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The Influence Of Commercial Salmonella Vaccination Programs On Local- And Systemic-Immune Responses And Ceca Microbiome In Egg-Type Chickens, Chrysta Noelle Beck

Graduate Theses and Dissertations

Salmonella vaccination is a widely accepted practice in the United States poultry industry. These vaccines stimulate a strong humoral response to Salmonella serovars and significantly reduce Salmonella load in the bird. In general, a Salmonella vaccination program for an egg-type pullet flock consists of two administrations of a live attenuated Salmonella vaccine at 1- and 6-wk-of-age followed by one administration of a Salmonella bacterin at 12-wk-of-age. Although these vaccines are regarded as effective against paratyphoid Salmonella infections, there are incidences of breakthrough Salmonella infections and severe injection site reactions after bacterin administration. Therefore, the main objective of this dissertation was …

The Design, Construction, And Testing Of Nucleic Acid Vaccines Against Salmonella Enterica Subspecies Enterica Serovar Javiana, Alexis A. Nguyen

LSU Master's Theses

Salmonella spp. is a Gram-negative bacterium that has the ability to replicate within a variety of hosts, or within the environment, including water, soil, and vegetation. These characteristics, coupled with the zoonotic nature of non-typhoidal Salmonella, pose a significant public health concern. Salmonella is the leading cause of foodborne illnesses worldwide, and in the United States alone, the economic impact due to non-typhoidal Salmonella illnesses in 2013 was estimated to be $3.7 billion, the majority of which were due to mortalities. In a time of rising antibiotic resistance, antibiotics are no longer considered the most effective treatment for …

Antibiogram Profiles Of Pathogenic And Commensal Bacteria In Goat And Sheep Feces On Smallholder Farm, Ashesh Basnet, Agnes Kilonzo-Nthenge

Agricultural and Environmental Sciences Faculty Research

Introduction: The increase of antimicrobial resistance (AMR) in zoonotic pathogens poses a substantial threat to both animal production and human health. Although large-scale animal farms are acknowledged as major reservoirs for AMR, there is a notable knowledge gap concerning AMR in small-scale farms. This study seeks to address this gap by collecting and analyzing 137 fecal samples from goat and sheep farms in Tennessee and Georgia.

Method: Bacteria were identified using culture-dependent methods and polymerase chain reaction (PCR), and antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer Disk Diffusion method.

Results and discussion: The prevalence of E. coli (94.9%) …

Gaseous Ozone To Improve The Microbial Safety Of Spices, Seeds, And Nuts, Arshpreet Kaur Khattra

Graduate Theses and Dissertations

Due to the increasing number of reported outbreaks of pathogens in low-moisture foods (LMFs), there is a need to investigate novel non-thermal gaseous technologies to increase the microbiological safety of spices, nuts, and seeds without compromising the product quality. The objective of this study was to inactivate Salmonella spp. in different spices, seeds, and nuts using gaseous ozone treatment and evaluate the suitability of Enterococcus faecium as a potential surrogate for Salmonella. Each food product (dried basil leaves, black peppercorn, chia seeds, and walnuts) was separately inoculated with a 5-strain Salmonella and E. faecium cocktail and equilibrated to water activity …

A Comparison Of The Prevalence Of Salmonella, Specifically S. Enteritidis, In Captive Born Versus Wild Caught Reptiles, Jessica Thomas, Carrie M. Doyle

Undergraduate Theses

Salmonella contamination and transmission is a concern in public health in the United States and around the world. It is usually transmitted to humans through contaminated poultry products, but it has also been known to be transmitted through contact with reptiles. Because reptiles are becoming more popular as pets and are known carriers for Salmonella, studies have been done to test how prevalent Salmonella is in reptiles. This study focused on testing the feces of twenty-two reptiles for Salmonella, in general, and a specific serotype of Salmonella, S. Enteritidis. This study also compared the positivity rate of reptiles born …

Nuclear Factor Kappa B-Dependent Persistence Of, Taylor A Stepien, Larissa A Singletary, Fermin E Guerra, Joyce E Karlinsey, Stephen J Libby, Sarah L Jaslow, Margaret R Gaggioli, Kyle D Gibbs, Dennis C Ko, Michael A Brehm, Dale L Greiner, Leonard D. Shultz, Ferric C Fang

Faculty Research 2024

Salmonella serovars Typhi and Paratyphi cause a prolonged illness known as enteric fever, whereas other serovars cause acute gastroenteritis. Mechanisms responsi ble for the divergent clinical manifestations of nontyphoidal and enteric fever Salmonella infections have remained elusive. Here, we show that S. Typhi and S. Paratyphi A can persist within human macrophages, whereas S. Typhimurium rapidly induces apoptotic macrophage cell death that is dependent on Salmonella pathogenicity island 2 (SPI2). S. Typhi and S. Paratyphi A lack 12 specific SPI2 effectors with pro-apoptotic functions, including nine that target nuclear factor κB (NF-κB). Pharmacologic inhibition of NF-κB or heterologous expression of …

Salmonella In The Environment: A Review On Ecology, Antimicrobial Resistance, Seafood Contaminations, And Human Health Implications, Mohammad Maruf Billah, Md Saydur Rahman

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

Salmonella is a genus of Gram-negative bacteria that is responsible for numerous food poisoning outbreaks worldwide. With 93.8 million food-borne illnesses and 155,000 fatalities annually, it has emerged as a significant global public health issue. There are currently more than 2,500 distinct Salmonella serotypes, and more than half of them are associated with Salmonella enterica. An increasing global public health concern for humans and animals is antimicrobial resistance by Salmonella species worldwide. Salmonella infections can be lethal; conditioned with an increased prevalence of multi-drug resistant (MDR) strains in the future. The emergence of MDR Salmonella serotypes is considerably …

Genome-Based Pathogenicity Potential Of Salmonella Isolated From Diverse Sources, Jared Mr Crocco

Theses and Dissertations (Comprehensive)

Bacterial human pathogens are among the leading causes of death around the world, especially in low income and developing countries. One important element in a bacterium’s ability to cause disease are genes that directly contribute to pathogenicity called virulence factors. A second significant aspect are antimicrobial resistance genes which allow microorganisms to persist in the presence of antimicrobial agents. In this project I aimed to determine if Salmonella isolated from different sources differed in pathogenicity profiles based on the complement of genes identified through genomic analysis. Accordingly, Salmonella genomes were organized into 8 groups: animal, clinical, human, environmental, food, water …

Growth Of Food-Borne Pathogens Listeria And Salmonella And Spore-Forming Paenibacillus And Bacillus In Commercial Plant-Based Milk Alternatives, Klaudia Bartula

ORBioM (Open Research BioSciences Meeting)

An increase in vegan diet preference, lactose intolerance, calorie concern and environmental awareness has led to a rise in the popularity of plant-based alternatives to bovine milk. However, there are still gaps in understanding how known bacterial food contaminants behave in plant-based beverages. The present study is the first to compare the growth of food-pathogens Listeria monocytogenes and Salmonella enterica, food spoilage Bacillus subtilis and an industrial milk product isolate, spore-forming Paenibacillus in commercially available ultrahigh temperature processed bovine milk and plant-based milk alternatives (coconut, almond, cashew). Beverage samples were inoculated with a strain cocktail or individual strains of either …

Rare Case Of Prosthetic Joint Infection Caused By Salmonella Enterica - A Case Report, Carlos Ramos, Shyama Appareddy, Peter J. Gonzalez

Research Colloquium

Introduction: Collectively, over 1 million total knee arthroplasties (TKA) and total hip arthroplasties (THA) are performed in the United States each year. In 2010 it was estimated that 1.58% of the United States general population has received a TKA. Prosthetic joint infections (PJI) are the most common indication for a TKA revision1 . In total, S. aureus is responsible for about 50% of all prosthetic joint infections, and about 15% are caused by gram negative pathogens2 .

Objective: This is a case report which describes the presentation and clinical course of a 59-yearold female with Salmonella enterica septic arthritis of …

Investigation Of The Protein Components In A Periplasmic Mechanism Regulating Bacterial Morphology, Alexandra Pulliam

Electronic Theses and Dissertations

Salmonella is a leading bacterial cause of foodborne illness worldwide. During a previous study investigating the enzymes responsible for regulating cyclic-di-GMP concentrations, a mutant in the cyclic-di-GMP-specific phosphodiesterase STM3615 was identified that displayed a phenotype characterized by decreased survival on agar plates and a shorter bacterium length. I was able to determine that the periplasmic domain of STM3615 was responsible for this phenotype, not the enzymatic phosphodiesterase domain. Based upon a bioinformatic analysis of the protein, I then hypothesized that the periplasmic domain of STM3615 was interacting with a periplasmic protein to give rise to this phenotype. To identify this …

Use Of Transposon Screening For Salicylic Acid-Assisted Desiccation Killing In Salmonella, Shannon D. Elliott

Electronic Theses and Dissertations

Salmonella enterica serovar Typhimurium is one of the most prevalent food-borne pathogens, affecting millions around the world every year, making it a threat to global health. Salmonella possesses the ability to survive the normally lethal condition of desiccation, however, discovery of the genes and mechanisms behind this phenomenon are still ongoing. Using a transposon mutagenesis approach to construct a broad transposon library, this study aimed to uncover genes that may be contributing to changes in Salmonella’s survivability under desiccation, particularly when exposed to the antimicrobial molecule salicylic acid. Building on previous findings showing salicylic acid can alter cell viability …

Radiofrequency And Gaseous Technologies For Enhancing The Microbiological Safety Of Low Moisture Food Ingredients, Surabhi Wason

Graduate Theses and Dissertations

High heat resistance and long survival of Salmonella in low moisture food ingredients (LMFIs) such as spices and seeds are concerning as they are typically consumed without cooking. Therefore, it is challenging to effectively inactivate pathogenic bacteria without negatively impacting the quality of the treated product. This dissertation aimed to develop and evaluate novel intervention technologies: in-package radiofrequency steaming and non-thermal gaseous technologies to improve the microbial safety of LMFIs. The dissertation can be divided into three parts. The first part of this dissertation on the thermal inactivation kinetics of Salmonella and a surrogate, Enterococcus faecium NRRL B-2354on black pepper …

Evaluating Salmonella Cross Contamination In Raw Chicken Thighs In Simulated Post-Chill Tanks, Raziya Sadat

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Salmonella contamination poses a significant risk to food safety, particularly in poultry products and public health. This study aims to investigate the effectiveness of peracetic acid (PAA) treatment in mitigating Salmonella cross-contamination in chicken thighs within simulated post-chill tanks, considering different inoculum concentrations and organic matter (OM) levels.

The experiment involved the use of five distinct Salmonella serovars to inoculate chicken thighs, which were categorized into two groups: higher inoculation level (ca. 7 log CFU/mL) and lower inoculation level (ca, 4 log CFU/mL). These groups underwent treatment with four different solutions, including 540 ppm PAA with 1.5% OM, 540 ppm …

Design, Construction, And Testing Of Recombinant Dna-Based Vaccines For Protection Against Salmonella Enterica Subspecies Enterica Serovar Javiana, Ashley Edwards

LSU Doctoral Dissertations

In the United States, Salmonella Javiana is among the top 5 most common nontyphoidal Salmonella serotypes, with a 325% increase in infection rate since 1970. Globally, nontyphoidal Salmonella leads to approximately 93.8 million illnesses and 155,000 deaths annually, with disproportionate impacts in developing countries. In the United States alone, these infections lead to over $4 billion in costs, the majority of which are attributed to those expenses incurred from mortalities. The zoonotic nature of nontyphoidal Salmonellae and their ability to survive on and within vegetation mean huge economic burden potential for various agricultural industries as well, especially since some of …

Microbiological Safety And Quality Of Ceviche, Poke, And Sushi Dishes Sold At Retail Outlets In Orange County, Ca, Grace E. Marquis, Samantha M. Covaia, Amanda M. Tabb, Courtney J. Kitch, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Raw, ready-to-eat (RTE) seafood products, such as ceviche, poke, and sushi, have experienced growing demand globally; however, these products have the potential to be contaminated with foodborne pathogens. The objective of this study was to determine the prevalence of Escherichiacoli/coliforms, Salmonella, and Listeria in ceviche, poke, and sushi dishes sold at the retail level in Orange County, CA, USA. Additional organisms detected during testing were also considered in the results. A total of 105 raw, RTE samples of ceviche, poke, and sushi were collected from restaurants and grocery stores in Orange County, CA. Samples were tested for Salmonella …

Antimicrobial Resistance And Epidemiology Of Salmonella Serotype Kentucky Infections—United States, 2009–2022, Caroline Snyder

Public Health Theses

Salmonella enterica serotype Kentucky (Salmonella Kentucky) is the predominant serotype found in retail chicken products (U.S. Food and Drug Administration, 2022). Despite its high likelihood to be found in chicken, Salmonella Kentucky has caused few human illnesses relative to other Salmonella serotypes. Since the 1980s, a multidrug-resistant (MDR) Salmonella Kentucky strain has been disseminating from Egypt to other countries. This MDR strain is characterized by resistance determinants including a mutation that confers resistance to fluoroquinolones like ciprofloxacin; ciprofloxacin-resistant Salmonella are classified as high priority pathogens on the World Health Organization’s (WHO) list of priority resistant bacteria. CDC monitors U.S. …

Inhibiting Survival Of Salmonella During Desiccation Through The Use Of Naturally Occurring Signals, Joseph Headrick

Electronic Theses and Dissertations

A rising problem in agriculture is the increase of antibiotic-resistant Salmonella cases associated with chicken eggs, which transmit infection to humans. To counter this, new approaches to combat Salmonella in chickens and desiccated on eggshells are vital in the prevention of human foodborne illness. Disrupting signaling pathways with naturally occurring compounds provides a potential novel avenue for prevention of Salmonella infections, as this would disrupt sensing of these environments and inhibit subsequent optimal gene expression. Starting with signals identified in previous studies, salicylic acid was found to inhibit Salmonella desiccation survival on both eggshells and plastic. To expand upon this, …

Filth Flies As A Vector For Some Pathogenic Bacteria Transfer, Ahmet Buyukyavuz

All Dissertations

Two separate sets of experiments were conducted to determine the transfer of bacteria by flies. An Escherichia coli ampicillin-resistant strain with a fluorescent gene was used during the experiments. The first set of experiments were divided into two trials to measure the transfer of E. coli by fruit flies to apple slices and bologna during short term exposure. Short time exposure (1, 5 and 15 min) of flies to inoculated apple slices were tested in the first trial to determine the transfer of E. coli to flies. No difference (P>0.05) in the number of bacteria transferred to flies were …

A New Method For Reducing Salmonella In Poultry Meat, Regis Yizerwe

Honors Theses

An important approach to decreasing the transmission of Salmonella into the food chain is to treat raw chicken after processing. This can be, and has been done, by using antibiotics and chemical disinfectants. Using antibiotics is not a viable option due to the issue of antimicrobial resistance. Although some chemical disinfectants have been used effectively, they are restricted due to the amounts that can be used safely, the acceptance of their use and resistance to implementation in various parts of the world. This study has revealed that using a naturally occurring saponin–Quillaja Saponaria– in small concentrations under certain conditions of …

Extraintestinal Salmonellosis Unmasked In A Parotid Mass, Kahmalia-Kalee Sada

North Texas GME Research Forum 2023

Salmonella infection can cause a wide range of presentations, predominantly gastrointestinal but occasionally with cardiovascular or other extraintestinal manifestations. The diagnosis of extraintestinal salmonellosis requires a high degree of clinical suspicion and should be considered in patients with deep-seated abscesses. [1]. Typically, these types of infections, are extremely rare, and when revealed are typically seen in the immunocompromised patient with chronic illness, HIV, or Hepatitis. While the incidence of nontyphoid salmonellosis is estimated at over 2 million cases annually, extraintestinal manifestations account for less than 1% of cases. Studies have revealed that individuals with diabetes have risk for salmonellosis due …

From Big Farm To Big Pharma: A Differential Equations Model Of Antibiotic-Resistant Salmonella In Industrial Poultry Populations, Rilyn Mckallip

Honors Theses

Antibiotics are used in poultry production as prophylaxis, curative treatment, and growth promotion. The first use is as prophylaxis, or prevention of common bacterial diseases. The crowded conditions in concentrated animal feeding operations necessitate management of infectious disease to ensure overall animal health and the profitability of such operations. In these farms, between 20,000 and 125,000 birds are raised in shed-like enclosures [3], with an average of less than one square foot of space per chicken [34]. Antibiotics are currently used in chicken farms to manage and prevent common bacterial diseases such as respiratory and digestive tract infections, as well …

Thermal Inactivation Of Salmonella, Escherichia Coli, And Enterococcus Faecium Nrrl B-2354 In Pasta Matrices, Kristi Shannon Gowans

Theses and Dissertations

Limited data are currently available characterizing the thermal resistance of foodborne pathogens in semolina flour and intermediate pasta matrices representative of commercial conditions during mixing, extrusion, and drying. These data are essential to pasta producers seeking to be compliant with federal regulations since Salmonella spp. and Escherichia coli demonstrate survival in wheat flour and dried pasta products. This study investigated the heat resistance of Salmonella, pathogenic E. coli, and E. faecium NRRL B-2354 in raw semolina flour and partially dried pasta intermediates via thermal death time (TDT) studies. This study also assessed the appropriateness of E. faecium NRRL B-2354 as …

Prevalence, Genetic Characterization, And Antibiogram Of Salmonella Enterica Recovered From Buffalo Meat, Takwa Fathy, Amira Zakaria, Samir Abd-Elghany, Khalid Sallam

Mansoura Veterinary Medical Journal

Objective: This study aimed to determine the prevalence, virulence-associated genes and antimicrobial resistance of Salmonella species recovered from buffalo meat at Mansoura city in Egypt. Salmonella virulence genes were detected using polymerase chain reaction targeting invA, stn, and hilA genes.Design: Observational study.Samples: 120 samples.Procedures: A total of 120 buffalo meat samples were bacteriologically analyzed to isolate and characterize the Salmonella spp. and its virulence genes, in addition to their antimicrobial resistance.Results: Thirty (25%) out of 120-samples from buffalo meat were positive Salmonella spp. Out of the 191 phenotypically identified Salmonella isolates, only 58 strains were molecularly confirmed as Salmonella spp. …

Use Of Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba) To Mitigate Salmonella Enterica, Shiga Toxin-Producing Escherichia Coli (Stec) And Aspergillus Flavus In Pet Food Kibbles, Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich

Department of Food Science and Technology: Faculty Publications

Post-processing operations of extruded pet food kibbles involve coating the product with fats and flavorings. These processes increase the risk for cross-contamination with food-borne pathogens such as Salmonella and Shiga toxin-producing Escherichia coli (STEC), and mycotoxin-producing molds such as Aspergillus spp. after the thermal kill step. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WD-MAX™, against Salmonella enterica, STEC and Aspergillus flavus when used as a coating on pet food kibbles were evaluated. Using canola oil and dry dog digest as fat and flavor …

Identification Of Small-Molecule Inhibitors Of The Salmonella Frab Deglycase Using A Live-Cell Assay, Anice Sabag-Daigle, Erin F Boulanger, Pankajavalli Thirugnanasambantham, Jamison D Law, Alex J Bogard, Edward J Behrman, Venkat Gopalan, Brian M M Ahmer

Student and Faculty Publications

Nontyphoidal salmonellosis is one of the most significant foodborne diseases in the United States and globally. There are no vaccines available for human use to prevent this disease, and only broad-spectrum antibiotics are available to treat complicated cases of the disease. However, antibiotic resistance is on the rise and new therapeutics are needed. We previously identified the Salmonella fraB gene, that mutation of causes attenuation of fitness in the murine gastrointestinal tract. The FraB gene product is encoded in an operon responsible for the uptake and utilization of fructose-asparagine (F-Asn), an Amadori product found in several human foods. Mutations in …

Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich

Department of Food Science and Technology: Faculty Publications

Contaminated surfaces can transmit pathogens to food in industrial and domestic food-handling environments. Exposure to pathogens on food contact surfaces may take place via the cross-contamination of pathogens during postprocessing activities. Formaldehyde-based commercial sanitizers in recent years are less commonly being used within food manufacturing facilities due to consumer perception and labeling concerns. There is interest in investigating clean-label, food-safe components for use on food contact surfaces to mitigate contamination from pathogenic bacteria, including Salmonella. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WD-MAX™, …

Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich

Department of Food Science and Technology: Faculty Publications

Contaminated surfaces can transmit pathogens to food in industrial and domestic food-handling environments. Exposure to pathogens on food contact surfaces may take place via the cross-contamination of pathogens during postprocessing activities. Formaldehyde-based commercial sanitizers in recent years are less commonly being used within food manufacturing facilities due to consumer perception and labeling concerns. There is interest in investigating clean-label, food-safe components for use on food contact surfaces to mitigate contamination from pathogenic bacteria, including Salmonella. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WDMAX …

Whole Genome Analysis And Molecular Traceability Study Of Salmonella In Fresh Livestock And Poultry Meat, Sheng Song, Han Gao, Li Yan, Kun-Peng Guo, Hai-Yun Zhang, Fang-Bin Wang

Food and Machinery

In order to investigate the contamination status of Sal-monella in fresh pork and fresh chicken, and research its whole genome characteristics and molecular traceability, the laboratory randomly collected 24 fresh pork and 27 fresh chicken from the fresh market in Changsha, were inspected according to “National Food Safety Standard Food microbiological examination: Salmonella” GB 4789.4—2016. The results showed that in 24 randomly selected fresh pork, 20 samples were detected, with the detection rate of 83.3%; in 27 randomly selected fresh chicken, 20 samples were detected, and the detected rate is 74.1%; the total detection rate was 78.4%. The results …

Evaluation Of Irish Consumers’ Knowledge Of Salmonellosis And Foodhandling Practices, Ann Conway, Olugbenga Ehuwa, Meabh Manning, Aine Maye, Fintan Moran, Amit K. Jaiswal, Swarna Jaiswal

Articles

Salmonella is one of numerous food-borne pathogens that could possibly pose a major threat to global food safety. Salmonella is primarily associated with foods such as poultry, eggs, vegetables, and some dairy products. However, infected food handlers and faecal contaminated environments are also significant sources and reservoirs of this pathogen. This study comprehensively evaluated the Irish consumers’ food safety knowledge by exploring their knowledge level, practices and attitudes regarding raw meat handling, cross-contamination while handling different types of food products, and knowledge of Salmonella risk and associated food-handling practices. The online SurveyMonkey tool was used to distribute a quantitative survey …