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Physical Basis Of The Inducer-Dependent Cooperativity Of The Central Glycolytic Genes Repressor/Dna Complex, Denis Chaix, Matthew L. Ferguson, Cedric Atmanene, Alain Van Dorsselaer, Sarah Sanglier-Cianférani, Catherine A. Royer, Nathalie Declerck

Matthew L. Ferguson

The Central glycolytic genes Repressor (CggR) from Bacillus subtilis belongs to the SorC family of transcription factors that control major carbohydrate metabolic pathways. Recent studies have shown that CggR binds as a tetramer to its tandem operator DNA sequences and that the inducer metabolite, fructose 1,6-bisphosphate (FBP), reduces the binding cooperativity of the CggR/DNA complex. Here, we have determined the effect of FBP on the size, shape and stoichiometry of CggR complexes with full-length and half-site operator sequence by small-angle X-ray scattering, size-exclusion chromatography, fluorescence cross-correlation spectroscopy and noncovalent mass spectrometry (MS). Our results show that CggR forms a compact …