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Tyrosine Phosphorylation Of C-Erbb-2 Is Regulated By The Cellular Form Of Prostatic Acid Phosphatase In Human Prostate Cancer Cells, Tzu Ching Meng, Ming Fong Lin
Tyrosine Phosphorylation Of C-Erbb-2 Is Regulated By The Cellular Form Of Prostatic Acid Phosphatase In Human Prostate Cancer Cells, Tzu Ching Meng, Ming Fong Lin
ETSU Faculty Works
Human prostatic acid phosphatase (PAcP) is a prostate epithelium- specific differentiation antigen. In prostate carcinomas, the cellular PAcP is decreased. We investigated its functional role in these cells. Several lines of evidence support the hypothesis that cellular PAcP functions as a neutral protein-tyrosine phosphatase and is involved in regulating prostate cell growth. In this study, we identify its in vivo substrate. Our results demonstrated that, in different human prostate cancer cell lines, the phosphotyrosine (Tyr(P)) level of a 185-kDa phosphoprotein (pp185) inversely correlates with the cellular activity of PAcP. On SDS-PAGE, this pp185 comigrates with the c-ErbB-2 oncoprotein. Immunodepletion experiments …
Oleate Potentiates Oxysterol Inhibition Of Transcription From Sterol Regulatory Element-1-Regulated Promoters And Maturation Of Sterol Regulatory Element-Binding Proteins, Douglas P. Thewke, Sankhavaram R. Panini, Michael Sinensky
Oleate Potentiates Oxysterol Inhibition Of Transcription From Sterol Regulatory Element-1-Regulated Promoters And Maturation Of Sterol Regulatory Element-Binding Proteins, Douglas P. Thewke, Sankhavaram R. Panini, Michael Sinensky
ETSU Faculty Works
Activation of genes containing SRE-1 (sterol regulatory element 1) sequences is known to be under the regulation of sterols through modulation of the proteolytic maturation of SREBPs (SRE-1-binding proteins). Previous work has demonstrated SREBP-mediated transcriptional activation of genes encoding enzymes of sterol and fatty acid biosynthesis. Because synthesis of both sterols and C18 fatty acids are required for cell growth, in the absence of exogenous supplements of these lipids, we examined the hypothesis that fatty acid can also be regulatory in SREBP maturation. Our data indicate that C18 fatty acids can potentiate the biological activities of a typical, regulatory sterol: …