V. vulnificus and, other …
Characterization And Application Of Monoclonal Antibody And Bovine Neutrophil Reactivity To Pasteurella Haemolytica Antigens., Frank William Austin
Characterization And Application Of Monoclonal Antibody And Bovine Neutrophil Reactivity To Pasteurella Haemolytica Antigens., Frank William Austin
LSU Historical Dissertations and Theses
Monoclonal antibodies (McAbs) were prepared against Pasteurella haemolytica serotype 1 (Ph-1) capsular material to identify, quantitate and purify antigens. McAbs were selected by ELISA and characterized according to their isotype, cross-reactivity with P. haemolytica and P. multocida serotypes in ELISA, antigen formalin sensitivity and ability to cause bacterial agglutination. Four groups of McAbs were established each recognizing a different epitope. Various P. haemolytica and P. multocida serotypes were found to share several epitopes demonstrating their antigenic relatedness. A colorimetric assay, based on tetrazolium dye reduction by bovine neutrophils, was adapted for the measurement and characterization of Ph-1 cytotoxin activity. Using …
Cell Division Studies Of Escherichia Coli: Expression And Protein Localization Of Cell Septation Gene, Ftsa., Chong Chon Younghae
Cell Division Studies Of Escherichia Coli: Expression And Protein Localization Of Cell Septation Gene, Ftsa., Chong Chon Younghae
LSU Historical Dissertations and Theses
FtsA is a gene essential for cell septation. It has been postulated to have both regulatory and structural functions. To study the structural involvement of the FtsA protein in septum formation, four FtsA-LacZ protein fusions were constructed using a mini-Mu transposon (MudII1734). When maxicells containing either ftsA-lacZ or ftsA specifying plasmids were radioactively labelled and fractionated, FtsA-LacZ fusion protein and FtsA were located in both the membrane and cytoplasmic fractions. The FtsA-LacZ fusion proteins were also located in dividing wild type dividing cells by measuring $\beta$-galactosidase activity. Cell envelope fractions from a sucrose equilibrium gradient had $\beta$-galactosidase activity in outer …
Pseudomonas Sp. Strain Pg2982: Uptake Of Glyphosate And Cloning Of A Gene Which Confers Increased Resistance To Glyphosate., Joseph E. Fitzgibbon
Pseudomonas Sp. Strain Pg2982: Uptake Of Glyphosate And Cloning Of A Gene Which Confers Increased Resistance To Glyphosate., Joseph E. Fitzgibbon
LSU Historical Dissertations and Theses
Chapter 1. Pseudomonas sp. strain PG2982 has the ability to use the phosphonate herbicide, glyphosate, as a sole phosphorus source. Glyphosate uptake occurs at a maximum in the late log phase of growth and is induced by phosphate starvation. Uptake is inhibited by phosphate and arsenate, but not by the amino acids glycine and sarcosine. The K$\sb{\rm m}$ and V$\sb{\rm max}$ for glyphosate uptake were calculated to be 23uM and 0.97nmoles/mg dry wt/min, respectively. A phosphate transport system with a broad substrate specificity seems to be responsible for glyphosate uptake. Chapter 2. Pseudomonas sp. strain PG 2982 is highly resistant …