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Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot
Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot
Department of Agronomy and Horticulture: Faculty Publications
Large DNA insert libraries in binary T-DNA vectors can assist in the isolation of the gene(s) under-lying a quantitative trait locus (QTL). Binary vectors facilitate the transfer of large-insert DNA fragments containing a QTL from E. coli to Agrobacterium sp. and then to plants. We constructed two soybean large-insert libraries from cv. Forrest in the pCLD04541 (V41) binary vector after partial digestion of genomic high-molecular-weight DNA with BamHI or HindIII. The libraries contain 76,800 clones with an average insert size of 125 kb, and therefore represent 9.5-fold haploid genome equivalents. Colony hybridization using a chloroplast-specific probe infers that …
Nf00-427 Management Of Seed And Seedling Diseases Of Corn, Jim Stack
Nf00-427 Management Of Seed And Seedling Diseases Of Corn, Jim Stack
University of Nebraska-Lincoln Extension: Historical Materials
This NebFact discusses how to manage seed and seedling diseases of corn.
Ec00-802 Soybean Basis Patterns From Selected Sites In Nebraska, Lynn Lutgen
Ec00-802 Soybean Basis Patterns From Selected Sites In Nebraska, Lynn Lutgen
University of Nebraska-Lincoln Extension: Historical Materials
The following publication contains soybean basis patterns for several towns in Nebraska. The basic price information was collected through surveys, newspaper, electronic media, etc. The listing includes towns that are representative of different geographic locations in Nebraska. The amount of data varies among locations. This publication will be updated each year by adding a year's data to each location which will allow the user to observe the changes in the basis patterns over time.
G00-1395 Soybean Seeding Rates, Roger Wesley Elmore, James E. Specht
G00-1395 Soybean Seeding Rates, Roger Wesley Elmore, James E. Specht
University of Nebraska-Lincoln Extension: Historical Materials
The soybean stand looked horrible late that May. The field had a good seed bed at planting, but rain after planting crusted the soil over the seed furrows. Rain was in the short-term forecast. All other row crops were planted and looked fine, but this field concerned the producer. Should the producer replant? Recommended soybean seeding rates, stand assessment and replanting rates based on Nebraska field research are addressed in this NebGuide.