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Iowa State University

Mark R. Ackermann

1988

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Acute Fibrinopurulent Blepharitis And Conjunctivitis Associated With Staphylococcus Hyicus, Escherichia Coli, And Streptococcus Sp. In Chickens And Turkeys, N. F. Cheville, J. Tappe, Mark R. Ackermann, A. Jensen Sep 1988

Acute Fibrinopurulent Blepharitis And Conjunctivitis Associated With Staphylococcus Hyicus, Escherichia Coli, And Streptococcus Sp. In Chickens And Turkeys, N. F. Cheville, J. Tappe, Mark R. Ackermann, A. Jensen

Mark R. Ackermann

Multiple outbreaks of acute severe fibrinopurulent lesions of the eyelids occurred in chickens and turkeys. Lesions began as tiny foci of epidermal necrosis and ulceration and spread to involve the entire eyelid. Scabs overlying the epidermis contained large gram-positive cocci; lesser numbers of small cocci and gram-negative bacilli were in more superficial areas. Staphylococcus hyicus was isolated from birds in all stages of the disease. Escherichia coli and Streptococcus sp. were isolated only during severe stages; no anaerobic bacterial pathogens were isolated. Vasculitis and perivascular lymphocytic infiltrates in deep layers of the dermis suggested that a staphylococcal toxin may have …


Bovine Ileal Dome Lymphoepithelial Cells: Endocytosis And Transport Of Brucella Abortus Strain 19, Mark R. Ackermann, N. F. Cheville, B. L. Deyoe Jan 1988

Bovine Ileal Dome Lymphoepithelial Cells: Endocytosis And Transport Of Brucella Abortus Strain 19, Mark R. Ackermann, N. F. Cheville, B. L. Deyoe

Mark R. Ackermann

Ligated ileal loops of calves were inoculated with Brucella abortus and examined at 2, 4, 6, 10, and 24 hours post-inoculation. B. abortus was identified by light and electron microscopy using immunoperoxidase and antibody-coated colloidal gold techniques. B. abortus was detected in vesicles, phagolysosomes, and large vacuoles of lymphoepithelial cells. Numbers of intracellular bacteria decreased with time after inoculation. B. abortus was also seen between and below lymphoepithelial cells and free in the dome interstitium and intestinal lymph vessels. Neutrophils and macrophages in both epithelium and lamina propria contained intact or degraded bacteria within phagosomes, phagolysosomes, and multivesicular bodies. These …