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Fungal Genetics Newsletter #53 - 2006, Matthew S. Sachs
Fungal Genetics Newsletter #53 - 2006, Matthew S. Sachs
Fungal Genetics Reports
Editorial board, publisher, and contact information.
Application Of The Nourseothricin Acetyltransferase Gene (Nat1) As Dominant Marker For The Transformation Of Filamentous Fungi, Ulrich Kück, Birgit Hoff
Application Of The Nourseothricin Acetyltransferase Gene (Nat1) As Dominant Marker For The Transformation Of Filamentous Fungi, Ulrich Kück, Birgit Hoff
Fungal Genetics Reports
Here, we report the construction of two transformation vectors, pD-NAT1 and pG-NAT1, carrying the nat1 gene encoding the nourseothricin acetyltransferase. The nat1 gene is expressed under the control of the Aspergillus nidulans trpC promoter and thus can be used as a dominant drug-resistance marker for the DNA-mediated transformation of filamentous fungi. The successful application of both vectors was demonstrated by transforming the homothallic ascomycete Sordaria macrospora as well as the β-lactam producerAcremonium chrysogenum. For both fungi and for both vectors, transformation frequencies were between 10 and 40 transformants per 10 µg of plasmid DNA.
Neurospora Msh4 Ortholog Confirmed By Split-Marker Deletion, Sue Conway, Fred Bowring, Jane Yeadon, David Catcheside
Neurospora Msh4 Ortholog Confirmed By Split-Marker Deletion, Sue Conway, Fred Bowring, Jane Yeadon, David Catcheside
Fungal Genetics Reports
Although most eukaryotes have both MSH4 and MSH5 orthologs, Neurospora was initially thought to lack msh-4. We have deleted the most likely msh-4 candidate and observed a delay in the sexual cycle, disruption to meiosis and a reduction in fertility. Deletion is dominant, showing msh-4 is subject to MSUD. We conclude that Neurospora has a MSH4 ortholog and that it may have remained undetected because of an unusually high number of introns.
Glycosyl Hydrolase Genes And Enzymes Of Neurospora Crassa, Alan Radford
Glycosyl Hydrolase Genes And Enzymes Of Neurospora Crassa, Alan Radford
Fungal Genetics Reports
An analysis of the genome of Neurospora crassa has identified genes encoding 84 putative glycosyl hydrolases, representing 24 different families in Henrissat's classification. Functionally, they include enzymes for the degradation of all major polysaccharides (including cellulase, hemicellulase, chitinase and pectinase).There is evidence of high levels of gene amplification, despite the presence of RIP, as there are eight representatives of family 3 (beta-glucosidases and xylosidases), five of family 7 (endo- and exo-glucanases), six of family 13 (amylases and maltase), nine of family 18 (chitinase), eight of family 47 (ER alpha-mannosidases), eleven of family 61 (endoglucanases) and seven of family 76 (alpha-mannanases).
Sequences Important For Heterokaryon Incompatibility Function In Mat A-1 Of Neurospora Crassa, Patrick K.T. Shiu, N. Louise Glass
Sequences Important For Heterokaryon Incompatibility Function In Mat A-1 Of Neurospora Crassa, Patrick K.T. Shiu, N. Louise Glass
Fungal Genetics Reports
Using chimeric constructs between the Neurospora crassa mat A-1 gene and the Podospora anserina FMR1 gene, we identified the amino acids important for the heterokaryon incompatibility function in the mating-type protein MAT A-1.
Non-Circadian Light Inducible Rhythm In Aspergillus Nidulans, Sijmen E. Schoustra, Marijke Slakhorst, Rolf F. Hoekstra, Alfons J.M. Debets
Non-Circadian Light Inducible Rhythm In Aspergillus Nidulans, Sijmen E. Schoustra, Marijke Slakhorst, Rolf F. Hoekstra, Alfons J.M. Debets
Fungal Genetics Reports
Aspergillus nidulans grown under standard laboratory conditions does not show circadian rhythmic growth. The presence of a circadian clock was demonstrated in A. nidulans at the level of gene expression (Greene et al. 2003), but a visually observable rhythm is lacking. We recently observed a remarkable rhythmic growth pattern in the formation of conidiospores and ascospores in a fludioxonil resistant mutant of A. nidulans (fldA1) grown in a dark incubator. This is reminiscent of a circadian rhythm. We found however that our observed rhythm is induced by light (leaking into the ‘dark’ incubator) and is not self-sustainable. In absolute darkness …
Influence Of Lithium Ions On Conidiophore Size In Neurospora Crassa, Bodil Aase, Ingunn W. Jolma, Peter Ruoff
Influence Of Lithium Ions On Conidiophore Size In Neurospora Crassa, Bodil Aase, Ingunn W. Jolma, Peter Ruoff
Fungal Genetics Reports
Lithium (Li) ions are known to affect Neurospora crassa’s growth speed and circadian clock period, while elevated temperatures abolish these influences. We wondered whether Li has also an effect on conidia size. We used cryo-SEM to investigate this question and report here the results of 1720 measurements showing that at 20°C the long and short conidial axes are significantly reduced at high Li concentrations (10-15 mM), while the ratio between the long and short axes remains approximately constant. An increased temperature (30°C) appears to abolish the Li effect on conidia size.
The Effect Of Repeated Freeze-Thaw Cycles On Cryopreserved Neurospora Crassa Samples., Kevin Mccluskey, Aric Wiest, Sheera Walker
The Effect Of Repeated Freeze-Thaw Cycles On Cryopreserved Neurospora Crassa Samples., Kevin Mccluskey, Aric Wiest, Sheera Walker
Fungal Genetics Reports
To better characterize handling parameters for the arrayed mutants prepared for the Neurospora functional genomics program, we have put 7 day old conidia from strain FGSC 2489 through a series of cycles of freezing at -80 C in 25% glycerol and 3.5% reconstituted non-fat dry milk.
A Simple Way To Make A Dilution Series, Robert L. Metzenberg
A Simple Way To Make A Dilution Series, Robert L. Metzenberg
Fungal Genetics Reports
Often one needs to determine a suitable concentration of a previously untested nutrient or inhibitor to use in subsequent experiments.
A Ras-1Bd Mauriceville Strain For Mapping Mutations In Oak Ridge Ras-1BdStrains, A. Kaitlyn Beasley, Teresa M. Lamb, Wayne K. Versaw, Deborah Bell-Pedersen
A Ras-1Bd Mauriceville Strain For Mapping Mutations In Oak Ridge Ras-1BdStrains, A. Kaitlyn Beasley, Teresa M. Lamb, Wayne K. Versaw, Deborah Bell-Pedersen
Fungal Genetics Reports
We describe the construction of a Neurospora crassa Mauriceville strain carrying the ras-1bd mutation marked by the bacterial hygromycin resistance gene, hph (new FGSC # 10156). This strain is valuable for mapping mutations in Oak Ridge strains that carry the bd mutation.
Abstracts From The Neurospora 2006 Conference, Neurospora Conference
Abstracts From The Neurospora 2006 Conference, Neurospora Conference
Fungal Genetics Reports
Plenary and poster session abstracts from the Neurospora 2006 Conference
Fungal Genetics Stock Center Catalogue Of Strains, Kevin Mccluskey, M. Plamann
Fungal Genetics Stock Center Catalogue Of Strains, Kevin Mccluskey, M. Plamann
Fungal Genetics Reports
Catalogue of Strains, 11th edition, 2006, supplement to Fungal Genetics Newsletter No. 53. This catalogue contains lists of materials held by the Fungal Genetics Stock Center.
High Throughput Mating Tests In Neurospora Crassa, Kevin Mccluskey, Rachel L. Yedlin, Sheera A. Walker
High Throughput Mating Tests In Neurospora Crassa, Kevin Mccluskey, Rachel L. Yedlin, Sheera A. Walker
Fungal Genetics Reports
Mating type tests in Neurospora crassa can be laborious and tedious. Since we were arraying mutants for distribution, we have developed a method to carry out mating type tests in 96-well plates. This technique is highly efficient and reproducible and gives results in three to four days.
Podospora Anserina As6 Gene Encodes The Cytosolic Ribosomal Protein Of The E. Coli S12 Family, Michelle Dequard-Chablat, Philippe Silar
Podospora Anserina As6 Gene Encodes The Cytosolic Ribosomal Protein Of The E. Coli S12 Family, Michelle Dequard-Chablat, Philippe Silar
Fungal Genetics Reports
The ribosomal proteins of the E. coli S4, S5 and S12 families that are part of the ribosome accuracy center control translation accuracy both in prokaryotes and eukaryotes. In Podospora anserina, genes coding for S4 and S5 have already been identified. Here, we identify the gene coding for the S12 protein homologue and show that it is identical to the genetically known AS6 gene.
Aspergillus Bibliography 2006, John Clutterbuck
Aspergillus Bibliography 2006, John Clutterbuck
Fungal Genetics Reports
This bibliography attempts to cover genetical and biochemical publications on Aspergillus nidulans and also includes selected references to related species and topics. Entries have been checked as far as possible, but please tell me of any errors and omissions. Authors are kindly requested to send a copy of each article to the FGSC for its reprint collection.
Neurospora Bibliography, Craig H. Wilson
Neurospora Bibliography, Craig H. Wilson
Fungal Genetics Reports
This bibliography represents my attempt to collect all works dealing substantially with Neurospora. Please let me know of anything published in 2005 or 2006 that is not included here or in the previous bibliography, so that it might be mentioned next year. I would be especially happy to hear of chapters from books, and articles from journals not indexed in major bibliographic services. Please also let me know of any errors in citation. Please send reprints or copies of articles to the Fungal Genetics Stock Center.