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Isolation Of A Somatic Cell Mutant Resistant To The Induction Of Apoptosis By Oxidized Low Density Lipoprotein, Antonio E. Rusiñol, Lin Yang, Douglas Thewke, Sankhavaram R. Panini, Marianne F. Kramer, Michael S. Sinensky
Isolation Of A Somatic Cell Mutant Resistant To The Induction Of Apoptosis By Oxidized Low Density Lipoprotein, Antonio E. Rusiñol, Lin Yang, Douglas Thewke, Sankhavaram R. Panini, Marianne F. Kramer, Michael S. Sinensky
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Oxidized low density lipoprotein (oxLDL) induces apoptosis in macrophages, smooth muscle cells, and endothelial cells. To elucidate the molecular mechanism of oxLDL-induced cytotoxicity and determine its tissue specificity, we have used Chinese hamster ovary (CHO)-K1 cells expressing human CD36 (CHO/CD36). Expression of CD36 rendered these cells susceptible to killing by oxLDL. This cytotoxicity was due to the induction of apoptosis. Therefore, CD36 expression is the only requirement for oxLDL-induced apoptosis. Oxysterols apparently mediate the cytotoxicity of oxLDL in macrophage foam cells and endothelial cells. 25-Hydroxycholesterol, at concentrations higher than 1 μg/ml, killed CHO-K1 cells, by apoptosis, in medium supplemented with …