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Characterization Of De Novo Fatty Acid Biosynthesis In Soybean Somatic Embryo Plastids, Karen Clark
Characterization Of De Novo Fatty Acid Biosynthesis In Soybean Somatic Embryo Plastids, Karen Clark
All Theses
A method for the isolation of intact physiologically active plastids from rapidly developing soybean (Glycine max L.) somatic embryos has been developed for the in vitro study of lipid metabolism. Using de novo fatty acid biosynthesis from 14C-acetate as a marker for physiological functionality, the greatest rates of fatty acid biosynthesis were recovered in 3000 x g fractions that were isolated in the presence of 0.5 M sorbitol, with essentially no activity occurring in the 3000 x g supernatant. Plastids purified on 10% Percoll were approximately 70 and 97 % free from mitochondrial and ER contamination, respectively, as judged …