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2003

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Environmental & Architectural Phenomenology. Vol. 15, No. 1, Kansas State University. Architecture Department Dec 2003

Environmental & Architectural Phenomenology. Vol. 15, No. 1, Kansas State University. Architecture Department

Environmental & Architectural Phenomenology

No abstract provided.

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Efficient High-Volume Cleaning Of Aspergillus Nidulans Cleistothecia Using Bare Fingers., Terry W. Hill, Darlene M. Loprete, Jared A. Castagna, Samuel O. Weems Dec 2003

Efficient High-Volume Cleaning Of Aspergillus Nidulans Cleistothecia Using Bare Fingers., Terry W. Hill, Darlene M. Loprete, Jared A. Castagna, Samuel O. Weems

Fungal Genetics Reports

Even seasoned workers find it tedious and sometimes frustrating to remove Hülle cells and stray conidia from Aspergillus cleistothecia by rolling them with forceps across an agar surface, particularly when large numbers must be cleaned. It can be even more challenging to teach the skill to others, especially to a whole class of easily discouraged undergraduates, who may be seeing their first high-mag image of forceps tips at the same time as their first view of a cleistothecium.

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Split-Marker Recombination For Efficient Targeted Deletion Of Fungal Genes, Natalie L. Catlett, Bee-Na Lee, O. C. Yoder, B. Gillian Turgeon Dec 2003

Split-Marker Recombination For Efficient Targeted Deletion Of Fungal Genes, Natalie L. Catlett, Bee-Na Lee, O. C. Yoder, B. Gillian Turgeon

Fungal Genetics Reports

A commonly used method for fungal gene deletion is introduction of linear DNA consisting of a selectable marker gene flanked on both sides by short stretches of DNA that target a gene of interest (Wirsel et al 1996 Curr. Genet 29:241-249). Gene deletion in Cochliobolus heterostrophus and Gibberella zeae occurs efficiently with this approach. To facilitate deletion construct synthesis, we have applied the "split-marker” deletion strategy previously developed for Saccharomyces cerevisiae (Fairhead et al. 1996 Yeast 12:1439-57; Fairhead et al. 1998 Gene 223:33-46). Here, we describe both fusion PCR-based and plasmid-based deletion methods using this strategy with PEG-mediated protoplast transformation …

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Vogel's Medium N Salts: Avoiding The Need For Ammonium Nitrate, Robert L. Metzenberg Dec 2003

Vogel's Medium N Salts: Avoiding The Need For Ammonium Nitrate, Robert L. Metzenberg

Fungal Genetics Reports

Vogel's Medium N salts (Vogel, 1956 Microbiol. Genet. Bull. 13:42-43), supplemented with a carbon source, is widely used for the cultivation of Neurospora and many other fungi. The standard recipe includes ammonium nitrate.

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Isolation Of Genomic Dna From Acetone-Dried Aspergillus Mycelia, N. S. Punekar, S. V. Suresh Kumar, T. N. Jayashri, R. Anuradha Dec 2003

Isolation Of Genomic Dna From Acetone-Dried Aspergillus Mycelia, N. S. Punekar, S. V. Suresh Kumar, T. N. Jayashri, R. Anuradha

Fungal Genetics Reports

A simple acetone-drying protocol was adopted to replace the lyophilization step while isolating genomic DNA from Aspergillus mycelia. This DNA is suitable for PCR, restriction enzyme digestion and Southern blot analysis with digoxigenin-labeled DNA probes. Acetone drying/ preservation can be a useful method in the molecular analysis of fungal DNA samples.

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Raymond W. Barratt, 1920-2002, Dorothy Newmeyer, David D. Perkins Dec 2003

Raymond W. Barratt, 1920-2002, Dorothy Newmeyer, David D. Perkins

Fungal Genetics Reports

Raymond W. Barratt 1920-2002

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Different Cell Types In Neurospora Crassa, George N. Bistis, David D. Perkins, Nick D. Read Dec 2003

Different Cell Types In Neurospora Crassa, George N. Bistis, David D. Perkins, Nick D. Read

Fungal Genetics Reports

Neurospora possesses more cell types than are commonly recognized. We have been able to identify 28 morphologically distinct types. Having the cell types clearly defined will be important for genome annotation, describing new mutant phenotypes, and determining sites of gene expression.

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Alexandra Putrament, 1926-2003, David D. Perkins Dec 2003

Alexandra Putrament, 1926-2003, David D. Perkins

Fungal Genetics Reports

Alexandra Putrament, 1926-2003

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Wacslaw Gajewski, 1911-1997, David D. Perkins Dec 2003

Wacslaw Gajewski, 1911-1997, David D. Perkins

Fungal Genetics Reports

Wacslaw Gajewski, 1911-1997

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Thad H. Pittenger, 1921-2003, Helmut Bertrand Dec 2003

Thad H. Pittenger, 1921-2003, Helmut Bertrand

Fungal Genetics Reports

Thad H. Pittenger, 1921-2003

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Neurospora Tetrasperma Bibliography - Additions, David D. Perkins Dec 2003

Neurospora Tetrasperma Bibliography - Additions, David D. Perkins

Fungal Genetics Reports

During the evolutionary divergence of N. tetrasperma from the eight-spored Neurospora species, ascus development was reprogrammed with the result that each of the four large ascospores is heterokaryotic, containing nuclei of both mating types, and germlings are self-fertile. Unique features of genome oganization, cell biology, and population structure have attracted investigators to use this pseudohomothallic, four-spored species for a wide range of studies. A bibliography listing 164 publications was published in 1994 in Fungal Genetics Newsletter 41:72-78. Eighty-six additional publications are listed here. In addition to recent papers, these include some theses, abstracts, and papers that were omitted from the …

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Podospora Anserina Bibliography N° 10 - Additions, Robert Debuchy Dec 2003

Podospora Anserina Bibliography N° 10 - Additions, Robert Debuchy

Fungal Genetics Reports

Podospora anserina is a coprophilous fungus growing on herbivore dung. It is a pseudohomothallic species in which ascus development results, as in Neurospora tetrasperma but through a different process, in the formation of four large ascospores containing nuclei of both mating types.

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Neurospora Bibliography, Craig Wilson Dec 2003

Neurospora Bibliography, Craig Wilson

Fungal Genetics Reports

This bibliography represents my attempt to collect all works dealing substantially with Neurospora. Please let me know of anything published in 2002 or 2003 that is not included here or in the previous bibliography, so that it might be mentioned next year. I would be especially happy to hear of chapters from books, and articles from journals not indexed in major bibliographic services. Please also let me know of any errors in citation. Please send reprints or copies of articles to the Fungal Genetics Stock Center.

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Foreword To Fgn 50, Online Edition, Matthew S. Sachs Dec 2003

Foreword To Fgn 50, Online Edition, Matthew S. Sachs

Fungal Genetics Reports

Editorial board, publisher, and contact information.

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A Gateway™ Destination Vector For High-Throughput Construction Of Neurospora Crassa Histidine-3 Gene Replacement Plasmids, Jeremy R. Haag, Dong W. Lee, Rodolfo Aramayo Dec 2003

A Gateway™ Destination Vector For High-Throughput Construction Of Neurospora Crassa Histidine-3 Gene Replacement Plasmids, Jeremy R. Haag, Dong W. Lee, Rodolfo Aramayo

Fungal Genetics Reports

We report the construction of a Destination Vector, called pJHAM007, for the targeted integration of DNA sequences at the histidine-3 (his-3) locus of Neurospora crassa. pJHAM007 has all the necessary features required to perform a simple, rapid and efficient GATEWAY™ recombinational cloning with an Entry Clone to yield a his-3-gene replacement Destination Vector.

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No Evidence For Intra-Allelic Complementation At The Osmotic-1 Locus Of Neurospora Crassa, Claude P. Selitrennikoff, Shelly Wilson, Samantha Renault, Rebecca O’Rourke Dec 2003

No Evidence For Intra-Allelic Complementation At The Osmotic-1 Locus Of Neurospora Crassa, Claude P. Selitrennikoff, Shelly Wilson, Samantha Renault, Rebecca O’Rourke

Fungal Genetics Reports

The Neurospora crassa osmotic-1 locus (os-1) encodes a protein with homology to two component histidine kinase sensors. We formed forced heterokaryons between each of ten os-1 alleles in all pair-wise combinations and found, in contrast to a previous report, no evidence for intra-allelic complementation.

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Aspergillus Bibliography, John Clutterbuck Dec 2003

Aspergillus Bibliography, John Clutterbuck

Fungal Genetics Reports

This bibliography attempts to cover genetical and biochemical publications on Aspergillus nidulans and also includes selected references to related species and topics. Entries have been checked as far as possible, but please tell me of any errors and omissions. Authors are kindly requested to send a copy of each article to the FGSC for its reprint collection.

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Helga Ninnemann, 1938-2003, Josef Maier, Stuart Brody Dec 2003

Helga Ninnemann, 1938-2003, Josef Maier, Stuart Brody

Fungal Genetics Reports

Helga Ninnemann, 1938-2003

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22nd Fungal Genetics Conference At Asilomar, Fungal Genetics Conference Dec 2003

22nd Fungal Genetics Conference At Asilomar, Fungal Genetics Conference

Fungal Genetics Reports

Abstracts from the 2003 Fungal Genetics Conference at Asilomar

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Environmental & Architectural Phenomenology Vol. 14, No. 3, Kansas State University. Architecture Department Sep 2003

Environmental & Architectural Phenomenology Vol. 14, No. 3, Kansas State University. Architecture Department

Environmental & Architectural Phenomenology

No abstract provided.

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Communicating Biotechnology: Relationships Between Tone, Issues, And Terminology In U.S. Print Media Coverage, Jefferson D. Miller, Mamane Annou, Eric J. Wailes Sep 2003

Communicating Biotechnology: Relationships Between Tone, Issues, And Terminology In U.S. Print Media Coverage, Jefferson D. Miller, Mamane Annou, Eric J. Wailes

Journal of Applied Communications

As part of a nationwide study on the public acceptability of agricultural biotechnology, researchers performed a content analysis on two years of print news coverage of biotechnology (2000-2002). Qualitative analysis methods included examining text from selected national newspapers, regional newspapers, and trade publications for common issues, tone, and terminology. A team of three coders, trained to an acceptable level of agreement (Cohen’s K = .80), examined and coded the articles. The relationship between the tone of the article and the terminology used in reference to biotechnology (e.g., “genetically engineered,” “genetically modified,” or “biotechnology”) has practical applications for both public relations …

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How Newspapers In Kansas Are Using News Releases From K-State Research And Extension, Pat Melgares, Tracy Rutherford, Lori Alexander Sep 2003

How Newspapers In Kansas Are Using News Releases From K-State Research And Extension, Pat Melgares, Tracy Rutherford, Lori Alexander

Journal of Applied Communications

This study looks at how newspaper editors in Kansas use news releases from Kansas State University Research and Extension (KSRE), which distributes more than 700 news releases annually to newspapers across the state. An analysis of clippings over 10 weeks showed that editors rarely change the original text or headline in KSRE news releases, and more than half of the releases were published within 10 days of release. KSRE news releases were published in all regions of Kansas. Using a formula to determine the advertising equivalency value of news clips, the data indicates a value of $33,286.94 for the sample …

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Table Of Contents And Editorial Information For Vol. 31, No. 1, Fall 2003, Mary P. Mckeown-Moak Sep 2003

Table Of Contents And Editorial Information For Vol. 31, No. 1, Fall 2003, Mary P. Mckeown-Moak

Educational Considerations

Table of contents and editorial information for Vol. 31, no. 1, Fall 2003

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Foreword, Mary P. Mckeown-Moak Sep 2003

Foreword, Mary P. Mckeown-Moak

Educational Considerations

This edition of Educational Considerations is devoted to the issues impacting on higher education at the beginning of the 21st century: declining revenues from all sources; increasing demands for accountability; increasing costs leading to increases in tuition and fees; enrollment management; and the changing role of state governments.

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Enrollment Management And Tuition Discounting, Lucie Lapovsky, Loren Loomis Hubbell Sep 2003

Enrollment Management And Tuition Discounting, Lucie Lapovsky, Loren Loomis Hubbell

Educational Considerations

Enrollment management is critical to the success of all colleges and universities, and especially in times when state appropriations are not increasing, the endowment is not producing at historical levels, and gifts are not keeping pace with inflation.

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The Changing Financial And Policy Role Of State Governments Regarding Higher Education And Prospects For The Future, Daniel T. Layzell Sep 2003

The Changing Financial And Policy Role Of State Governments Regarding Higher Education And Prospects For The Future, Daniel T. Layzell

Educational Considerations

Higher education in the United States grew rapidly in the post-World War II era, more than doubling the total number of degree-granting institutions (1,851 to 4,084) and increasing the total number of students enrolled more than five-fold (2.7 million to 14.8 million) between 1950 and 2000.

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Performance Reporting: The Preferred "No Cost" Accountability Program, Joseph C. Burke, Henrik P. Minassians Sep 2003

Performance Reporting: The Preferred "No Cost" Accountability Program, Joseph C. Burke, Henrik P. Minassians

Educational Considerations

For the last six years, the Rockefeller Institute of Government at the State University of New York at Albany has been surveying the State Higher Education Finance Officers (hereafter referred to as budget officers) regarding state activities in higher education performance funding and budgeting.

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Educational Considerations, Vol. 31(1) Full Issue, Mary P. Mckeown-Moak Sep 2003

Educational Considerations, Vol. 31(1) Full Issue, Mary P. Mckeown-Moak

Educational Considerations

Educational Considerations, vol. 31(1) Fall 2003 - Full issue

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Public Education's Perfect Storm, J. Kent Caruthers Sep 2003

Public Education's Perfect Storm, J. Kent Caruthers

Educational Considerations

Today, a confluence of six sets of trends are serving to create what is likely to become regarded as the “perfect storm” for funding of public higher education.

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Financing Higher Education In Fiscal Year 2003: The State Of The States, Mary P. Mckeown-Moak Sep 2003

Financing Higher Education In Fiscal Year 2003: The State Of The States, Mary P. Mckeown-Moak

Educational Considerations

So begins the 2002 Fiscal Survey of the States from the National Governors Association. Nearly every state has reduced budgets and cut expenditures in light of reductions in anticipated state revenues.

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