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In Vitro Morphogenesis And Mutation Of Glycine Max L., Thomas Daniel Wilson
In Vitro Morphogenesis And Mutation Of Glycine Max L., Thomas Daniel Wilson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Soybean (Glycine max L. c.v. Consoy) tissue cultures were grown in synthetic media containing various concentrations and combinations of minerals, auxins, cytokinins, pH levels, and inorganic growth factors in a systematic procedure to establish optimum media for initiation and maintenance of callus. Further modification of the hormone level was employed to initiate callus morphogenesis. Normally toxic levels of atrazine were established in soybean calli media, and ethyl methanesulfonate (EMS) and X ray-induced mutant calli were selected for atrazine tolerance.
Optimal media for initiation and maintenance of soybean calli were developed and a defined medium was determined that consistently yields …