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Butler University

Theses/Dissertations

2014

Mnium cuspidatum

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Role Of 12-Oxo-Phytodienoic Acid And Β-Glucan Elicitor In Prevention Of Fungal Infection In Mnium Cuspidatum, Robert E. Spiller May 2014

Role Of 12-Oxo-Phytodienoic Acid And Β-Glucan Elicitor In Prevention Of Fungal Infection In Mnium Cuspidatum, Robert E. Spiller

Undergraduate Honors Thesis Collection

The moss, Mnium cuspidatum and the fungus, Pythium irregulare, were used to study the role of the plant hormone, 12-oxo-phytodienoic acid (OPDA) and the elicitor, β-glucan in the prevention of fungal infection. Each compound was applied before the inoculation of a fungal cube and a combination of qualitative and quantitative analyses were done in order to determine the degree of infection within the moss. It was found that P. irregulare does infect and kill the cells of M. cuspidatum through chloroplast degradation, stem browning and appressoria injections within 24 hours. The pre-application of OPDA or β-glucan were unable to prevent …


Characterization Of Putative Defense Genes In Nonvascular Plants, Blaine Harlan Jan 2014

Characterization Of Putative Defense Genes In Nonvascular Plants, Blaine Harlan

Undergraduate Honors Thesis Collection

Vascular plants have many known defenses against herbivory and pathogen infection. One inducible defense system that has been extensively studied in vascular plants is systemic acquired resistance (SAR), which is a plant-wide response that results in resistance to a wide range of pathogens Many genes that play a role in SAR have been characterized. Although several studies of plant-pathogen interactions in non-vascular plants have occurred, it was not until recently that the existence of SAR was shown in these plants. The goal of the present study was to confirm the presence of homologous defense genes in moss, and to study …


The Influence Of Light On Bryophytes And Their Response To Pathogen Infection: A Story Of Mnium Cuspidatum And Physcomitrella Patens, Bryce James Fawcett Jan 2014

The Influence Of Light On Bryophytes And Their Response To Pathogen Infection: A Story Of Mnium Cuspidatum And Physcomitrella Patens, Bryce James Fawcett

Undergraduate Honors Thesis Collection

The influence of light on non-vascular plant-pathogen interactions were studied in two moss species, Mnium cuspidatum and Physcomitrella patens, using a fungal pathogen, Pythium irregulare, and a commonly used pathogenic elicitor (beta-glucan). The findings of this study suggest that light does playa role in moss defense but speci fic mechanisms remain unclear. In P. patens it was found that the defense gene AOe was up-regulated in the light compared to the dark when treated with beta-glucan elicitor. A phenotypic study of M. cuspidatum revealed that P. irregulare will infect and M cuspidatum will subsequently mount a defense response. Unexpectedly, samples …